参阅全部 ETFA 一抗
描述小鼠单克隆抗体[2B11AE8] to ETFA
经测试应用适用于: IP, WB, ICC/IF, In-Cell ELISA, Flow Cyt, IHC-Pmore details
种属反应性与反应: Mouse, Rat, Human
Human recombinant full length protein ETFA
- HeLa cells Human heart tissue HepG2 whole cells Human liver mitochondria Bovine heart mitochondria Rat liver mitochondria Mouse liver mitochondria
存放说明Shipped at 4°C. Store at +4°C. Do Not Freeze.
存储溶液Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
纯度>95% by SDS-PAGE
纯化说明The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab110316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.|
|ICC/IF||Use a concentration of 4 µg/ml.|
|In-Cell ELISA||Use a concentration of 4 µg/ml.|
|Flow Cyt||Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|IHC-P||Use a concentration of 5 µg/ml.|
功能The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
疾病相关Glutaric aciduria 2A
序列相似性Belongs to the ETF alpha-subunit/FixB family.
结构域Domain I shares an identical polypeptide fold with the beta subunit ETFB though there is no sequence similarity.
翻译后修饰The N-terminus is blocked.
- Information by UniProt
- Alpha ETF antibody
- Alpha-ETF antibody
- Electron transfer flavoprotein alpha polypeptide antibody
Immunocytochemistry image of stained HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the antibody (ab110316, 4 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix
All lanes : Anti-ETFA antibody [2B11AE8] (ab110316) at 1 µg/ml
Lane 1 : Human heart tissue
Lane 2 : HepG2 whole cells
Lane 3 : Human liver mitochondria
Lane 4 : Bovine heart mitochondria
Lane 5 : Rat liver mitochondria
Lane 6 : Mouse liver mitochondria
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDa
HL-60 cells were stained with 1 µg/mL ab110316 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
IHC image of ETFA staining in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110316, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Davoudi M et al. Complex I function and supercomplex formation are preserved in liver mitochondria despite progressive complex III deficiency. PLoS One 9:e86767 (2014). WB ; Mouse . Read more (PubMed: 24466228) »
- Pougovkina O et al. Mitochondrial protein acetylation is driven by acetyl-CoA from fatty acid oxidation. Hum Mol Genet N/A:N/A (2014). Mouse . Read more (PubMed: 24516071) »