重组Anti-Estrogen Receptor alpha抗体[SP1] (ab16660)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP1] to Estrogen Receptor alpha
- Suitable for: mIHC, Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Estrogen Receptor alpha抗体[SP1]
参阅全部 Estrogen Receptor alpha 一抗 -
描述
兔单克隆抗体[SP1] to Estrogen Receptor alpha -
宿主
Rabbit -
经测试应用
适用于: mIHC, Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is considered to be commercially sensitive.
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表位
C-terminal -
阳性对照
- WB: MCF7 cell lysate. IHC-P: Human breast carcinoma, cervix, breast, breast ductal carcinoma and ovarian adenocarcinoma tissue. ICC/IF: MCF7 cells. Flow Cyt (intra): MCF7 cells. mIHC: Human triple-positive breast carcinoma, Human mammary gland tissue sections
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常规说明
This product has switched from a hybridoma to recombinant production format on 21st May 2020.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
SP1 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16660于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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mIHC |
1/200.
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Flow Cyt (Intra) |
1/200.
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WB |
1/25. Predicted molecular weight: 67 kDa.
Incubate for 1 hour at room temperature. |
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IHC-P | (2) |
1/200.
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ICC/IF | (1) |
1/25 - 1/250.
|
说明 |
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mIHC
1/200. |
Flow Cyt (Intra)
1/200. |
WB
1/25. Predicted molecular weight: 67 kDa. Incubate for 1 hour at room temperature. |
IHC-P
1/200. |
ICC/IF
1/25 - 1/250. |
靶标
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功能
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1. -
序列相似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
翻译后修饰
Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
细胞定位
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. - Information by UniProt
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数据库链接
- Entrez Gene: 2099 Human
- Entrez Gene: 13982 Mouse
- Omim: 133430 Human
- SwissProt: P03372 Human
- SwissProt: P19785 Mouse
- Unigene: 208124 Human
- Unigene: 463262 Mouse
- Unigene: 9213 Mouse
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别名
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
see all
图片
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Tissue Microarrays stained for Anti-Estrogen Receptor alpha antibody [SP1] using ab16660 in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negaive (cross mark) staining per sample type tested. The section was incubated with ab16660 for 10 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins.
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Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human triple-positive breast carcinoma tissue sections labeling Estrogen Receptor (ER) with ab16660, at a 1/200 dilution ( 0.07 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins and Opal Polymer HRP Ms + Rb was used as the secondary antibody. DAPI was used as the nuclear counterstain.
Panel A: merged staining of anti-Progesterone Receptor (PR) (magenta; Opal™690), anti-HER2 (red; Opal™570) and anti-Estrogen Receptor (ER) (green; Opal™520) on human triple-positive breast carcinoma.
Panel B: anti-PR stained on nucleus of cancer cells.
Panel C: anti-HER2 stained on membrane of cancer cells.
Panel D: anti-ER stained on nucleus of cancer cells.The section was incubated in three rounds of staining: in the order of ab16661 for 30 mins, then ab16660 and ab231438 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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ab16660 staining Estrogen Receptor alpha in MCF7 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab16660 at 1/250 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This image was generated using the hybridoma version of the product.
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Anti-Estrogen Receptor alpha antibody [SP1] (ab16660) at 1/25 dilution + lysate prepared from MCF7 cells
Predicted band size: 67 kDaThis image was generated using the hybridoma version of the product.
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Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human mammary gland tissue sections labeling Estrogen Receptor (ER) with ab16660, at a 1/200 dilution ( 0.07 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins and Opal Polymer HRP Ms + Rb was used as the secondary antibody. DAPI was used as the nuclear counterstain.
Panel A: merged staining of anti-Progesterone Receptor (PR) (magenta; Opal™690), anti-HER2 (red; Opal™570) and anti-Estrogen Receptor (ER) (green; Opal™520) on human mammary gland.
Panel B: anti-PR stained on nucleus of some ductal cells.
Panel C: anti-HER2 stained on no cells.
Panel D: anti-ER stained on nucleus of some ductal cells.The section was incubated in three rounds of staining: in the order of ab16661 for 30 mins, then ab16660 and ab231438 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human triple-negative breast carcinoma tissue sections labeling Estrogen Receptor (ER) with ab16660, at a 1/200 dilution ( 0.07 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins and Opal Polymer HRP Ms + Rb was used as the secondary antibody. DAPI was used as the nuclear counterstain.
Panel A: merged staining of anti-Progesterone Receptor (PR) (magenta; Opal™690), anti-HER2 (red; Opal™570) and anti-Estrogen Receptor (ER) (green; Opal™520) on human triple-negative breast carcinoma.
Panel B: anti-PR stained on no cells.
Panel C: anti-HER2 stained on no cells.
Panel D: anti-ER stained on no cells.The section was incubated in three rounds of staining: in the order of ab16661 for 30 mins, then ab16660 and ab231438 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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IHC image of ab16660 staining Estrogen Receptor alpha in normal human cervix formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16660, 1/250 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
This image was generated using the hybridoma version of the product.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Estrogen Receptor alpha using ab16660 at 1/200 dilution in immunohistochemical analysis.
This image was generated using the hybridoma version of the product.
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Immunohistochemical analysis of tamoxifen resistant MCF7 xenograft tumours staining estrogen receptor alpha with ab16660. The mice used were treated with a vehicle, tamoxifen (120mg/kg/day p.o) or GDC-0810 (100mg/kg/day p.o.) for 27 days.
This image was generated using the hybridoma version of the product.
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Intracellular flow cytometry analysis of MCF7 (human breast adenocarcinoma epithelial cell) labeling Estrogen Receptor alpha with purified ab16660 at 1/200 dilution (1.06 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue). This image was generated using the hybridoma version of the product.
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Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Estrogen Receptor alpha using ab16660 at 1/200 dilution in immunohistochemical analysis.
This image was generated using the hybridoma version of the product.
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Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling Estrogen Receptor alpha with purified ab16660 at 1/25 (8.5 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This image was generated using the hybridoma version of the product.
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Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Estrogen Receptor alpha using ab16660 at 1/200 dilution in immunohistochemical analysis.
This image was generated using the hybridoma version of the product.
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Formalin-fixed, paraffin-embedded human breast tissue stained for Estrogen Receptor alpha using ab16660 at 1/200 dilution in immunohistochemical analysis.
This image was generated using the hybridoma version of the product.
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Human breast carcinoma stained with ab16660.
This image was generated using the hybridoma version of the product.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (57)
ab16660 被引用在 57 文献中.
- Watabe S et al. Role of Lamin A and emerin in maintaining nuclear morphology in different subtypes of ovarian epithelial cancer. Oncol Lett 23:9 (2022). PubMed: 34820008
- Liu Y et al. Combining Organoid Models with Next-Generation Sequencing to Reveal Tumor Heterogeneity and Predict Therapeutic Response in Breast Cancer. J Oncol 2022:9390912 (2022). PubMed: 36046364
- Mori H et al. Influence of Estrogen Treatment on ESR1+ and ESR1- Cells in ER+ Breast Cancer: Insights from Single-Cell Analysis of Patient-Derived Xenograft Models. Cancers (Basel) 13:N/A (2021). PubMed: 34944995
- Cunha GR et al. Ontogeny of estrogen receptors in human male and female fetal reproductive tracts. Differentiation 118:107-131 (2021). PubMed: 33176961
- Hughbanks ML et al. Estrogen receptor Alpha in human knee articular cartilage of healthy and osteoarthritic females. J Orthop 27:1-8 (2021). PubMed: 34413582