The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 18179891
Use a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Use at an assay dependent concentration.
1/50 - 1/200.
Nuclear receptor, may regulate ESR1 transcriptional activity.
Defects in ESRRB are the cause of deafness autosomal recessive type 35 (DFNB35) [MIM:608565]. DFNB35 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Belongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain.
Nuclear receptor subfamily 3 group B member 2 antibody
Orphan nuclear receptor antibody
Steroid hormone receptor ERR 2 antibody
Steroid hormone receptor ERR2 antibody
Western blot - Anti-Estrogen Related Receptor beta antibody (ab19331)
Western blot detection of Estrogen Related Receptor beta from 100 ug of rat kidney lysate (1:500).
Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Related Receptor beta antibody (ab19331)This image is courtesy of an anonymous abreviewer.
ab19331 staining Estrogen Related Receptor Beta protein on mouse embryonic stem cell by ICC/IF. The cells were fixed in 1:1 methanol:acetone and blocked in 0.1% BSA plus 1% serum, for 30 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the cells for 2 hours at 21°C. An Alexa Fluor® 488 conjugated goat anti-rabbit IgG, diluted 1/200, was used as the secondary antibody.
Pacwa A et al. Interplay between estrogen-related receptors and steroidogenesis-controlling molecules in adrenals. In vivo and in vitro study. Acta Histochem120:456-467 (2018).
Read more (PubMed: 29778238) »
Chaudhary S et al. Overexpression of caspase 7 is ERa dependent to affect proliferation and cell growth in breast cancer cells by targeting p21(Cip). Oncogenesis5:e219 (2016).
Read more (PubMed: 27089142) »