重组Anti-Estrogen Inducible蛋白pS2抗体[EPR3972] (ab92377)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3972] to Estrogen Inducible Protein pS2
- Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Estrogen Inducible蛋白pS2抗体[EPR3972]
参阅全部 Estrogen Inducible Protein pS2 一抗 -
描述
兔单克隆抗体[EPR3972] to Estrogen Inducible蛋白pS2 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Estrogen Inducible Protein pS2 aa 50-150. The exact sequence is proprietary.
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阳性对照
- WB: Human stomach lysate, MCF-7 cell lysate. IHC: Ductal infiltrating breast carcinoma, Ovarian carcinoma, Human breast carcinoma tissue. IP: MCF7 lysate. Flow cyt: MCF-7 cells. ICC: MCF-7 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
解离常数(KD)
KD = 4.70 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3972 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92377于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 9 kDa.
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IP |
1/10 - 1/100.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 9 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
靶标
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功能
Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine. -
组织特异性
Found in stomach, with highest levels in the upper gastric mucosal cells (at protein level). Detected in goblet cells of the small and large intestine and rectum, small submucosal glands in the esophagus, mucous acini of the sublingual gland, submucosal glands of the trachea, and epithelial cells lining the exocrine pancreatic ducts but not in the remainder of the pancreas (at protein level). Scattered expression is detected in the epithelial cells of the gallbladder and submucosal glands of the vagina, and weak expression is observed in the bronchial goblet cells of the pseudostratified epithelia in the respiratory system (at protein level). Detected in urine (at protein level). Strongly expressed in breast cancer but at low levels in normal mammary tissue. It is regulated by estrogen in MCF-7 cells. Strong expression found in normal gastric mucosa and in the regenerative tissues surrounding ulcerous lesions of gastrointestinal tract, but lower expression found in gastric cancer (at protein level). -
序列相似性
Contains 1 P-type (trefoil) domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 7031 Human
- Omim: 113710 Human
- SwissProt: P04155 Human
- Unigene: 162807 Human
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别名
- BCEI antibody
- Breast cancer estrogen inducible protein antibody
- Breast cancer estrogen inducible sequence antibody
see all
图片
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ab92377 staining Estrogen Inducible Protein pS2 in Formalin-fixed, Paraffin-embedded Human breast carcinoma tissue (A) and Human stomach tissue (B) at 1/100 dilution. Detection: DAB staining.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] (ab92377) at 1/1000 dilution
Lane 1 : Human stomach lysate
Lane 2 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 9 kDa -
ICC/IF image of ab92377 stained MCF7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92377, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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ab92377 showing positive staining in Ovarian carcinoma tissue.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab92377 showing negative staining in Normal breast tissue.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab92377 showing negative staining in Normal kidney tissue.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab92377 showing positive staining in Ductal infiltrating breast carcinoma tissue.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling Estrogen Inducible Protein pS2 with unpurified ab92377 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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Estrogen Inducible Protein pS2 was immunoprecipitated from 0.35 mg MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg with 92377 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: ab92377 IP in MCF7 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab92377 in MCF7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab92377 被引用在 22 文献中.
- Uehara K et al. Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids. Stem Cell Reports 17:820-834 (2022). PubMed: 35245440
- Duijndam B et al. Physiologically Relevant Estrogen Receptor Alpha Pathway Reporters for Single-Cell Imaging-Based Carcinogenic Hazard Assessment of Estrogenic Compounds. Toxicol Sci 181:187-198 (2021). PubMed: 33769548
- Zhou Y et al. Gastrokine 2 Regulates the Antitumor Effect of JAK2/STAT3 Pathway in Gastric Cancer. Evid Based Complement Alternat Med 2021:1343808 (2021). PubMed: 34381519
- Flores AR et al. Immunoexpression of Trefoil Factor 1 in Non-Neoplastic and Neoplastic Canine Gastric Tissues. Animals (Basel) 11:N/A (2021). PubMed: 34679875
- Raghavan S et al. Microenvironment drives cell state, plasticity, and drug response in pancreatic cancer. Cell 184:6119-6137.e26 (2021). PubMed: 34890551