重组Anti-FAK (phospho Y576)抗体[EP1897Y] (ab76120)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1897Y] to FAK (phospho Y576)
- Suitable for: Flow Cyt (Intra), Dot blot, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-FAK (phospho Y576)抗体[EP1897Y]
参阅全部 FAK 一抗 -
描述
兔单克隆抗体[EP1897Y] to FAK (phospho Y576) -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), Dot blot, WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa cell lysate and 3T3 cell lysates treated with pervanadate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1897Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76120于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/50 - 1/400.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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Dot blot |
1/1000.
|
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WB |
1/1000 - 1/100000. Detects a band of approximately 125 kDa (predicted molecular weight: 119 kDa).
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说明 |
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Flow Cyt (Intra)
1/50 - 1/400. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Dot blot
1/1000. |
WB
1/1000 - 1/100000. Detects a band of approximately 125 kDa (predicted molecular weight: 119 kDa). |
靶标
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功能
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity. -
组织特异性
Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain. -
结构域
The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions. -
翻译后修饰
Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1. -
细胞定位
Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions. - Information by UniProt
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数据库链接
- Entrez Gene: 5747 Human
- Entrez Gene: 14083 Mouse
- Entrez Gene: 25614 Rat
- Omim: 600758 Human
- SwissProt: Q05397 Human
- SwissProt: P34152 Mouse
- SwissProt: O35346 Rat
- Unigene: 395482 Human
see all -
别名
- FADK 1 antibody
- FADK antibody
- FAK related non kinase polypeptide antibody
see all
图片
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All lanes : Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120) at 1/1000 dilution (Purified)
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa treated with 50nM pervanadate for 60 minutes whole cell lysate
Lane 3 : HeLa treated with 50nM pervanadate for 60 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 119 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?Blocking/Diluting Buffer and concentration 5% NFDM/TBST
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Intracellular Flow Cytometry analysis of HeLa cells treated with 1mM Pervanadate for 30mins cells labeling FAK (phospho Y576)with ab76120 at 1/400 dilution (0.1µg) (red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730 / Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Dot blot analysis of FAK (phospho Y576) phospho peptide (Lane 1) and FAK non-phospho peptide (Lane 2) labelling FAK (phospho Y576) phospho peptide with ab76120 at a dilution of 1/1000 dilution (0.441ug/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution.
Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST .
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All lanes : Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2 : NIH/3T3 treated with 10mM pervanadate for 20 minutes whole cell lysate
Lane 3 : NIH/3T3 treated with 10mM pervanadate for 20 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 119 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?Blocking/Diluting Buffer and concentration 5% NFDM/TBST
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All lanes : Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120) at 1/50000 dilution
Lane 1 : 3T3 cell lysates untreated
Lane 2 : 3T3 cell lysates treated with pervanadate.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 119 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab76120 被引用在 3 文献中.
- Wu M et al. FSTL1 promotes growth and metastasis in gastric cancer by activating AKT related pathway and predicts poor survival. Am J Cancer Res 11:712-728 (2021). PubMed: 33791149
- Luo X et al. Bioinformatics analysis of microarray profiling identifies the mechanism of focal adhesion kinase signalling pathway in proliferation and apoptosis of breast cancer cells modulated by green tea polyphenol epigallocatechin 3-gallate. J Pharm Pharmacol 70:1606-1618 (2018). PubMed: 30187481
- Strell C et al. Neutrophil granulocytes promote the migratory activity of MDA-MB-468 human breast carcinoma cells via ICAM-1. Exp Cell Res 316:138-48 (2010). PubMed: 19747913