Anti-ERG抗体(ab96697)
Key features and details
- Rabbit polyclonal to ERG
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-ERG抗体
参阅全部 ERG 一抗 -
描述
兔多克隆抗体to ERG -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human ERG aa 445-457.
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阳性对照
- WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates ICC/IF: Human pancreatic cancer cell line
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96697于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 55 kDa.
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ICC/IF |
1/50.
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说明 |
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WB
1/1000. Predicted molecular weight: 55 kDa. |
ICC/IF
1/50. |
靶标
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功能
Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure. -
疾病相关
Defects in ERG are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving ERG is found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1.
Note=Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS. Translocation t(X;21)(q25-26;q22) with ELF4. -
序列相似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
细胞定位
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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数据库链接
- Entrez Gene: 2078 Human
- Omim: 165080 Human
- SwissProt: P11308 Human
- Unigene: 473819 Human
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别名
- Avian erythroblastosis virus E-26 (v-ets) oncogene related antibody
- D030036I24Rik antibody
- Erg 3 antibody
see all
图片
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Anti-ERG antibody (ab96697) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 55 kDa
10% SDS PAGE -
Immunofluorescence analysis of ERG in paraformaldehyde fixed Human pancreatic cancer cell line, using ab96697 at a 1/50 dilution.
Lower image: merged with DNA probe. -
Immunofluorescence analysis of ERG in paraformaldehyde fixed Human pancreatic cancer cell line, using ab96697 at a 1/50 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab96697 被引用在 1 文献中.
- Polson ES et al. Monoallelic expression of TMPRSS2/ERG in prostate cancer stem cells. Nat Commun 4:1623 (2013). Human . PubMed: 23535644