Application
Western blot
Sample
Dog Cell lysate - whole cell (melanoma cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
melanoma cell line
Blocking step
(agent) for 50 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 10% block ace
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
20 minute(s) and 0 second(s)
Additional data
Abcam response
Thank you for your valuable feedback and for participating in our Abtrial testing program. This antibody has not been tested with this WB/Dog before, and it is possible that it may not be suitable for use.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. 令 中野
Verified customer
提交于 Jul 02 2019