- 数据表
- 具体的参考文献 (31)
- 实验方案
概述
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产品名称Anti-Estrogen Receptor beta抗体
参阅全部 Estrogen Receptor beta 一抗 -
描述兔多克隆抗体to Estrogen Receptor beta
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宿主Rabbit
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特异性By Western blot, this antibody detects an ~55 kDa protein representing ER beta from Rat brain homogenate. Immunocytochemical staining of ER beta in cells overexpressing the Human protein results in nuclear staining.
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经测试应用适用于: IHC-P, IHC-Fr, ICC, WB, Inhibition Assay, ICC/IFmore details
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种属反应性与反应: Mouse, Rat, Sheep, Human, Pig, Non human primates
预测可用于: Hamster
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免疫原
Synthetic peptide corresponding to Rat Estrogen Receptor beta aa 467-485.
Sequence:CSSTEDSKNKESSQNLQSQ
(Peptide available asab5018) -
阳性对照
- WB: Rat brain lysate IHC: testis, efferent ductules, epididymis, and vas deferens (see Zhou et al), Rat ovary (see Sanchez-Criado)
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Immunohistochemistry kits
应用
Our Abpromise guarantee covers the use of ab3576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/50. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. | |
| IHC-Fr | 1/50. | |
| ICC | 1/100 - 1/1000. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).Can be blocked with Estrogen Receptor beta peptide (ab5018). | |
| Inhibition Assay | Use at an assay dependent concentration. | |
| ICC/IF | 1/100 - 1/1000. |
靶标
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功能Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
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组织特异性Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
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序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
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细胞定位Nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 2100 Human
- Entrez Gene: 13983 Mouse
- Entrez Gene: 396697 Pig
- Entrez Gene: 25149 Rat
- Omim: 601663 Human
- SwissProt: Q92731 Human
- SwissProt: O08537 Mouse
- SwissProt: Q9XSW2 Pig
see all -
别名
- ER BETA antibody
- ER-beta antibody
- Erb antibody
see all
图片
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Immunohistochemistry (Frozen sections) - Anti-Estrogen Receptor beta antibody (ab3576)Image from Novensà L et al., PLoS One. 2011;6(9):e25335. Epub 2011 Sep 22. Fig 4.; doi:10.1371/journal.pone.0025335; PLoS ONE 6(9): e25335.Immunofluorescence analysis of frozen murine thoracic aorta, staining Estrogen Receptor beta with ab3576 at 1/50 dilution (green: not merged with nucleus, or purple: merged with nucleus). Nuclei were stained with DAPI (blue) and actin fibers were stained red. -
Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor beta antibody (ab3576)Immunofluorescent analysis using Estrogen Receptor beta polyclonal antibody (ab3576) shows nuclear localization of Estrogen Receptor beta in MCF7 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing Estrogen Receptor beta (ab3576), at a dilution of 1:100 for 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification. -
Western blot - Anti-Estrogen Receptor beta antibody (ab3576)All lanes : Anti-Estrogen Receptor beta antibody (ab3576) at 1/2000 dilution
Lane 1 : CAL region of Human brain - Whole cell lysate
Lane 2 : EC region of Human brain - Whole cell lysate
Lane 3 : HIP region of Human brain - Whole cell lysate
Lane 4 : IT region of Human brain - Whole cell lysate
Lane 5 : MF region of Human brain - Whole cell lysate
Lane 6 : SP region of Human brain - Whole cell lysate
Secondary
All lanes : HRP conjugated Donkey Anti-rabbit IgG
Predicted band size: 59 kDa
Observed band size: 55 kDa (why is the actual band size different from the predicted?)
This image is courtesy of an Abreview submitted on 3 February 2006. -
Immunocytochemistry - Anti-Estrogen Receptor beta antibody (ab3576)Immunolocalization of ER beta from CHO cells overexpressing the Human protein, using ab3576 (1ug/ml).
实验方案
文献
This product has been referenced in:
- Yan L et al. Effects of Ovariectomy in an hSOD1-G93A Transgenic Mouse Model of Amyotrophic Lateral Sclerosis (ALS). Med Sci Monit 24:678-686 (2018). Read more (PubMed: 29394243) »
- Seo SY et al. An estradiol-independent BDNF-NPY cascade is involved in the antidepressant effect of mechanical acupuncture instruments in ovariectomized rats. Sci Rep 8:5849 (2018). Read more (PubMed: 29643431) »
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