Anti-F4/80抗体(ab100790)
Key features and details
- Rabbit polyclonal to F4/80
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-F4/80抗体
参阅全部 F4/80 一抗 -
描述
兔多克隆抗体to F4/80 -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
Synthetic peptide within Mouse F4/80 aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: Q61549 -
阳性对照
- IHC-P: Human Lung tissue, Mouse Liver tissue
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab100790于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (4) |
1/100.
Boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min. This product may benefit from the use of freshly cut tissue. |
| 说明 |
|---|
|
IHC-P
1/100. Boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min. This product may benefit from the use of freshly cut tissue. |
靶标
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功能
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
组织特异性
Expression is restricted to eosinophils. -
序列相似性
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2015 Human
- Entrez Gene: 13733 Mouse
- Entrez Gene: 316137 Rat
- Omim: 600493 Human
- SwissProt: Q14246 Human
- SwissProt: Q61549 Mouse
- SwissProt: Q5Y4N8 Rat
- Unigene: 2375 Human
see all -
别名
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody (ab100790)
Staining of F4/80 in a Formalin/PFA-fixed paraffin-embedded section of human lung using ab100790 at a dilution of 1/100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody (ab100790)Staining of F4/80 in a Formalin/PFA-fixed paraffin-embedded section of human lung using ab100790 at a dilution of 1/100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody (ab100790)IHC image of F4/80 staining in a section of formalin-fixed paraffin-embedded normal mouse liver performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab100790, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (67)
ab100790 被引用在 67 文献中.
- Maruoka H et al. Effects of neuromuscular electrical stimulation on pulmonary alveola and cytokines in chronic obstructive pulmonary disease (COPD) and skeletal muscle atrophy model mice. J Phys Ther Sci 33:1-8 (2021). PubMed: 33519066
- Shimizu Y et al. Oxidative stress and Liver X Receptor agonist induce hepatocellular carcinoma in Non-alcoholic steatohepatitis model. J Gastroenterol Hepatol 36:800-810 (2021). PubMed: 32870526
- Lei J et al. M2 macrophages-derived exosomal microRNA-501-3p promotes the progression of lung cancer via targeting WD repeat domain 82. Cancer Cell Int 21:91 (2021). PubMed: 33546686
- Gong W et al. CCL4-mediated targeting of spleen tyrosine kinase (Syk) inhibitor using nanoparticles alleviates inflammatory bowel disease. Clin Transl Med 11:e339 (2021). PubMed: 33634985
- He L et al. Lactucopicrin Inhibits Cytoplasmic Dynein-Mediated NF-?B Activation in Inflammated Macrophages and Alleviates Atherogenesis in Apolipoprotein E-Deficient Mice. Mol Nutr Food Res 65:e2000989 (2021). PubMed: 33377310