为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Cardiovascular Angiogenesis Adhesion / ECM Integrins

Anti-FAK (phospho Y861)抗体(ab4804)

  • Datasheet
Reviews (2)Q&A (1)References (3)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-FAK (phospho Y861) antibody (ab4804)
  • Western blot - Anti-FAK (phospho Y861) antibody (ab4804)

Key features and details

  • Rabbit polyclonal to FAK (phospho Y861)
  • Suitable for: WB
  • Reacts with: Mouse, Chicken, Human
  • Isotype: IgG

你可能会感兴趣

蛋白
Product image
Recombinant human FAK protein (ab105909)
二抗
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

查看更多相关产品

概述

  • 产品名称

    Anti-FAK (phospho Y861)抗体
    参阅全部 FAK 一抗
  • 描述

    兔多克隆抗体to FAK (phospho Y861)
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Mouse, Chicken, Human
    预测可用于: Rat, Xenopus laevis
  • 免疫原

    Synthetic peptide (Human) derived from the region of human Focal Adhesion Kinase that contains tyrosine 861. The sequence is conserved in human, mouse, rat, chicken and frog.

  • 常规说明


    Focal Adhesion Kinase is a 125 kDa non-receptor protein tyrosine kinase that is a substrate for Src and a key element in growth factor and integrin signalling. Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Tyr861 of Focal Adhesion Kinase is a major Src phosphorylation site that allows Focal Adhesion Kinase to bind to integrins and is also involved in cancer.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Focal Adhesion Kinase protein, and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine (irrespective of the sequence). The final product is generated by affinity chromatography using a Focal Adhesion Kinase-derived peptide that is phosphorylated at tyrosine 861.
  • Primary antibody说明

    Focal Adhesion Kinase is a 125 kDa non-receptor protein tyrosine kinase that is a substrate for Src and a key element in growth factor and integrin signalling. Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Tyr861 of Focal Adhesion Kinase is a major Src phosphorylation site that allows Focal Adhesion Kinase to bind to integrins and is also involved in cancer.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Integrins
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • FAK / PYK
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Focal Adhesions
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • FAK family

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human FAK protein (ab105909)

应用

Our Abpromise guarantee covers the use of ab4804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 119 kDa.

靶标

  • 功能

    Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
  • 组织特异性

    Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • 结构域

    The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
    The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
  • 翻译后修饰

    Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
  • 细胞定位

    Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
  • Target information above from: UniProt accession Q05397 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 396416 Chicken
    • Entrez Gene: 5747 Human
    • Entrez Gene: 14083 Mouse
    • Entrez Gene: 25614 Rat
    • Entrez Gene: 399286 Xenopus laevis
    • Omim: 600758 Human
    • SwissProt: Q00944 Chicken
    • SwissProt: Q05397 Human
    • SwissProt: P34152 Mouse
    • SwissProt: O35346 Rat
    • SwissProt: Q91738 Xenopus laevis
    • Unigene: 395482 Human
    • Unigene: 254494 Mouse
    • Unigene: 2809 Rat
    • Unigene: 6819 Xenopus laevis
    see all
  • 别名

    • FADK 1 antibody
    • FADK antibody
    • FAK related non kinase polypeptide antibody
    • FAK1 antibody
    • FAK1_HUMAN antibody
    • Focal adhesion kinase 1 antibody
    • Focal adhesion Kinase antibody
    • Focal adhesion kinase isoform FAK Del33 antibody
    • Focal adhesion kinase related nonkinase antibody
    • FRNK antibody
    • p125FAK antibody
    • pp125FAK antibody
    • PPP1R71 antibody
    • Protein phosphatase 1 regulatory subunit 71 antibody
    • Protein tyrosine kinase 2 antibody
    • Protein-tyrosine kinase 2 antibody
    • Ptk2 antibody
    • PTK2 protein tyrosine kinase 2 antibody
    see all

图片

  • Western blot - Anti-FAK (phospho Y861) antibody (ab4804)
    Western blot - Anti-FAK (phospho Y861) antibody (ab4804)
    Peptide Competition: Cell extracts prepared from chick embryo fibroblasts expressing FAK and plated on fibronectin were resolved by SDS-PAGE on a 10% Tris-glycine gel. The proteins then were transferred to nitrocellulose and incubated with 0.50 µg/mL ab4804 antibody, following prior incubation with: (1) the phosphopeptide immunogen, (2) a generic phosphotyrosine containing peptide, (3) the non-phosphorylated peptide corresponding to the phosphopeptide, and (4) no peptide. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, demonstrating the specificity of the ab4804 antibody for this phosphorylated residue. Peptide Competition: Cell extracts prepared from chick embryo fibroblasts expressing FAK and plated on fibronectin were resolved by SDS-PAGE on a 10% T
  • Western blot - Anti-FAK (phospho Y861) antibody (ab4804)
    Western blot - Anti-FAK (phospho Y861) antibody (ab4804)Image courtesy of an anonymous Abreview.
    All lanes : Anti-FAK (phospho Y861) antibody (ab4804) at 1/1000 dilution

    Lane 1 : Whole cell lysate prepared from human MDA-MB-231 breast cancer cells, un-treated
    Lane 2 : Whole cell lysate prepared from human MDA-MB-231 breast cancer cells, treated for 1hr with 2.5uM AZD0530 src inhibitor
    Lane 3 : Whole cell lysate prepared from human MDA-MB-231 breast cancer cells, treated for 1hr with 5uM AZD0530 src inhibitor

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 119 kDa
    Observed band size: 125 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes


    Primary antibody incubated for 16 hours at 4°C.
    Blocking step was performed using 5% milk for 1 hour at 25°C.

