Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187)抗体(ab128159)
Key features and details
- Rabbit polyclonal to ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187)
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187)抗体 -
描述
兔多克隆抗体to ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Goat, Chicken, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
免疫原
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阳性对照
- HeLa whole cell lysate, mock treated or treated with TPA
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128159于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 41, 43 kDa.
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IP |
Use at an assay dependent concentration.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 41, 43 kDa. |
IP
Use at an assay dependent concentration. |
靶标
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细胞定位
ERK2: Nucleus. -
数据库链接
- Entrez Gene: 373953 Chicken
- Entrez Gene: 327672 Cow
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Entrez Gene: 26413 Mouse
- Entrez Gene: 26417 Mouse
- Entrez Gene: 100153927 Pig
- Entrez Gene: 116590 Rat
see all
图片
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All lanes : Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody (ab128159) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate, mock treated
Lane 2 : HeLa whole cell lysate, TPA treated
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 41, 43 kDa
Exposure time: 30 seconds -
ab128159 at 1 µg/ml staining ERK1 + ERK2 (phospho T202 + Y204 + T185 + Y187) in HeLa whole cell lysate immunoprecipitated using a Rabbit anti-ERK1 antibody (1 mg/IP; 20% of IP loaded/lane).
Lane 1: mock treated.
Lanes 2 and 3: TPA treated.
Lane 3: normal goat IgG.
Predicted band size: 41 kDa.
Exposure time: 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab128159 尚未被引用在任何文献中。