存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab93865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000. Predicted molecular weight: 70 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. May be involved in development of the eye.
组织特异性Highly expressed in heart and skeletal muscle.
疾病相关Defects in EYA4 are the cause of deafness autosomal dominant type 10 (DFNA10) [MIM:601316]. DFNA10 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Defects in EYA4 are the cause of cardiomyopathy dilated type 1J (CMD1J) [MIM:605362]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
序列相似性Belongs to the HAD-like hydrolase superfamily. EYA family.
- Information by UniProt
- CMD1J antibody
- Deafness, autosomal dominant 10 antibody
- DFNA 10 antibody
All lanes : Anti-EYA4 antibody (ab93865) at 0.4 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 70 kDa
Exposure time: 3 minutes
3µg ab93865 were used to immunoprecipitate EYA4 from 1mg HeLa whole cell lysate. 20% IP was loaded and probed with 1µg/ml ab93865.
Detection: chemiluminescence with exposure time of 10 seconds.
ab93865 has not yet been referenced specifically in any publications.