    See Abreview

实验方案

  • Peptide Competition Experiment

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (3)

    发表研究结果有使用 ab4804?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4804 被引用在 3 文献中.

    • Gill MB  et al. KSHV-TK is a tyrosine kinase that disrupts focal adhesions and induces Rho-mediated cell contraction. EMBO J 34:448-65 (2015). PubMed: 25471072
    • Ding LW  et al. LNK (SH2B3): paradoxical effects in ovarian cancer. Oncogene N/A:N/A (2014). PubMed: 24704825
    • Tan CL  et al. Integrin activation promotes axon growth on inhibitory chondroitin sulfate proteoglycans by enhancing integrin signaling. J Neurosci 31:6289-95 (2011). ICC/IF ; Human . PubMed: 21525268

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-FAK (phospho Y861) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human Synoviocyte fibroblast-like cell)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    20 µg
    Treatment
    High glucose 500mg/dl D-glucose for 24hrs
    Specification
    Human Synoviocyte fibroblast-like cell
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 27 2016

    Question

    Wir hatten von Abcam vor einiger Zeit einen Antikörper bestellt, um diesen
    in Maus zu testen da er für humanes ApoER2 ausgeschrieben ist.
    Dies hatten wir nun auch getan, allerdings ohne Erfolg (siehe Anhang).
    Nun wollten wir dieses Ergebnis auf der Abcam Homepage hoch laden,
    allerdings bin ich mir nicht sicher ob ich bei der richtigen Seite gelandet
    bin. Ich konnte mir einen Account anlegen, allerdings habe ich nichts
    gefunden wo ich den DISCOUNT CODE eingeben konnte, und ich weiß nicht wo es
    genau hervor geht, dass dieser Antikörper zum Testen bestellt wurde.
    Können Sie mir hierbei weiterhelfen?
    Wir haben das Gefühl dass der Antikörper generell nicht funktioniert, da die
    humane und murine ApoER2 Ektodomäne eine Sequenzhomologie von über 80%
    aufweisen. Aus diesem Grund ist es komisch, dass so gar kein Signal zu
    detektieren war. Allerdings haben wir für den Abcam ApoER2 Antikörper keine
    positiv Kontrolle, da wir kein humanes ApoER2 Konstrukt haben.
    Leider ist es uns in letzter Zeit mit einem weiteren Abcam Antikörper so
    ergangen, dass er nicht funktionierte (pFAK; ab4804). Dieser weist zwar eine
    Bande auf aber nicht bei den vorhergesagten 125 kDa, sondern bei einer Höhe
    von ca. 95 kDa.

    Mit freundlichen Grüßen

    Read More

    Abcam community

    Verified customer

    Asked on Oct 19 2012

    Answer

    Vielen Dank für Ihre Anfrage und Ihr Feedback.

    Um den Gutschein Code einlösen zu können, müssen Sie eine Abreview verfassen. Auf dem Datenblatt von ab58216 gibt es mehrere Links über die das gemacht werden kann:

    Direkt unter dem Antikörpernamen oder wenn Sie auf den reiter (tab) "Abreviews" klicken und dann "submit an Abreview".

    Bitte geben Sie den Gutschein Code in den "additional notes" in der Abreviewform an damit er aktiviert wird, wenn der Abreview eingereicht wird.

    Danach kann der Code auf einer neuen Bestellung benutzt. werden.

    Es tut mir leid zu hören, dass Sie Probleme mit ab4804 in WB haben.

    Ich habe unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Fragebogens erhalten wir alle nötigen Informationen über Ihre Proben und Ihr Protokoll. Sobald Sie dieses Formular an uns zurückgeschickt haben, werden wir uns Ihr Protokoll ansehen und möglichst Veränderungsvorschläge machen, die Ihre Ergebnisse verbessern werden.

    Für diesen Antikörper gibt es ein speziell empfohlenes Protokoll:

    https://www.abcam.com/assets/popups/popup_datasheet_protocols_printer_friendly.cfm?pid=310&intAbId=4804

    Falls sich herausstellt, dass der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben und er innerhalb der letzten sechs Monaten gekauft wurde, werden wir Ihnen gerne einen Ersatz oder eine Gutschrift schicken.
    Ich freue mich, bald wieder von Ihnen zu hören.

    Read More

    Abcam Scientific Support

    回复于 Oct 19 2012

    Western blot abreview for Anti-FAK (phospho Y861) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MDA-MB-231 human breast cancer cells)
    Loading amount
    25 µg
    Specification
    MDA-MB-231 human breast cancer cells
    Treatment
    either untreated or treated with 2.5 or 5uM src inhibitor AZD0530
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 17 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    ?ICP?14046373?-1

    ????? 31011502007413?

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有