Anti-F4/80抗体[F4/80] - Macrophage Marker (ab90247)
Key features and details
- Rat monoclonal [F4/80] to F4/80 - Macrophage Marker
- Suitable for: ICC/IF
- Reacts with: Mouse
- Isotype: IgG2b
Related conjugates and formulations
概述
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产品名称
Anti-F4/80抗体[F4/80] - Macrophage Marker
参阅全部 F4/80 一抗 -
描述
大鼠单克隆抗体[F4/80] to F4/80 - Macrophage Marker -
宿主
Rat -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: Raw 264.7 cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
F4/80 -
骨髓瘤
NS1 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab90247于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 5 - 10 µg/ml.
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说明 |
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ICC/IF
Use a concentration of 5 - 10 µg/ml. |
靶标
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功能
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
组织特异性
Expression is restricted to eosinophils. -
序列相似性
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 13733 Mouse
- SwissProt: Q61549 Mouse
- Unigene: 2254 Mouse
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别名
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
图片
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ab90247 staining F4/80 in Raw264.7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab90247 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150165, Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
实验方案
数据表及文件
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Datasheet download
文献 (30)
ab90247 被引用在 30 文献中.
- Fischer A et al. Neutrophils direct preexisting matrix to initiate repair in damaged tissues. Nat Immunol 23:518-531 (2022). PubMed: 35354953
- Xu Q et al. The flavonoid procyanidin C1 has senotherapeutic activity and increases lifespan in mice. Nat Metab 3:1706-1726 (2021). PubMed: 34873338
- Zhang S et al. Construction and Optimization of an Endometrial Injury Model in Mice by Transcervical Ethanol Perfusion. Reprod Sci 28:693-702 (2021). PubMed: 32939736
- Jiang D et al. Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin. Nat Commun 11:5653 (2020). PubMed: 33159076
- Rahman FA et al. Impaired ECM Remodeling and Macrophage Activity Define Necrosis and Regeneration Following Damage in Aged Skeletal Muscle. Int J Mol Sci 21:N/A (2020). PubMed: 32605082