Name: Anti-Estrogen Receptor beta antibody [14C8]
SKU: ab288
Rating: 4 (9 reviews)

产品名称

Anti-Estrogen Receptor beta抗体[14C8]
参阅全部 Estrogen Receptor beta 一抗
描述

小鼠单克隆抗体[14C8] to Estrogen Receptor beta
宿主

Mouse
特异性

14C8 is specific for human ER-beta. The antibody has been tested in WB against recombinant protein. It has not yet been tested in our laboratory using any cell lysates and several publications suggest it can give weak bands in breast tumour extracts lysate. Strong Erb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumors using the same antibody.
经测试应用

适用于: WB, Flow Cyt, IHC-P, ICC/IF, ChIP, IHC-Frmore details
种属反应性

与反应: Mouse, Human, Monkey, Baboon
免疫原

Recombinant 6-His fusion protein encoding amino acids 1-153 of human ER-beta expressed in E. coli.
阳性对照
- WB: Recombinant fusion protein. IHC-P: endocervical epithelium, Lobular breast carcinoma.
常规说明

This product was changed from ascites to tissue culture supernatant on 23^rd October 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

形式

Liquid
存放说明

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液

pH: 7.40
Constituent: 0.0268% PBS
Concentration information loading...
纯度

Immunogen affinity purified
纯化说明

Purified from TCS
克隆

单克隆
克隆编号

14C8
骨髓瘤

NS1
同种型

IgG2b
轻链类型

kappa
研究领域

ChIP Related Products
- ChIP Kit (ab500)
Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit (ab102918)
- APC Conjugation Kit (ab201807)
Immunohistochemistry kits
- Mouse on Mouse Polymer IHC Kit (ab127055)
Isotype control
- Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)

Our Abpromise guarantee covers the use of ab288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用	Ab评论	说明
WB		Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 59 kDa.
Flow Cyt		1/25. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-P		Use a concentration of 5 µg/ml.
ICC/IF		Use at an assay dependent concentration.
ChIP		Use at an assay dependent concentration.
IHC-Fr		Use at an assay dependent concentration.

功能

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
组织特异性

Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
序列相似性

Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
结构域

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
细胞定位

Nucleus.
Target information above from: UniProt accession Q92731 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 2100 Human
- Entrez Gene: 13983 Mouse
- Omim: 601663 Human
- SwissProt: Q92731 Human
- SwissProt: O08537 Mouse
- Unigene: 660607 Human
- Unigene: 729020 Human
- Unigene: 2561 Mouse
别名
- ER BETA antibody
- ER-beta antibody
- Erb antibody
- ESR B antibody
- ESR BETA antibody
- ESR2 antibody
- ESR2_HUMAN antibody
- ESRB antibody
- ESTRB antibody
- estrogen nuclear receptor beta variant a antibody
- estrogen nuclear receptor beta variant b antibody
- estrogen receptor 2 (ER beta) antibody
- Estrogen receptor 2 antibody
- estrogen receptor beta 4 antibody
- Estrogen receptor beta antibody
- NR3A2 antibody
- Nuclear receptor subfamily 3 group A member 2 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta antibody [14C8] (ab288)

ab288 staining endocervical epithelium by IHC-P.
Western blot - Anti-Estrogen Receptor beta antibody [14C8] (ab288)

All lanes : Anti-Estrogen Receptor beta antibody [14C8] (ab288) at 1/5000 dilution

Lane 1 : Non-transfected 293T whole cell extracts
Lane 2 : Transfected 293T whole cell extracts

Lysates/proteins at 30 µg per lane.

Performed under non-reducing conditions.

Predicted band size: 59 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta antibody [14C8] (ab288)

ab288 staining infiltrating lobular carcinoma of the breast by IHC-P.

ChIP protocols
Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

This product has been referenced in:

Tang YQ et al. Fructus Ligustri Lucidi modulates estrogen receptor expression with no uterotrophic effect in ovariectomized rats. BMC Complement Altern Med 18:118 (2018). Read more (PubMed: 29609586) »
Alexander BH et al. Stable Density and Dynamics of Dendritic Spines of Cortical Neurons Across the Estrous Cycle While Expressing Differential Levels of Sensory-Evoked Plasticity. Front Mol Neurosci 11:83 (2018). Read more (PubMed: 29615867) »

See all 62 Publications for this product

发表研究结果有使用 ab288？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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1-10 of 14 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (MDA231 tumor taken out of Balb/C Nude mice)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate pH6

Permeabilization

Specification

MDA231 tumor taken out of Balb/C Nude mice

Blocking step

Innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C

Fixative

Formaldehyde

Abcam user community

Verified customer

提交于 Nov 28 2018

Application

Western blot

Sample

Human Cell lysate - whole cell (MDA-MB-231 cells)

Gel Running Conditions

Reduced Denaturing (4-20% Tris-Glycin gels)

Loading amount

50 µg

Specification

MDA-MB-231 cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Nov 28 2018

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (OV90)

Permeabilization

Yes - 0.25% Triton-X100 in PBS

Specification

OV90

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Fixative

Formaldehyde

Abcam user community

Verified customer

提交于 Oct 26 2016

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate, pH 6.0

Sample

Baboon Tissue sections (fetal ovary)

Specification

fetal ovary

Permeabilization

Fixative

Formaldehyde

Ms. Marcia Burch

Verified customer

提交于 Sep 25 2014

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Breast cancer)

Specification

Breast cancer

Fixative

Paraformaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: TRIS-EDTA-Buffer pH 9,0

Permeabilization

Yes - Wash Buffer with tween

Mr. Rudolf Jung

Verified customer

提交于 Dec 03 2012

Question

Dear sir, madam,

There is some literature stating that storage of tisse slides reduces antigen detection particulary for IHC and particulary for estrogen receptor. According to a recent article the main factor explaning the reduced staining intensity over time is the primary antibody used (Karlsson. Effects of long-term storage on the detection of proteins, DNA and mRNA in tissue microarray slides. J Histochem Cytochem 2011).

