Anti-Estrogen Receptor beta抗体[14C8] (ab288)

Thank you for your enquiry.

I can confirm that we recommend not to dilute antibodies before storage unless is states to do so on the individual datasheet. For all antibodies, we suggest to always follow instructions provided on the individual datasheets. Suggesting to dilute an antibody before storage would be very rare. The antibodies are more stable at a higher concentration.

- anti-IGFBP1 (ab111203)
From the information provided on the datasheet, size is 100 ug, concentration 1 mg/ml.

With the following common laboratory calculations you can work out the volume:

We provide 100 ug at 1000 ug/ml
Therefore: 1000 ug / 100 ug = 10
Therefore 1 ml / factor of 10 = 100 ul

- Anti estrogen receptor beta antibody 14C8 (ab288)

This is also 100 ul

- anti estrogen receptor alpha antibody 6F11 (ab9269). For this antibody I do not have the concentration. Should I add some buffer to it before using?

I am sorry the concentration for this particular antibody is batch dependent and so without this information I am not able to confirm how much should be in the vial. I would appreciate if you are able to provide the batch number of the vial you have? We recommend not to dilute this antibody before storage. As on the datasheet, store at +4oC short term (1-2 weeks). Aliquot and store at -20oC or -80oC. Avoid repeated freeze / thaw cycles.

- anti progesterone receptor antibody PR-AT 4.14 - ChIP Grade (ab2764)

I am sorry the concentration for this particular antibody is batch dependent and so without this information I am not able to confirm how much should be in the vial. I would appreciate if you are able to provide the batch number of the vial you have?

- anti CD51 + CD61 antibody BV3 (ab7166)

On the datasheet, 50 ug at 0.1 mg/ml

This is 100 ug / 50 = 2
1 ml / factor of 2 = 500 ul

I hope this will be helpful to you. Please do not hesitate to contact me with the requested information if you require further assistance.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.


Please apologize for the delay of my answer: Anja is on her well deserved holiday now, so I am taking over of your case.


I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 123456789. Unfortunately, the antibody will probably be delivered on the 30th of August.


To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for your enquiry and your interest in our products.
The effect of formalin fixation and the long-term storage on the detectability of different proteins have been studies for some time. The longer the tissues are fixed and stored in formalin, less intensive staining can be obtained due to epitope accessibility and/or protein degradation. Generally, the optimal time for formalin fixation for most stains is around 3-7 days.
Currently, we do not have specific data/publications on ab288 regarding this issue.
If you need any further assistance in the future, please do not hesitate to contact me.

Thank you for taking time to liaise with this customer to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab288. I would also appreciate if you can confirm some further details: 1.) The MCF-7 cells do express low levels of the estrogen receptor beta (http://www.ncbi.nlm.nih.gov/pubmed/10601611, https://www.abcam.com/index.html?datasheet=288&tab=abreviews&intabreviewid=7400where it has been induced) and as far as I know, the expression in keratinocytes can also be variable. Does the customer know that the samples used do express high enough levels of Estrogen Receptor beta? If not, I can suggest to use another positive control, such as mouse brain extractor PC12 cell lysate for example. Does the customer have such asample? 2.) Secondly, I can also recommend touse BSA as a blocking buffer. Indeed, changing the blockingbuffercan significantly improve the results. Please seethe western blot image on the datasheet of ab9385 for an illustration. https://www.abcam.com/index.html?datasheet=9385 (or use the following: https://www.abcam.com/index.html?datasheet=9385). 3.) I am not sure how the primary antibody incubation has been performed. I canhowever recommend to incubate the primary antibody overnight at 4 degrees Celsius. Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. I hope this information is helpful, and I thank you for your cooperation.

Thank you for your inquiry. The 6x his tag was not removed before the antibody was raised. If I can help provide any further information please let me know.