The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 23 kDa).
Use at an assay dependent concentration.
Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
Contains 1 death domain. Contains 1 DED (death effector) domain.
Contains a death domain involved in the binding of the corresponding domain within Fas receptor.