Anti-F-actin抗体[NH3] (ab205)

Reviews (10)Q&A (26)References (96)

Key features and details

  • Mouse monoclonal [NH3] to F-actin
  • Reacts with: Rat, Rabbit, Human
  • Isotype: IgM

概述

  • 产品名称

    Anti-F-actin抗体[NH3]
    参阅全部 F-actin 一抗

  • 描述

    小鼠单克隆抗体[NH3] to F-actin

  • 宿主

    Mouse

  • 特异性

    Clone NH3 recognises human Filamentous actin (F-actin) and reportedly also recognizes Globular actin (G-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.

  • 种属反应性

    与反应: Rat, Rabbit, Human

  • 免疫原

    Tissue, cells or virus corresponding to F-actin. Human monocytes and U397 cell line.

  • 表位

    This antibody is reported to recognise actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule.

  • 阳性对照

    • gastrointestinal tissue

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

靶标

  • 功能

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

  • 疾病相关

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.

  • 序列相似性

    Belongs to the actin family.

  • 翻译后修饰

    ISGylated.

  • 细胞定位

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Beta-actin antibody
    • f actin antibody
    • Filamentous actin antibody
    see all

文献 (96)

发表研究结果有使用 ab205？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab205 被引用在 96 文献中.

  • Liu WL  et al. High Mobility Group Box 1 Promotes Lung Cancer Cell Migration and Motility via Regulation of Dynamin-Related Protein 1. Int J Mol Sci 22:N/A (2021). PubMed: 33807275
  • Zhong Y  et al. Inhibiting Th1/2 cells influences hepatic capillarization by adjusting sinusoidal endothelial fenestrae through Rho-ROCK-myosin pathway. Aging (Albany NY) 13:5069-5086 (2021). PubMed: 33535174
  • Kong M  et al. Myocardin-related transcription factor A nuclear translocation contributes to mechanical overload-induced nucleus pulposus fibrosis in rats with intervertebral disc degeneration. Int J Mol Med 48:N/A (2021). PubMed: 33982787
  • Hattori K  et al. The Infantile Leukoencephalopathy-Associated Mutation of C11ORF73/HIKESHI Proteins Generates de novo Interactive Activity with Filamin A, Inhibiting Oligodendroglial Cell Morphological Differentiation. Medicines (Basel) 8:N/A (2021). PubMed: 33535532
  • Zhao H  et al. Effects of Simulated Microgravity on Ultrastructure and Apoptosis of Choroidal Vascular Endothelial Cells. Front Physiol 11:577325 (2020). PubMed: 33536932
View all Publications for this product

客户评价及客户问答

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1-10 of 36 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-F-actin antibody [NH3]

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - NP40
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

提交于 May 29 2019

Western blot abreview for Anti-F-actin antibody [NH3]

 Poor
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
U2OS
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

提交于 Apr 05 2019

Immunohistochemistry (Frozen sections) abreview for Anti-F-actin antibody [NH3]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue (Pancreas PanIN)
Permeabilization
No
Specification
Pancreas PanIN
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Methanol
Read More

Abcam user community

Verified customer

提交于 Jun 27 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-F-actin antibody [NH3]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Derived Breast cancer Cell Line)
Permeabilization
Yes - 0.5 % Triton
Specification
Mouse Derived Breast cancer Cell Line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 26°C
Fixative
Methanol
Read More

Abcam user community

Verified customer

提交于 Aug 17 2017

Western blot abreview for Anti-F-actin antibody [NH3]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Derived Breast Cancer Primary Cell Line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
40 µg
Specification
Mouse Derived Breast Cancer Primary Cell Line
Blocking step
Licor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Read More

Abcam user community

Verified customer

提交于 Aug 14 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-F-actin antibody [NH3]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Cortical Neurons)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Cortical Neurons
Blocking step
casein solution + Tween 20 as blocking agent for 20 minute(s) · Concentration: 0.2% · Temperature: 25°C
Fixative
Ethanol
Read More

Abcam user community

Verified customer

提交于 Aug 04 2016

Western blot abreview for Anti-F-actin antibody [NH3]

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Tissue lysate - whole (CNS)
Specification
CNS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Aug 01 2014

Immunohistochemistry (Resin sections) abreview for Anti-F-actin antibody [NH3]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Resin sections)
Sample
Mouse Tissue sections (Bone Tissue Cells)
Specification
Bone Tissue Cells
Read More

Abcam user community

Verified customer

提交于 Jul 10 2013

Question

Je suis à la recherche d’un anticorps spécifique de l’actine F et un autre spécifique de l’actine monomérique ou actin-G.

J’aimerais un anticorps déjà testé en Immunofluorescence et pas de toxine type phalloidin.

Read More
Answer

Je peux vous proposer ab205, Anti-F-actin antibody [NH3] qui est un monoclonal de souris garanti de fonctionner en immunofluorescence. Cependant, nous n’avons pas testé le taux de toxine dans ce produit et donc nous ne pouvons pas garantir qu’il n’y a pas de toxine type phalloidin présent.


Malheureusement, nous n’avons pas d’anticorps anti-G-actine. Tous nos autres anticorps reconnaissent les deux formes F et G.

Read More

Question

I got the reply from the customer in regards to ab205:
"I have tried, according to your suggestion, to do the IHC experiment again with a different secondary antibody "Alexa Fluor® 488 Goat Anti-Mouse IgG, IgM (H+L)" that recognize both IgG and IgM. This didn't recognize the F-actin antibody and resulted in no positive-specific staining.
At this point I think I have tried almost every step in the attempts to get a positive signal."

Looking forward to your reply.
Thanks in advance for your assistance.

Best Regards,

Read More
Answer

Thank you for your message and for providing this further information.
I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrange a free of charge replacement or refund in compensation.
If you decide to get a free of charge replacement we would recommend it would be beneficial to include a positive control, such as gastrointestinal tissue or brain tissue in the following experiments.
I look forward hearing from you with details of how you would like to proceed.

Read More

1-10 of 36 Abreviews or Q&A

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