Anti-Estrogen Receptor alpha抗体(ab3575)
Key features and details
- Rabbit polyclonal to Estrogen Receptor alpha
- Suitable for: ICC, IHC-P, WB
- Reacts with: Rat, Human, Recombinant fragment
- Isotype: IgG
重组抗体的批间可重复性更强
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Estrogen Receptor alpha抗体
参阅全部 Estrogen Receptor alpha 一抗 -
描述
兔多克隆抗体to Estrogen Receptor alpha -
宿主
Rabbit -
经测试应用
适用于: ICC, IHC-P, WBmore details -
种属反应性
与反应: Rat, Human, Recombinant fragment
预测可用于: Hamster, Cow, Cat
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免疫原
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阳性对照
- WB: recombinant fragment; IHC: rat prostate tissue; ICC: T47D and 578T breast cancer cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
1/1000 - 1/4000.
|
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| IHC-P | (1) |
Use a concentration of 1 - 5 µg/ml.
|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Estrogen Receptor alpha peptide (ab5847).
|
| 说明 |
|---|
|
ICC
1/1000 - 1/4000. |
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IHC-P
Use a concentration of 1 - 5 µg/ml. |
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Estrogen Receptor alpha peptide (ab5847). |
靶标
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功能
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1. -
序列相似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
翻译后修饰
Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
细胞定位
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. - Information by UniProt
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数据库链接
- Entrez Gene: 407238 Cow
- Entrez Gene: 101825539 Hamster
- Entrez Gene: 2099 Human
- Entrez Gene: 24890 Rat
- Omim: 133430 Human
- SwissProt: P49884 Cow
- SwissProt: Q9QZJ5 Hamster
- SwissProt: P03372 Human
see all -
别名
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab3575)
ab3575 at a dilution of 5 µg/ml staining ER alpha in rat prostate tissue sections.
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Western blot - Anti-Estrogen Receptor alpha antibody (ab3575)All lanes : Anti-Estrogen Receptor alpha antibody (ab3575) at 1/1000 dilution
Lane 1 : Recombinant full-length human Estrogen Receptor alpha protein, 250 ng
Lane 2 : Recombinant full-length human Estrogen Receptor alpha protein, 125 ng
Lane 3 : Recombinant full-length human Estrogen Receptor alpha protein, 62.5 ng
Lane 4 : Recombinant full-length human Estrogen Receptor alpha protein, 31.25 ng
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/40000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDaBlocked with 5% milk in TBST for at least 1 hour at room temperature.
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Immunocytochemistry - Anti-Estrogen Receptor alpha antibody (ab3575)4% paraformaldehyde fixed 0.1% Triton permeabilized T-47D (human ductal breast epithelial tumor cell line) and negative control line Hs 578T cells stained for Estrogen Receptor alpha (green) using ab3575 (1/2000) in ICC/IF. DyLight 488-conjugated goat anti-rabbit IgG at 1/500.
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab3575 被引用在 15 文献中.
- Huang H et al. Down-regulation of ER-a36 mRNA in serum exosomes of the patients with hepatocellular carcinoma. Clin Transl Med 10:346-352 (2020). PubMed: 32508028
- Kim CS et al. Tamoxifen ameliorates obstructive nephropathy through Src and the PI3K/Akt/mTOR pathway. Biol Cell 111:18-27 (2019). PubMed: 30304547
- Li Q et al. Estradiol promotes the progression of ER+ breast cancer through methylation-mediated RSK4 inactivation. Onco Targets Ther 12:5907-5916 (2019). PubMed: 31413588
- Liu YR et al. ER-depletion lowering the 'hypothalamus-uterus-kidney' axis functions by perturbing the renal ERß/Ptgds signalling pathway. Aging (Albany NY) 11:9500-9529 (2019). PubMed: 31708494
- Niranjan MK & Srivastava R Expression of estrogen receptor alpha in developing brain, ovary and shell gland of Gallus gallus domesticus: Impact of stress and estrogen. Steroids 146:21-33 (2019). PubMed: 30885650
客户评价及客户问答
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