Anti-Estrogen Receptor beta抗体(ab3576)
Key features and details
- Rabbit polyclonal to Estrogen Receptor beta
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Estrogen Receptor beta抗体
参阅全部 Estrogen Receptor beta 一抗 -
描述
兔多克隆抗体to Estrogen Receptor beta -
宿主
Rabbit -
特异性
By Western blot, this antibody detects an ~55 kDa protein representing ER beta from Rat brain homogenate. Immunocytochemical staining of ER beta in cells overexpressing the Human protein results in nuclear staining. -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Human
预测可用于: Hamster
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免疫原
Synthetic peptide corresponding to Rat Estrogen Receptor beta aa 467-485.
Sequence:CSSTEDSKNKESSQNLQSQ
(Peptide available asab5018) -
阳性对照
- WB: MCF7 whole cell lysate; T47and SK-BR3 nuclear extracts
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
1/100 - 1/1000.
|
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| WB | (2) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa.
|
| 说明 |
|---|
|
ICC
1/100 - 1/1000. |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa. |
靶标
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功能
Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. -
组织特异性
Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain. -
序列相似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2100 Human
- Omim: 601663 Human
- SwissProt: Q92731 Human
- Unigene: 660607 Human
- Unigene: 729020 Human
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别名
- ER BETA antibody
- ER-beta antibody
- Erb antibody
see all
图片
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Western blot - Anti-Estrogen Receptor beta antibody (ab3576)All lanes : Anti-Estrogen Receptor beta antibody (ab3576) at 1/1000 dilution
Lane 1 : MCF7 whole cell lysates
Lane 2 : MCF7 nuclear extract
Lane 3 : T47D whole cell lysates
Lane 4 : T47D nuclear extract
Lane 5 : SK-BR-3 whole cell lysates
Lane 6 : SK-BR-3 nuclear extract
Lane 7 : Hs 578T whole cell lysates
Lane 8 : Hs 578T nuclear extract
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/40000 dilution
Predicted band size: 59 kDaHDAC1 polyclonal antibody (bottom panel) at a dilution of 1/1000 overnight at 4C, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/40,000. Chemiluminescent detection.
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Immunocytochemistry - Anti-Estrogen Receptor beta antibody (ab3576)Immunofluorescent analysis using Estrogen Receptor beta polyclonal antibody (ab3576) shows nuclear localization of Estrogen Receptor beta in MCF7 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing Estrogen Receptor beta (ab3576), at a dilution of 1:100 for 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
数据表及文件
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SDS download
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Datasheet download
文献 (81)
ab3576 被引用在 81 文献中.
- Meng Q et al. Estrogen prevent atherosclerosis by attenuating endothelial cell pyroptosis via activation of estrogen receptor a-mediated autophagy. J Adv Res 28:149-164 (2021). PubMed: 33364052
- Hexiao T et al. Knockdown of CENPF inhibits the progression of lung adenocarcinoma mediated by ERß2/5 pathway. Aging (Albany NY) 13:2604-2625 (2021). PubMed: 33428600
- Liu J et al. Maternal Diabetes-Induced Suppression of Oxytocin Receptor Contributes to Social Deficits in Offspring. Front Neurosci 15:634781 (2021). PubMed: 33633538
- Zeng M et al. In vitro Non-Small Cell Lung Cancer Inhibitory Effect by New Diphenylethane Isolated From Stems and Leaves of Dioscorea oppositifolia L. via ERß-STAT3 Pathway. Front Pharmacol 12:622681 (2021). PubMed: 33708130
- Blum KM et al. Sex and Tamoxifen confound murine experimental studies in cardiovascular tissue engineering. Sci Rep 11:8037 (2021). PubMed: 33850181