Ezrin overexpression 293T裂解物(whole cell) (ab94091)
概述
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产品名称
Ezrin overexpression 293T裂解物(whole cell)
参阅全部 Ezrin 细胞裂解液 -
常规说明
ab94091 is a 293T cell transfected lysate in which Human Ezrin has been transiently over-expressed using a pCMV-Ezrin. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究领域
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背景
Function: Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. Tissue specificity: Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied. Similarity: Contains 1 FERM domain. Developmental stage: Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain. PTM: Phosphorylated by tyrosine-protein kinases.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94091于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution.
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说明 |
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WB
Use at an assay dependent dilution. |
图片
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ab94091 at 15µg/lane on an SDS-PAGE gel.
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All lanes : Anti-Ezrin antibody (ab76872) at 1/500 dilution
Lane 1 :Ezrin overexpression 293T lysate (whole cell) (ab94091)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab94091 尚未被引用在任何文献中。