Anti-ETFB抗体(ab232815)
Key features and details
- Rabbit polyclonal to ETFB
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
概述
-
产品名称
Anti-ETFB抗体
参阅全部 ETFB 一抗 -
描述
兔多克隆抗体to ETFB -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human, Pig
预测可用于: Mouse, Rat, Cow, Orangutan -
免疫原
Recombinant full length protein corresponding to Human ETFB aa 1 to the C-terminus. (Expressed in E.coli).
Database link: P38117 -
阳性对照
- IHC-P: Human liver, breast cancer, prostate cancer, liver cancer, prostate and colorectal cancer tissues. WB: Pig liver, heart and skeletal muscle tissue lysates; Recombinant human ETFB protein.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
ab232815 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab232815于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 28 kDa.
|
|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
说明 |
---|
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 28 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
靶标
-
功能
The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). -
组织特异性
Abundant in liver, heart and skeletal muscle. A weak expression is seen in the brain, placenta, lung, kidney and pancreas. -
疾病相关
Glutaric aciduria 2B -
序列相似性
Belongs to the ETF beta-subunit/FixA family. -
结构域
The recognition loop recognizes a hydrophobic patch at the surface of interacting dehydrogenases and acts as a static anchor at the interface. -
翻译后修饰
Methylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from Acyl-CoA dehydrogenases. -
细胞定位
Mitochondrion matrix. - Information by UniProt
-
数据库链接
- Entrez Gene: 617210 Cow
- Entrez Gene: 2109 Human
- Entrez Gene: 110826 Mouse
- Entrez Gene: 100171463 Orangutan
- Entrez Gene: 396614 Pig
- Entrez Gene: 292845 Rat
- Omim: 130410 Human
- SwissProt: Q2TBV3 Cow
see all -
别名
- Beta ETF antibody
- Beta-ETF antibody
- Electron transfer flavoprotein beta polypeptide antibody
see all
图片
-
Paraffin-embedded human liver tissue stained for ETFB using ab232815 at 20 µg/ml in immunohistochemical analysis. DAB staining.
-
Paraffin-embedded human breast cancer tissue stained for ETFB using ab232815 at 20 µg/ml in immunohistochemical analysis. DAB staining.
-
Paraffin-embedded human prostate cancer tissue stained for ETFB using ab232815 at 20 µg/ml in immunohistochemical analysis. DAB staining.
-
Paraffin-embedded human liver cancer tissue stained for ETFB using ab232815 at 20 µg/ml in immunohistochemical analysis. DAB staining.
-
Paraffin-embedded human prostate tissue stained for ETFB using ab232815 at 20 µg/ml in immunohistochemical analysis. DAB staining.
-
Paraffin-embedded human colorectal cancer tissue stained for ETFB using ab232815 at 20 µg/ml in immunohistochemical analysis. DAB staining.
-
Anti-ETFB antibody (ab232815) at 3 µg/ml + Recombinant human ETFB protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 28 kDa -
All lanes : Anti-ETFB antibody (ab232815) at 3 µg/ml
Lane 1 : Pig liver lysate
Lane 2 : Pig heart lysate
Lane 3 : Pig skeletal muscle lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 28 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab232815 尚未被引用在任何文献中。