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Signal Transduction Signaling Pathway Nuclear Signaling Nuclear Hormone Receptors Estrogen

Anti-Estrogen Receptor alpha抗体(ab3575)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (13)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab3575)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab3575)
  • Western blot - Anti-Estrogen Receptor alpha antibody (ab3575)
  • Immunocytochemistry - Anti-Estrogen Receptor alpha antibody (ab3575)

Key features and details

  • Rabbit polyclonal to Estrogen Receptor alpha
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Mouse, Rat, Human, Pig, Deer
  • Isotype: IgG

重组抗体的批间可重复性更强

Product image
Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Estrogen Receptor alpha抗体
    参阅全部 Estrogen Receptor alpha 一抗
  • 描述

    兔多克隆抗体to Estrogen Receptor alpha
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC, IHC-P, WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Human, Pig, Deer
    预测可用于: Hamster, Cow, Cat
  • 免疫原

    Synthetic peptide corresponding to Human Estrogen Receptor alpha aa 21-32.
    Sequence:

    NELEPLNRPQLK


    Database link: P03372
    (Peptide available as ab5847)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Estrogen
    • Neuroscience
    • Endocrine system
    • Gonadotrophic axis
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Estrogen
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Estrogen

相关产品

  • Assay kits

    • Estrogen Receptor Transcription Factor Assay Kit (Colorimetric) (ab207203)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunizing Peptide (Blocking)

    • Human Estrogen Receptor alpha peptide (ab5847)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Estrogen Receptor alpha protein (ab82606)

应用

Our Abpromise guarantee covers the use of ab3575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
IHC-P Use a concentration of 1 - 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Estrogen Receptor alpha peptide (ab5847).

靶标

  • 功能

    Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • 序列相似性

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻译后修饰

    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • 细胞定位

    Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Target information above from: UniProt accession P03372 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 407238 Cow
    • Entrez Gene: 101825539 Hamster
    • Entrez Gene: 2099 Human
    • Entrez Gene: 13982 Mouse
    • Entrez Gene: 397435 Pig
    • Entrez Gene: 24890 Rat
    • Omim: 133430 Human
    • SwissProt: P49884 Cow
    • SwissProt: Q9QZJ5 Hamster
    • SwissProt: P03372 Human
    • SwissProt: P19785 Mouse
    • SwissProt: Q29040 Pig
    • SwissProt: P06211 Rat
    • Unigene: 208124 Human
    • Unigene: 463262 Mouse
    • Unigene: 9213 Mouse
    • Unigene: 10595 Rat
    see all
  • 别名

    • 7*/654 isoform antibody
    • 7*/819 2 isoform antibody
    • 7*/822 isoform antibody
    • 8*/901 isoform antibody
    • 8*/941 isoform antibody
    • DKFZp686N23123 antibody
    • ER alpha antibody
    • ER antibody
    • ER-alpha antibody
    • Era antibody
    • ESR antibody
    • ESR1 antibody
    • ESR1_HUMAN antibody
    • ESRA antibody
    • Estradiol receptor antibody
    • Estrogen nuclear receptor alpha antibody
    • Estrogen receptor 1 antibody
    • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
    • Estrogen receptor alpha antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
    • Estrogen receptor alpha delta 4 +49 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
    • Estrogen receptor alpha E1 E2 1 2 antibody
    • Estrogen receptor alpha E1 N2 E2 1 2 antibody
    • Estrogen receptor antibody
    • ESTRR antibody
    • NR3A1 antibody
    • Nuclear receptor subfamily 3 group A member 1 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab3575)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab3575)

    IHC image of Estrogen Receptor alpha staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab3575, 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab3575)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab3575)

    ab3575 at a dilution of 5 µg/ml staining ER alpha in rat prostate tissue sections.

  • Western blot - Anti-Estrogen Receptor alpha antibody (ab3575)
    Western blot - Anti-Estrogen Receptor alpha antibody (ab3575)
    All lanes : Anti-Estrogen Receptor alpha antibody (ab3575) at 1/1000 dilution

    Lane 1 : Recombinant full-length human Estrogen Receptor alpha protein, 250 ng
    Lane 2 : Recombinant full-length human Estrogen Receptor alpha protein, 125 ng
    Lane 3 : Recombinant full-length human Estrogen Receptor alpha protein, 62.5 ng
    Lane 4 : Recombinant full-length human Estrogen Receptor alpha protein, 31.25 ng

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/40000 dilution

    Developed using the ECL technique.

    Predicted band size: 68 kDa



    Blocked with 5% milk in TBST for at least 1 hour at room temperature.

  • Immunocytochemistry - Anti-Estrogen Receptor alpha antibody (ab3575)
    Immunocytochemistry - Anti-Estrogen Receptor alpha antibody (ab3575)

    4% paraformaldehyde fixed 0.1% Triton permeabilized T-47D (human ductal breast epithelial tumor cell line) and negative control line Hs 578T cells stained for Estrogen Receptor alpha (green) using ab3575 (1/2000) in ICC/IF. DyLight 488-conjugated goat anti-rabbit IgG at 1/500.

实验方案

  • Recommended ICC protocol with ab3575 and ab3577

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (13)

    发表研究结果有使用 ab3575?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3575 被引用在 13 文献中.

    • Niranjan MK & Srivastava R Expression of estrogen receptor alpha in developing brain, ovary and shell gland of Gallus gallus domesticus: Impact of stress and estrogen. Steroids 146:21-33 (2019). PubMed: 30885650
    • Glont SE  et al. Identification of ChIP-seq and RIME grade antibodies for Estrogen Receptor alpha. PLoS One 14:e0215340 (2019). PubMed: 30970003
    • Wright KD  et al. The p52 isoform of SHC1 is a key driver of breast cancer initiation. Breast Cancer Res 21:74 (2019). IHC . PubMed: 31202267
    • Kim CS  et al. Tamoxifen ameliorates obstructive nephropathy through Src and the PI3K/Akt/mTOR pathway. Biol Cell 111:18-27 (2019). PubMed: 30304547
    • Li Q  et al. Estradiol promotes the progression of ER+ breast cancer through methylation-mediated RSK4 inactivation. Onco Targets Ther 12:5907-5916 (2019). PubMed: 31413588
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Estrogen Receptor alpha antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Hindlimb muscle)
    Permeabilization
    No
    Specification
    Hindlimb muscle
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 18 2019

    ChIP abreview for Anti-Estrogen Receptor alpha antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (MCF7 breast cancer cell line and tumour samples)
    Specification
    MCF7 breast cancer cell line and tumour samples
    Detection step
    Other
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 30 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Disuccimidyl glutarate (20 min
    Positive control
    Histone acetylation ChIP-seq was used as positive control
    Read More

    Dr. Sankari Nagarajan

    Verified customer

    提交于 Apr 01 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor alpha antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Breast Cancer Metastasis in Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ph6 citrate
    Permeabilization
    No
    Specification
    Breast Cancer Metastasis in Lung
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Dr. Victoria Bridgeman

    Verified customer

    提交于 May 13 2016

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