产品名称Anti-FADD (phospho S194)抗体[EPR1816Y] - BSA and Azide free
参阅全部 FADD 一抗
描述兔单克隆抗体[EPR1816Y] to FADD (phospho S194) - BSA and Azide free
经测试应用适用于: WB, IPmore details
Synthetic peptide within Human FADD aa 150-250. The exact sequence is proprietary.
- WB: Jurkat cells treated with calyculin A.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab232047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 23 kDa).|
|IP||Use at an assay dependent concentration.|
功能Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
组织特异性Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
序列相似性Contains 1 death domain.
Contains 1 DED (death effector) domain.
结构域Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
- Information by UniProt
- FADD antibody
- FADD protein antibody
- FADD_HUMAN antibody
All lanes : Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475) at 1/500 dilution
Lane 1 : Un-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : Calyculin A treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 23 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68475).
ab232047 has not yet been referenced specifically in any publications.