Anti-FAK抗体(ab131435)
Key features and details
- Rabbit polyclonal to FAK
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-FAK抗体
参阅全部 FAK 一抗 -
描述
兔多克隆抗体to FAK -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide conjugated to KLH, derived from around amino acids 923-927 (
K-V-Y-E-N
) of Human FAK (UniProt Q05397). -
阳性对照
- HeLa cell extract; HeLa cells; Human breast carcinoma tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131435于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 125 kDa (predicted molecular weight: 119 kDa).
|
|
IHC-P | (1) |
1/50 - 1/100.
|
ICC/IF |
1/100 - 1/200.
|
说明 |
---|
WB
1/500 - 1/1000. Detects a band of approximately 125 kDa (predicted molecular weight: 119 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity. -
组织特异性
Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain. -
结构域
The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions. -
翻译后修饰
Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1. -
细胞定位
Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions. - Information by UniProt
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数据库链接
- Entrez Gene: 5747 Human
- Omim: 600758 Human
- SwissProt: Q05397 Human
- Unigene: 395482 Human
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别名
- FADK 1 antibody
- FADK antibody
- FAK related non kinase polypeptide antibody
see all
图片
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All lanes : Anti-FAK antibody (ab131435) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : HeLa cell extract with blocking peptide
Predicted band size: 119 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling FAK with ab131435 at 1/50 dilution. Right panel was preincubated with blocking peptide.
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Immunofluorescent analysis of methanol-fixed HeLa cells staining FAK with ab131435 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab131435 被引用在 16 文献中.
- Figueira I et al. Picturing Breast Cancer Brain Metastasis Development to Unravel Molecular Players and Cellular Crosstalk. Cancers (Basel) 13:N/A (2021). PubMed: 33671551
- Xu K et al. Tenascin-C regulates migration of SOX10 tendon stem cells via integrin-α9 for promoting patellar tendon remodeling. Biofactors 47:768-777 (2021). PubMed: 34058037
- Godinho-Pereira J et al. Behind Brain Metastases Formation: Cellular and Molecular Alterations and Blood-Brain Barrier Disruption. Int J Mol Sci 22:N/A (2021). PubMed: 34209088
- Abula Y et al. Desmoglein 3 contributes to tumorigenicity of pancreatic ductal adenocarcinoma through activating Src-FAK signaling. Anim Cells Syst (Seoul) 25:195-202 (2021). PubMed: 34262662
- Du Q et al. High Expression of Integrin a3 Predicts Poor Prognosis and Promotes Tumor Metastasis and Angiogenesis by Activating the c-Src/Extracellular Signal-Regulated Protein Kinase/Focal Adhesion Kinase Signaling Pathway in Cervical Cancer. Front Oncol 10:36 (2020). PubMed: 32117712