重组Anti-FADD (phospho S194)抗体[EPR1816Y] - BSA and Azide free (ab232047)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1816Y] to FADD (phospho S194) - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-FADD (phospho S194)抗体[EPR1816Y] - BSA and Azide free
参阅全部 FADD 一抗 -
描述
兔单克隆抗体[EPR1816Y] to FADD (phospho S194) - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Jurkat cells treated with calyculin A.
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常规说明
ab232047 is the carrier-free version of ab68475.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1816Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab232047于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 23 kDa).
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 23 kDa). |
靶标
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功能
Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. -
组织特异性
Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes. -
序列相似性
Contains 1 death domain.
Contains 1 DED (death effector) domain. -
结构域
Contains a death domain involved in the binding of the corresponding domain within Fas receptor. - Information by UniProt
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数据库链接
- Entrez Gene: 8772 Human
- Omim: 602457 Human
- SwissProt: Q13158 Human
- Unigene: 86131 Human
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别名
- FADD antibody
- FADD protein antibody
- FADD_HUMAN antibody
see all
图片
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All lanes : Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475) at 1/500 dilution
Lane 1 : Un-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : Calyculin A treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 23 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68475).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab232047 尚未被引用在任何文献中。