Anti-Estrogen Receptor beta抗体[14C8] (ab288)
- 数据表
- 具体的参考文献 (59)
- 实验方案
概述
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产品名称Anti-Estrogen Receptor beta抗体[14C8]
参阅全部 Estrogen Receptor beta 一抗 -
描述小鼠单克隆抗体[14C8] to Estrogen Receptor beta
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宿主Mouse
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特异性14C8 is specific for human ER-beta. The antibody has been tested in WB against recombinant protein. It has not yet been tested in our laboratory using any cell lysates and several publications suggest it can give weak bands in breast tumour extracts lysate. Strong Erb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumors using the same antibody.
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经测试应用适用于: WB, Flow Cyt, IHC-P, ICC/IF, ChIP, IHC-Frmore details
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种属反应性与反应: Mouse, Human, Monkey, Baboon
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免疫原
Recombinant 6-His fusion protein encoding amino acids 1-153 of human ER-beta expressed in E. coli.
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阳性对照
- WB: Recombinant fusion protein. IHC-P: endocervical epithelium, Lobular breast carcinoma.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液pH: 7.40
Constituent: 0.0268% PBS -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆单克隆
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克隆编号14C8
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骨髓瘤NS1
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同种型IgG2b
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轻链类型kappa
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研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
应用
Our Abpromise guarantee covers the use of ab288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 59 kDa. | |
| Flow Cyt | 1/25. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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| IHC-P | Use a concentration of 5 µg/ml. | |
| ICC/IF | Use at an assay dependent concentration. | |
| ChIP | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. |
靶标
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功能Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
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组织特异性Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
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序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
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细胞定位Nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 2100 Human
- Entrez Gene: 13983 Mouse
- Omim: 601663 Human
- SwissProt: Q92731 Human
- SwissProt: O08537 Mouse
- Unigene: 660607 Human
- Unigene: 729020 Human
- Unigene: 2561 Mouse
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别名
- ER BETA antibody
- ER-beta antibody
- Erb antibody
see all
图片
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta antibody [14C8] (ab288)](https://www.abcam.cn/ps/products/0/ab288/Images/ab288_5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta antibody [14C8] (ab288)ab288 staining endocervical epithelium by IHC-P. -
![Western blot - Anti-Estrogen Receptor beta antibody [14C8] (ab288)](https://www.abcam.cn/ps/products/0/ab288/Images/ab288-274249-ab288-ERb-WB-supplier.PNG)
Western blot - Anti-Estrogen Receptor beta antibody [14C8] (ab288)All lanes : Anti-Estrogen Receptor beta antibody [14C8] (ab288) at 1/5000 dilution
Lane 1 : Non-transfected 293T whole cell extracts
Lane 2 : Transfected 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Performed under non-reducing conditions.
Predicted band size: 59 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta antibody [14C8] (ab288)](https://www.abcam.cn/ps/products/0/ab288/Images/ab288_6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta antibody [14C8] (ab288)ab288 staining infiltrating lobular carcinoma of the breast by IHC-P.
实验方案
文献
This product has been referenced in:
- Tang YQ et al. Fructus Ligustri Lucidi modulates estrogen receptor expression with no uterotrophic effect in ovariectomized rats. BMC Complement Altern Med 18:118 (2018). Read more (PubMed: 29609586) »
- Alexander BH et al. Stable Density and Dynamics of Dendritic Spines of Cortical Neurons Across the Estrous Cycle While Expressing Differential Levels of Sensory-Evoked Plasticity. Front Mol Neurosci 11:83 (2018). Read more (PubMed: 29615867) »
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