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Neuroscience Neurotransmission Intracellular Signaling Kinases

Anti-ERK5 (phospho T218 + Y220)抗体(ab5686)

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Western blot - Anti-ERK5 (phospho T218 + Y220) antibody (ab5686)

    Key features and details

    • Rabbit polyclonal to ERK5 (phospho T218 + Y220)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-ERK5 (phospho T218 + Y220)抗体
      参阅全部 ERK5 一抗
    • 描述

      兔多克隆抗体to ERK5 (phospho T218 + Y220)
    • 宿主

      Rabbit
    • 特异性

      Some cross-reactivity is observed with endogenous ERK1 and 2 (44 and 42 kDa, respectively) due to the high levels of expression and activation of this protein typically observed with most cell types.
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      预测可用于: Mouse
    • 免疫原

      Synthetic phosphopeptide derived from the region of human ERK5 that contains threonine 218 and tyrosine 220.

    • 阳性对照

      • HEK293 cells transiently co-transfected with plasmids expressing ERK5 kinase domain (ERK5kin)and constitutively activated MEK5 (MEK5D-D).
    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated ERK5. The final product is generated by affinity chromatography using an ERK5-derived peptide that is phosphorylated at threonine 218 and tyrosine 220.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Neuroscience
      • Neurotransmission
      • Intracellular Signaling
      • Kinases
      • Neuroscience
      • Neurology process
      • Neural Signal Transduction
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • MAPK Pathway
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • Other Kinases

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human ERK5 protein (ab126913)

    应用

    Our Abpromise guarantee covers the use of ab5686 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB 1/1000. Predicted molecular weight: 88 kDa. Due to the low abundance and low levels of activation of endogenous ERK5, over-expression or immunoprecipitation may be required.

    靶标

    • 功能

      Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways.
    • 组织特异性

      Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver.
    • 序列相似性

      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • 结构域

      The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.
      The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • 翻译后修饰

      Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
    • 细胞定位

      Cytoplasm. Nucleus. Translocates to the nucleus upon activation.
    • Target information above from: UniProt accession Q13164 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 5598 Human
      • Entrez Gene: 23939 Mouse
      • Omim: 602521 Human
      • SwissProt: Q13164 Human
      • SwissProt: Q9WVS8 Mouse
      • Unigene: 150136 Human
      • Unigene: 38172 Mouse
      • 别名

        • Big MAP kinase 1 antibody
        • BMK 1 antibody
        • BMK 1 kinase antibody
        • BMK-1 antibody
        • BMK1 antibody
        • BMK1 Kinase antibody
        • EC 2.7.11.24 antibody
        • ERK 4 antibody
        • ERK 5 antibody
        • ERK-5 antibody
        • ERK4 antibody
        • ERK5 antibody
        • Extracellular signal regulated kinase 5 antibody
        • Extracellular signal-regulated kinase 5 antibody
        • MAP kinase 7 antibody
        • MAPK 7 antibody
        • MAPK7 antibody
        • Mitogen activated protein kinase 7 antibody
        • Mitogen-activated protein kinase 7 antibody
        • MK07_HUMAN antibody
        • OTTHUMP00000065906 antibody
        • OTTHUMP00000065907 antibody
        • PRKM 7 antibody
        • PRKM7 antibody
        • PROTEIN KINASE, MITOGEN-ACTIVATED, 7 antibody
        see all

      图片

      • Western blot - Anti-ERK5 (phospho T218 + Y220) antibody (ab5686)
        Western blot - Anti-ERK5 (phospho T218 + Y220) antibody (ab5686)
        Peptide Competition:
        Extracts prepared from HEK293 cells transiently transfected with plasmids expressing ERK5 kinase domain (ERK5kin) and constitutively activated MEK5D-D were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA TBST buffer overnight at 4oC, then were incubated with the ab5686 antibody for two hours at room temperature in a 3% BSATBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), a generic phosphotyrosine-containing peptide (4), the phosphopeptide derived from the corresponding region of ERK1&2 (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG alkaline phosphatase conjugate and bands were detected using the Tropix WesternStarTM detection method. The data show that while there is some cross-reactivity with ERK1&

      实验方案

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet
    • 文献 (2)

      发表研究结果有使用 ab5686?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab5686 被引用在 2 文献中.

      • Song B  et al. LncRNA ENST00000539653 acts as an oncogenic factor via MAPK signalling in papillary thyroid cancer. BMC Cancer 19:297 (2019). PubMed: 30940124
      • Govarts C  et al. Analysis of antibody reactivity in paired cerebrospinal fluid and serum of a relapsing remitting multiple sclerosis patient. Autoimmunity 42:699-704 (2009). IHC-Fr ; Human . PubMed: 19835487

      客户评价及客户问答

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      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-ERK5 (phospho T218 + Y220) antibody

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Dog Cell lysate - whole cell (melanoma cell line)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      10 µg
      Specification
      melanoma cell line
      Blocking step
      (agent) for 50 minute(s) · Concentration: 100% · Temperature: 25°C
      Read More

      Dr. 令 中野

      Verified customer

      提交于 Jul 02 2019

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ERK5 (phospho T218 + Y220) antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Heart)
      Antigen retrieval step
      None
      Permeabilization
      Yes - 0.2% PBS-Triton X100
      Specification
      Heart
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Ricardo Santamaría

      Verified customer

      提交于 Apr 11 2017

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