Key features and details
- Mouse monoclonal [63D5] to FAK
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
参阅全部 FAK 一抗
描述小鼠单克隆抗体[63D5] to FAK
经测试应用适用于: WB, Flow Cytmore details
种属反应性与反应: Mouse, Rat, Human
Recombinant fragment corresponding to amino acids 706-1052 of Human FAK with a His tag
- NIH 3T3 cytoplasmic extract lysate (ab14873) can be used as a positive control in WB. A431, HeLa, NIH2T3 and C6 cell lysates
This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR188357 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Preservative: 0.03% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, HEPES
Concentration information loading...
纯度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab72140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 119 kDa (predicted molecular weight: 119 kDa).|
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
功能Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
组织特异性Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
结构域The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
翻译后修饰Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
细胞定位Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
- Information by UniProt
- FADK 1 antibody
- FADK antibody
- FAK related non kinase polypeptide antibody
Overlay histogram showing HeLa cells stained with ab72140 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab72140, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
All lanes : Anti-FAK antibody [63D5] (ab72140) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : NIH3T3 cell lysate
Lane 4 : C6 cell lysate
Predicted band size: 119 kDa
Observed band size: 119 kDa
ab72140 被引用在 5 文献中.
- Pucciarelli D et al. Hypoxia increases the heterogeneity of melanoma cell populations and affects the response to vemurafenib. Mol Med Rep 13:3281-8 (2016). PubMed: 26936534
- Lin Y & Sun Z Antiaging Gene Klotho Attenuates Pancreatic ß-Cell Apoptosis in Type 1 Diabetes. Diabetes 64:4298-311 (2015). WB . PubMed: 26340932
- Cleghorn WM et al. Arrestins regulate cell spreading and motility via focal adhesion dynamics. Mol Biol Cell 26:622-35 (2015). PubMed: 25540425
- Yu S et al. Systems pharmacology of mifepristone (RU486) reveals its 47 hub targets and network: comprehensive analysis and pharmacological focus on FAK-Src-Paxillin complex. Sci Rep 5:7830 (2015). PubMed: 25597938
- Prévilon M et al. Comparative differential proteomic profiles of nonfailing and failing hearts after in vivo thoracic aortic constriction in mice overexpressing FKBP12.6. Physiol Rep 1:e00039 (2013). WB . PubMed: 24303125