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Tags & Cell Markers Subcellular Markers Cytoskeleton Actin

Anti-F-actin抗体[NH3] (ab205)

  • Datasheet
  • SDS
Reviews (10)Q&A (26)References (79)

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Key features and details

  • Mouse monoclonal [NH3] to F-actin
  • Reacts with: Rat, Rabbit, Human
  • Isotype: IgM

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概述

  • 产品名称

    Anti-F-actin抗体[NH3]
    参阅全部 F-actin 一抗
  • 描述

    小鼠单克隆抗体[NH3] to F-actin
  • 宿主

    Mouse
  • 特异性

    Clone NH3 recognises human Filamentous actin (F-actin) and reportedly also recognizes Globular actin (G-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.
  • 种属反应性

    与反应: Rat, Rabbit, Human
  • 免疫原

    Human monocytes and U397 cell line.

  • 表位

    This antibody is reported to recognise actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule.
  • 阳性对照

    • gastrointestinal tissue

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • 纯度

    Purified IgM
  • 纯化说明

    Purified IgM prepared from tissue culture supernatant.
  • 克隆

    单克隆
  • 克隆编号

    NH3
  • 骨髓瘤

    NS1
  • 同种型

    IgM
  • 轻链类型

    unknown
  • 研究领域

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin

相关产品

  • Compatible Secondaries

    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
    • Goat Anti-Mouse IgM H&L (ab9167)
    • Rabbit Anti-Mouse IgM mu chain (ab9175)
  • Isotype control

    • Mouse IgM [B11/7] - Isotype control (ab91545)
  • Related Products

    • F-actin Staining Kit - Red Fluorescence - Cytopainter (ab112127)
    • Phalloidin-iFluor 488 Reagent (ab176753)
    • Phalloidin-iFluor 647 Reagent (ab176759)

靶标

  • 功能

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • 疾病相关

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • 序列相似性

    Belongs to the actin family.
  • 翻译后修饰

    ISGylated.
  • 细胞定位

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P60709 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 60 Human
    • Entrez Gene: 100009272 Rabbit
    • Entrez Gene: 81822 Rat
    • Omim: 102630 Human
    • SwissProt: P60709 Human
    • SwissProt: P29751 Rabbit
    • SwissProt: P60711 Rat
    • Unigene: 520640 Human
    • Unigene: 708120 Human
    • Unigene: 727576 Human
    • Unigene: 94978 Rat
    see all
  • 别名

    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Beta-actin antibody
    • f actin antibody
    • Filamentous actin antibody
    see all

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (79)

发表研究结果有使用 ab205?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab205 被引用在 79 文献中.

  • Zhang Q  et al. Long noncoding RNA MAGI1-IT1 regulates cardiac hypertrophy by modulating miR-302e/DKK1/Wnt/beta-catenin signaling pathway. J Cell Physiol 235:245-253 (2020). PubMed: 31222747
  • Zheng Z  et al. NR4A1 promotes TNF-a-induced chondrocyte death and migration injury via activating the AMPK/Drp1/mitochondrial fission pathway. Int J Mol Med 45:151-161 (2020). PubMed: 31746366
  • Zhao Y  et al. AQP1 suppression by ATF4 triggers trabecular meshwork tissue remodelling in ET-1-induced POAG. J Cell Mol Med 24:3469-3480 (2020). PubMed: 32052937
  • Zhang X  et al. miR-31 Modulates Liver Cancer HepG2 Cell Apoptosis and Invasion via ROCK1/F-Actin Pathways. Onco Targets Ther 13:877-888 (2020). PubMed: 32099392
  • Ye D  et al. Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus. Stem Cell Res Ther 11:382 (2020). PubMed: 32894200
View all Publications for this product

客户评价及客户问答

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提交评价 提交问题

1-10 of 36 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-F-actin antibody [NH3]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - NP40
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

提交于 May 29 2019

Western blot abreview for Anti-F-actin antibody [NH3]

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
U2OS
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

提交于 Apr 05 2019

Immunohistochemistry (Frozen sections) abreview for Anti-F-actin antibody [NH3]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue (Pancreas PanIN)
Permeabilization
No
Specification
Pancreas PanIN
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Methanol
Read More

Abcam user community

Verified customer

提交于 Jun 27 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-F-actin antibody [NH3]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Derived Breast cancer Cell Line)
Permeabilization
Yes - 0.5 % Triton
Specification
Mouse Derived Breast cancer Cell Line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 26°C
Fixative
Methanol
Read More

Abcam user community

Verified customer

提交于 Aug 17 2017

Western blot abreview for Anti-F-actin antibody [NH3]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Derived Breast Cancer Primary Cell Line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
40 µg
Specification
Mouse Derived Breast Cancer Primary Cell Line
Blocking step
Licor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Read More

Abcam user community

Verified customer

提交于 Aug 14 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-F-actin antibody [NH3]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Cortical Neurons)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Cortical Neurons
Blocking step
casein solution + Tween 20 as blocking agent for 20 minute(s) · Concentration: 0.2% · Temperature: 25°C
Fixative
Ethanol
Read More

Abcam user community

Verified customer

提交于 Aug 04 2016

Question

Je suis à la recherche d’un anticorps spécifique de l’actine F et un autre spécifique de l’actine monomérique ou actin-G.

J’aimerais un anticorps déjà testé en Immunofluorescence et pas de toxine type phalloidin.

Read More

Abcam community

Verified customer

Asked on Sep 01 2014

Answer

Je peux vous proposer ab205, Anti-F-actin antibody [NH3] qui est un monoclonal de souris garanti de fonctionner en immunofluorescence. Cependant, nous n’avons pas testé le taux de toxine dans ce produit et donc nous ne pouvons pas garantir qu’il n’y a pas de toxine type phalloidin présent.


Malheureusement, nous n’avons pas d’anticorps anti-G-actine. Tous nos autres anticorps reconnaissent les deux formes F et G.

Read More

Elisa Thomas

Abcam Scientific Support

回复于 Sep 01 2014

Western blot abreview for Anti-F-actin antibody [NH3]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Tissue lysate - whole (CNS)
Specification
CNS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Aug 01 2014

Immunohistochemistry (Resin sections) abreview for Anti-F-actin antibody [NH3]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Resin sections)
Sample
Mouse Tissue sections (Bone Tissue Cells)
Specification
Bone Tissue Cells
Read More

Abcam user community

Verified customer

提交于 Jul 10 2013

Question

I got the reply from the customer in regards to ab205:
"I have tried, according to your suggestion, to do the IHC experiment again with a different secondary antibody "Alexa Fluor® 488 Goat Anti-Mouse IgG, IgM (H+L)" that recognize both IgG and IgM. This didn't recognize the F-actin antibody and resulted in no positive-specific staining.
At this point I think I have tried almost every step in the attempts to get a positive signal."

Looking forward to your reply.
Thanks in advance for your assistance.

Best Regards,

Read More

Abcam community

Verified customer

Asked on Dec 04 2012

Answer

Thank you for your message and for providing this further information.
I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrange a free of charge replacement or refund in compensation.
If you decide to get a free of charge replacement we would recommend it would be beneficial to include a positive control, such as gastrointestinal tissue or brain tissue in the following experiments.
I look forward hearing from you with details of how you would like to proceed.

Read More

Abcam Scientific Support

回复于 Dec 04 2012

1-10 of 36 Abreviews or Q&A

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