Anti-ERK5 (phospho T218 + Y220)抗体(ab5686)
Key features and details
- Rabbit polyclonal to ERK5 (phospho T218 + Y220)
- Suitable for: WB, IHC-Fr
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ERK5 (phospho T218 + Y220)抗体
参阅全部 ERK5 一抗 -
描述
兔多克隆抗体to ERK5 (phospho T218 + Y220) -
宿主
Rabbit -
特异性
Some cross-reactivity is observed with endogenous ERK1 and 2 (44 and 42 kDa, respectively) due to the high levels of expression and activation of this protein typically observed with most cell types. -
经测试应用
适用于: WB, IHC-Frmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic phosphopeptide derived from the region of human ERK5 that contains threonine 218 and tyrosine 220.
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阳性对照
- HEK293 cells transiently co-transfected with plasmids expressing ERK5 kinase domain (ERK5kin)and constitutively activated MEK5 (MEK5D-D).
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated ERK5. The final product is generated by affinity chromatography using an ERK5-derived peptide that is phosphorylated at threonine 218 and tyrosine 220. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab5686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 88 kDa. Due to the low abundance and low levels of activation of endogenous ERK5, over-expression or immunoprecipitation may be required. | |
| IHC-Fr | Use at an assay dependent concentration. |
靶标
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功能
Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. -
组织特异性
Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域
The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻译后修饰
Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent. -
细胞定位
Cytoplasm. Nucleus. Translocates to the nucleus upon activation. - Information by UniProt
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数据库链接
- Entrez Gene: 5598 Human
- Entrez Gene: 23939 Mouse
- Omim: 602521 Human
- SwissProt: Q13164 Human
- SwissProt: Q9WVS8 Mouse
- Unigene: 150136 Human
- Unigene: 38172 Mouse
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别名
- Big MAP kinase 1 antibody
- BMK 1 antibody
- BMK 1 kinase antibody
see all
图片
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Western blot - Anti-ERK5 (phospho T218 + Y220) antibody (ab5686)Peptide Competition:
Extracts prepared from HEK293 cells transiently transfected with plasmids expressing ERK5 kinase domain (ERK5kin) and constitutively activated MEK5D-D were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA TBST buffer overnight at 4oC, then were incubated with the ab5686 antibody for two hours at room temperature in a 3% BSATBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), a generic phosphotyrosine-containing peptide (4), the phosphopeptide derived from the corresponding region of ERK1&2 (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG alkaline phosphatase conjugate and bands were detected using the Tropix WesternStarTM detection method. The data show that while there is some cross-reactivity with ERK1&
数据表及文件
文献 (2)
ab5686 被引用在 2 文献中.
- Song B et al. LncRNA ENST00000539653 acts as an oncogenic factor via MAPK signalling in papillary thyroid cancer. BMC Cancer 19:297 (2019). PubMed: 30940124
- Govarts C et al. Analysis of antibody reactivity in paired cerebrospinal fluid and serum of a relapsing remitting multiple sclerosis patient. Autoimmunity 42:699-704 (2009). IHC-Fr ; Human . PubMed: 19835487
