产品名称Anti-ERK1 + ERK2抗体[EPR17526] (Alexa Fluor® 405)
参阅全部 ERK1 + ERK2 一抗
描述兔单克隆抗体[EPR17526] to ERK1 + ERK2 (Alexa Fluor® 405)
偶联物Alexa Fluor® 405. Ex: 402nm, Em: 421nm
经测试应用适用于: Flow Cytmore details
预测可用于: Mouse, Rat
Recombinant fragment within Human ERK1 + ERK2 aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: P28482
- Flow Cyt: A431 cells.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol, PBS
Concentration information loading...
纯度Protein A purified
- Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
- Anti-ERK1 + ERK2 antibody [EPR17526] (Alexa Fluor® 488) (ab208564)
- Anti-ERK1 + ERK2 antibody [EPR17526] (Alexa Fluor® 647) (ab208881)
- Anti-ERK1 + ERK2 antibody [EPR17526] (HRP) (ab209321)
- Anti-ERK1 + ERK2 antibody [EPR17526] (Alexa Fluor® 594) (ab210460)
- Anti-ERK1 + ERK2 antibody [EPR17526] (Alexa Fluor® 555) (ab210659)
- Anti-ERK1 + ERK2 antibody [EPR17526] (Phycoerythrin) (ab212153)
- Anti-ERK1 + ERK2 antibody [EPR17526] - BSA and Azide free (ab218017)
Our Abpromise guarantee covers the use of ab210044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
功能Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
结构域The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
翻译后修饰Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
- Information by UniProt
- ERK 1 antibody
- ERK 2 antibody
- ERK-2 antibody
Overlay histogram showing A431 cells stained with ab210044 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min, blocked with Image-iT® FX Signal Enhancer for 30 min at 22ºC and then blocked with 1x PBS / 10% normal goat serum for 1 hr at 22ºC. Cells were then incubated with the antibody (ab210044, 1/500 dilution) for 30 min at 22ºC.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 405 (ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW violet laser (405nm) and 450/50 bandpass filter.
ab210044 has not yet been referenced specifically in any publications.