Do you have any experience about this topic related to Ab288? I just ordered two.

Thank you very much in advance.

Best regards,

Abcam community

Verified customer

Asked on Aug 15 2012

Answer

Thank you for your enquiry and your interest in our products.
The effect of formalin fixation and the long-term storage on the detectability of different proteins have been studies for some time. The longer the tissues are fixed and stored in formalin, less intensive staining can be obtained due to epitope accessibility and/or protein degradation. Generally, the optimal time for formalin fixation for most stains is around 3-7 days.
Currently, we do not have specific data/publications on ab288 regarding this issue.
If you need any further assistance in the future, please do not hesitate to contact me.

Abcam Scientific Support

回复于 Aug 15 2012

Question

LOT NUMBER GR60665-3 ORDER NUMBER 1004022 DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE whole cell lysate, human MCF-7, Keratinocyte PRIMARY ANTIBODY Concentration or dilution: 1/1000, 1/500, 1/200 Diluent buffer : 5% skim milk Incubation time : overnight Incubation temperature: 4℃ Wash Buffer : 1x TBST Number of washes : 3 times DETECTION METHOD ECL ANTIBODY STORAGE CONDITIONS -20C SAMPLE PREPARATION Lysis buffer : RIPA buffer Protease inhibitors: yes Phosphatase inhibitors Reducing agent : SDS and betaME Boiling for ≥5 min? yes AMOUNT OF PROTEIN LOADED 30 and 50ug/well ELECTROPHORESIS/GEL CONDITIONS 10% SDS-PAGE gel TRANSFER AND BLOCKING CONDITIONS Type of membrane : nitrocellulose Protein transfer verified: protein transfer was verified with ponceau S solution Blocking agent and concentration: 5% skim milk in 1xTBST Blocking time : 30min. Blocking temperature : room temperature SECONDARY ANTIBODY Concentration or dilution: 1/5000 Diluent buffer : 5% skim milk Incubation time : 2h Incubation temperature: room temperature Wash Buffer : 1x TBST Number of washes : 3 times HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? I tried to change the concentration of primary antibody and protein loading amount

Abcam community

Verified customer

Asked on Jan 09 2012

Answer

Thank you for taking time to liaise with this customer to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab288. I would also appreciate if you can confirm some further details: 1.) The MCF-7 cells do express low levels of the estrogen receptor beta (http://www.ncbi.nlm.nih.gov/pubmed/10601611, https://www.abcam.com/index.html?datasheet=288&tab=abreviews&intabreviewid=7400where it has been induced) and as far as I know, the expression in keratinocytes can also be variable. Does the customer know that the samples used do express high enough levels of Estrogen Receptor beta? If not, I can suggest to use another positive control, such as mouse brain extractor PC12 cell lysate for example. Does the customer have such asample? 2.) Secondly, I can also recommend touse BSA as a blocking buffer. Indeed, changing the blockingbuffercan significantly improve the results. Please seethe western blot image on the datasheet of ab9385 for an illustration. https://www.abcam.com/index.html?datasheet=9385 (or use the following: https://www.abcam.com/index.html?datasheet=9385). 3.) I am not sure how the primary antibody incubation has been performed. I canhowever recommend to incubate the primary antibody overnight at 4 degrees Celsius. Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. I hope this information is helpful, and I thank you for your cooperation.

Abcam Scientific Support

回复于 Jan 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (vaginal)

Specification

vaginal

Fixative

Paraformaldehyde

Permeabilization

Yes - 0.2% Triton in PBS

Blocking step

(agent) for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Oct 17 2011

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (breast cancer)

Specification

breast cancer

Fixative

Paraformaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: • Microwave slides in 10mM citrate buffer pH 6.0

Permeabilization

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Dr. W Xu

Verified customer

提交于 May 11 2010

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Human Cell lysate - whole cell (MCF-7 cells)

Loading amount

50 µg

Specification

MCF-7 cells

Treatment

Doxycycline for 48 hrs

Blocking step

Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%

Dr. George Skliris

Verified customer

提交于 May 09 2007

1-10 of 14 Abreviews or Q&A

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

定制方案及合作关系

Anti-Estrogen Receptor beta抗体[14C8] (ab288)

概述

性能

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This product has been referenced in:

客户评价及客户问答

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor beta antibody [14C8]

Western blot abreview for Anti-Estrogen Receptor beta antibody [14C8]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Estrogen Receptor beta antibody [14C8]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor beta antibody [14C8]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor beta antibody [14C8]

Immunohistochemistry (Frozen sections) abreview for Anti-Estrogen Receptor beta antibody [14C8]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor beta antibody [14C8]

Western blot abreview for Anti-Estrogen Receptor beta antibody [14C8]