- 数据表
- 具体的参考文献 (31)
- 实验方案
概述
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产品名称Anti-F-actin抗体[NH3]
参阅全部 F-actin 一抗 -
描述小鼠单克隆抗体[NH3] to F-actin
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宿主Mouse
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特异性Clone NH3 recognises human Filamentous actin (F-actin) and reportedly also recognizes Globular actin (G-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.
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经测试应用适用于: Flow Cyt, ICC/IF, IHC-R, WB, ELISA, IHC-Fr, IPmore details
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种属反应性与反应: Mouse, Rat, Rabbit, Human
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免疫原
Human monocytes and U397 cell line.
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表位This antibody is reported to recognise actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule.
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阳性对照
- gastrointestinal tissue
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.1% Sodium azide
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Concentration information loading... -
纯度Purified IgM
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纯化说明Purified IgM prepared from tissue culture supernatant.
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克隆单克隆
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克隆编号NH3
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骨髓瘤NS1
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同种型IgM
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轻链类型unknown
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研究领域
相关产品
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Compatible Secondaries
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Immunohistochemistry kits
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Related Products
应用
Our Abpromise guarantee covers the use of ab205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | 1/10. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | 1/100. | |
| IHC-R | Use at an assay dependent concentration. | |
| WB | 1/100 - 1/500. Detects a band of approximately 43 kDa. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary. | |
| ELISA | 1/10. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. PubMed: 27336173 |
靶标
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功能Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
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疾病相关Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
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序列相似性Belongs to the actin family.
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翻译后修饰ISGylated.
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细胞定位Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
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数据库链接
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- SwissProt: P29751 Rabbit
see all -
别名
- ACTB antibody
- ACTB_HUMAN antibody
- Actin, cytoplasmic 1, N-terminally processed antibody
see all
图片
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![Western blot - Anti-F-actin antibody [NH3] (ab205)](https://www.abcam.cn/ps/products/0/ab205/Images/ab205_2.jpg)
Western blot - Anti-F-actin antibody [NH3] (ab205)This image is courtesy of an anonymous AbreviewAnti-F-actin antibody [NH3] (ab205) at 1/100 dilution + Human pancreatic cancer cell line, whole cell lysate at 20 µg
Secondary
HRP conjugated goat anti-mouse antibody at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 43 kDa (why is the actual band size different from the predicted?)
Exposure time: 1 minute
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![Immunocytochemistry/ Immunofluorescence - Anti-F-actin antibody [NH3] (ab205)](https://www.abcam.cn/ps/products/0/ab205/Images/ab205-274549-ab20526885895.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-F-actin antibody [NH3] (ab205)Image from Ibrahim, Ahmed S. et al. Oncotarget 7.8 (2016): 8532–8545. doi: 10.18632/oncotarget.7384, Fig 6D.Immunocytochemistry/ Immunofluorescence analysis of ARPE cells labelling F-actin with ab205. Cells were fixed in 4% paraformaldehyde for 10 min, washed with PBS, and blocked for one hour. Cells were then incubated for 3h at 37°C with Anti-F-actin antibody [NH3] (ab205) at 1/200 dilution. Thereafter, cells were washed 3 times with PBS containing 0.3% Triton-X, incubated with secondary antibody and coverslipped with Fluoroshield containing DAPI as a counter stain. Immunofluorescent signals were detected by confocal microscopy.
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![Immunohistochemistry (Resin sections) - Anti-F-actin antibody [NH3] (ab205)](https://www.abcam.cn/ps/products/0/ab205/Images/ab205-274547-ab20537093.jpg)
Immunohistochemistry (Resin sections) - Anti-F-actin antibody [NH3] (ab205)This image is courtesy of an anonymous abreview.Immunohistochemistry (Resin sections) analysis of mouse bone tissue cells labeling F-actin with ab205 at 1/200 dilution. Mouse tibia bone samples were fixed in neutral buffered formalin for 72 hours at room temperature, embedded undecalcified in plastic and sectioned at 5 microns. A polyclonal goat anti-mouse Alexa Fluor® 488 secondary antibody was used at 1/700 dilution. The image shows a 1 micron thick z-projection of the chondrocytes in the proximal tibia growth plate.
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![Western blot - Anti-F-actin antibody [NH3] (ab205)](https://www.abcam.cn/ps/products/0/ab205/Images/ab205-274545-ab20544330.jpg)
Western blot - Anti-F-actin antibody [NH3] (ab205)This image is courtesy of an anonymous abreview.Anti-F-actin antibody [NH3] (ab205) at 1/500 dilution + CNS whole tissue lysate at 30 µg
Secondary
Goat anti-mouse HRP conjugate at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 43 kDa (why is the actual band size different from the predicted?)
Exposure time: 5 minutesBlocking: 5% milk for 1 hour at 25°C.
Incubation with primary antibody for 15 hours at 4°C in 5%milk+ PBS+0.1% Tween 20.
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![Immunocytochemistry/ Immunofluorescence - Anti-F-actin antibody [NH3] (ab205)](https://www.abcam.cn/ps/products/0/ab205/Images/ab205-274562-ab20555840.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-F-actin antibody [NH3] (ab205)This image is courtesy of an anonymous abreview.Immunocytochemistry/ Immunofluorescence analysis of mouse cortical neurons labeling F-actin with ab205 at 1/100 dilution. The cells were fixed with Ethanol and permeabilized with 0.2% Triton X-100. A polyclonal goat anti-mouse Cy3 conjugated secondary antibody was used at 1/200 dilution.
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![Immunohistochemistry (Frozen sections) - Anti-F-actin antibody [NH3] (ab205)](https://www.abcam.cn/ps/products/0/ab205/Images/F-actin-Primary-antibodies-ab205-1.jpg)
Immunohistochemistry (Frozen sections) - Anti-F-actin antibody [NH3] (ab205)Image courtesy of an Abreview submitted by Jamie Richards.ab205 staining F-actin in rat brain tissue by Immunohistochemistry (frozen sections). Tissue was fixed with paraformaldehyde and then blocked with 2% BSA for 1 hour followed by incubation with the primary antibody at a 1/25 dilution for 24 hours. A biotin-conjugated goat anti-mouse IgM was used as secondary antibody at a 1/400 dilution. No permeabilisation step was used.
实验方案
文献
This product has been referenced in:
- Shi C et al. Yap promotes hepatocellular carcinoma metastasis and mobilization via governing cofilin/F-actin/lamellipodium axis by regulation of JNK/Bnip3/SERCA/CaMKII pathways. Redox Biol 14:59-71 (2018). Read more (PubMed: 28869833) »
- Yan H et al. Yap regulates gastric cancer survival and migration via SIRT1/Mfn2/mitophagy. Oncol Rep 39:1671-1681 (2018). Read more (PubMed: 29436693) »
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