概述

  • 产品名称
    Anti-ErbB 2抗体[EP1045Y]
    参阅全部 ErbB 2 一抗
  • 描述
    兔单克隆抗体[EP1045Y] to ErbB 2
  • 宿主
    Rabbit
  • 特异性
    ab134182 detects ErbB 2 phosphorylated at Tyr1248 as well as unphosphorylated ErbB 2.
  • 经测试应用
    适用于: WB, IP, ICC/IF, IHC-Pmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Human
  • 免疫原

    within Human ErbB 2 aa 1200 to the C-terminus (C terminal). The exact sequence is proprietary.

  • 阳性对照
    • WB: HeLa, SKBR-3 cell lysate; IHC-P: Human breast carcinoma tissue; ICC/IF: SKBR cells; IP: HeLa.
  • 常规说明

     

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab134182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 137 kDa.
IP 1/30.

For unpurifed use at 1/50.

ICC/IF 1/250 - 1/500.
IHC-P 1/1600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/100 -1/250. 

  • 应用说明
    Is unsuitable for Flow Cyt.
  • 靶标

    • 功能
      Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
      In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
    • 组织特异性
      Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
    • 疾病相关
      Hereditary diffuse gastric cancer
      Glioma
      Ovarian cancer
      Lung cancer
      Gastric cancer
      Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2.
    • 序列相似性
      Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰
      Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172).
    • 细胞定位
      Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
      • C erb B2/neu protein antibody
      • CD340 antibody
      • CD340 antigen antibody
      • Cerb B2/neu protein antibody
      • CerbB2 antibody
      • Erb b2 receptor tyrosine kinase 2 antibody
      • ERBB2 antibody
      • ERBB2_HUMAN antibody
      • HER 2 antibody
      • HER 2/NEU antibody
      • HER2 antibody
      • Herstatin antibody
      • Human epidermal growth factor receptor 2 antibody
      • Metastatic lymph node gene 19 protein antibody
      • MLN 19 antibody
      • MLN19 antibody
      • NEU antibody
      • NEU proto oncogene antibody
      • Neuro/glioblastoma derived oncogene homolog antibody
      • Neuroblastoma/glioblastoma derived oncogene homolog antibody
      • NGL antibody
      • p185erbB2 antibody
      • Proto-oncogene c-ErbB-2 antibody
      • Proto-oncogene Neu antibody
      • Receptor tyrosine-protein kinase erbB-2 antibody
      • TKR1 antibody
      • Tyrosine kinase type cell surface receptor HER2 antibody
      • Tyrosine kinase-type cell surface receptor HER2 antibody
      • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody
      • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 antibody
      • V erb b2 avian erythroblastic leukemia viral oncoprotein 2 antibody
      • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
      • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
      • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
      see all

    图片

    • Anti-ErbB 2 antibody [EP1045Y] (ab134182) at 1/1000 dilution + 4T1(Mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 137 kDa
      Observed band size: 180 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling ErbB 2 with Purified ab134182 at 1:1600 dilution (0.68 µg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
    • ab134182 (purified) at 1:30 dilution (2µg) immunoprecipitating ErbB 2 in HeLa whole cell lysate.
      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab134182 & HeLa whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab134182 in HeLa whole cell lysate
      For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.
    • Anti-ErbB 2 antibody [EP1045Y] (ab134182) at 1/10000 dilution (purified) + SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 137 kDa



      Blocking and diluting buffer : 5% NFDM/TBST
    • Anti-ErbB 2 antibody [EP1045Y] (ab134182) at 1/1000 dilution (purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 137 kDa



      Blocking and diluting buffer : 5% NFDM/TBST
    • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ErbB 2 with ab134182 at 1/100 dilution.
    • Immunofluorescent analysis of SKBR cells labelling ErbB 2 with ab134182 at 1/250 dilution.
    • Immunocytochemistry/Immunofluorescence analysis of SK-BR-3 (human mammary gland adenocarcinoma) labelling ErbB 2 with purified ab134182 at 1/125. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

      Control: PBS only

    • Anti-ErbB 2 antibody [EP1045Y] (ab134182) at 1/10000 dilution + SKBR-3 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 137 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    文献

    This product has been referenced in:
    • Guo C  et al. microRNA-10b expression and its correlation with molecular subtypes of early invasive ductal carcinoma. Exp Ther Med 15:2851-2859 (2018). Read more (PubMed: 29599829) »
    • Yu W  et al. Prognostic value of EGFR family expression in lymph node-negative esophageal squamous cell carcinoma patients. Pathol Res Pract 214:1017-1023 (2018). IHC-P ; Human . Read more (PubMed: 29729836) »
    See all 17 Publications for this product

    客户评价及客户问答

    1-2 of 2 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    No
    Fixative
    Paraformaldehyde

    Dr. Kirk Mcmanus

    Verified customer

    提交于 Mar 27 2014

    Answer

    Thank you for contacting us. If you are conjugating the antibody to a protein, then you probably want the antibody formulation to be free of other proteins such as bovine serum albumin (BSA). The concentration of the antibody may need to be at least 1mg/ml, but your conjugation protocol will tell you if this is necessary. The concentration of ab134182 is lot dependent and may be less than 1mg/ml. You can contact us for specific lot concentrations. I recommend ab36728. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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