Key features and details
- Mouse monoclonal [14C8] to Estrogen Receptor beta
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Monkey
- Isotype: IgG2b
产品名称Anti-Estrogen Receptor beta抗体[14C8]
参阅全部 Estrogen Receptor beta 一抗
描述小鼠单克隆抗体[14C8] to Estrogen Receptor beta
经测试应用适用于: WB, IHC-Pmore details
种属反应性与反应: Mouse, Human, Monkey
Recombinant fragment corresponding to Human Estrogen Receptor beta aa 1-153. Expressed in E.coli.
Database link: Q92731
- WB: Recombinant fusion protein, human liver and intestine, HEK-293T cells. IHC-P: Endocervical epithelium, Lobular breast carcinoma and human breast carcinoma.
This product was changed from ascites to tissue culture supernatant on 23rd October 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 100% PBS
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified from TCS
ChIP Related Products
Our Abpromise guarantee covers the use of ab288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 59 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
功能Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
组织特异性Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
- Information by UniProt
- ER BETA antibody
- ER-beta antibody
- Erb antibody
Paraffin embedded human breast carcinoma tissue stained for Estrogen Receptor beta using ab288 at 1/200 dilution in immunohistochemical analysis.
All lanes : Anti-Estrogen Receptor beta antibody [14C8] (ab288) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T whole cell extracts
Lane 2 : Transfected HEK-293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa
ab288 staining Estrogen Receptor beta in infiltrating lobular carcinoma of the human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde. Endogenous peroxidase blocking was with 3% H2O2 at room temperature for 30 minutes. Blocking with 1.5% goat serum (dilute goat serum by 1xPBS), at room temperature for 30min; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0, 15min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Samples were incubated with primary antibody (1/100) for overnight at 4°C. An ABC HRP Kit Mouse IgG) was used as the secondary antibody (1/200), room temperature for 30min. Washed twice for 5 minutes using PBS. DAB was used as a substrate.
All lanes : Anti-Estrogen Receptor beta antibody [14C8] (ab288) at 1/500 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human liver tissue lysate
Lane 3 : Human intestine tissue lysate
Lysates/proteins at 30 µg per lane.
All lanes : Mouse IgG antibody (HRP) at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 59 kDa
Running condition: 80V, 15min; 140V, 40min.
Transfer condition: Semi-dry, 18 V, 60min (NC membrane)
blocking condition: 5% non-fat milk in TBST, RT, 60min.
primary antibody incubation: 4°C overnight.
Secondary antibody incubation: room temperature for 1 hour.
Washing conditions: 5 ml TBST, 4 x 5min.
ab288 staining endocervical epithelium by IHC-P.
ab288 被引用在 79 文献中.
- Gu J et al. Targeting the ERß/Angiopoietin-2/Tie-2 signaling-mediated angiogenesis with the FDA-approved anti-estrogen Faslodex to increase the Sunitinib sensitivity in RCC. Cell Death Dis 11:367 (2020). PubMed: 32409702
- Li H et al. CLPTM1L induces estrogen receptor ß signaling-mediated radioresistance in non-small cell lung cancer cells. Cell Commun Signal 18:152 (2020). PubMed: 32943060
- Song P et al. Estrogen receptor ß inhibits breast cancer cells migration and invasion through CLDN6-mediated autophagy. J Exp Clin Cancer Res 38:354 (2019). PubMed: 31412908
- Datta N et al. Promyelocytic Leukemia (PML) gene regulation: implication towards curbing oncogenesis. Cell Death Dis 10:656 (2019). PubMed: 31506431
- López-Romero R et al. The expression of transcription factor BORIS and its association with the estrogen receptor beta (ER-ß) in cervical carcinogenesis. Int J Clin Exp Pathol 12:3208-3221 (2019). PubMed: 31934165