Anti-Eph receptor A4/SEK抗体(ab5396)
Key features and details
- Rabbit polyclonal to Eph receptor A4/SEK
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Eph receptor A4/SEK抗体
参阅全部 Eph receptor A4/SEK 一抗 -
描述
兔多克隆抗体to Eph receptor A4/SEK -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Eph receptor A4/SEK aa 875-904 (C terminal) conjugated to keyhole limpet haemocyanin.
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常规说明
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
This product was previously labelled as Eph receptor A4
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS. -
Primary antibody说明
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab5396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 118 kDa). We recommend using CHAPS lysis buffer rather than RIPA, Invitrogen NuPage gel system, blotting on PVDF membrane, blocking 30' in 10% Fetal Bovine Serum, 3% BSA, 0.3% Tween 20 in 1X PBS - please see Abreview for further information (published on 24th July, 2006). |
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| IHC-P | 1/25 - 1/100. |
靶标
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功能
Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in a signal transduction process involved in hindbrain pattern formation. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
结构域
The protein kinase domain mediates interaction with NGEF/ephexin-1. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2043 Human
- Entrez Gene: 13838 Mouse
- Entrez Gene: 316539 Rat
- Omim: 602188 Human
- SwissProt: P54764 Human
- SwissProt: Q03137 Mouse
- Unigene: 371218 Human
- Unigene: 400747 Mouse
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别名
- Cek 8 antibody
- CEK8 antibody
- EK8 antibody
see all
图片
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Western blot - Anti-Eph receptor A4/SEK antibody (ab5396)All lanes : Anti-Eph receptor A4/SEK antibody (ab5396) at 1/1000 dilution
Lane 1 : HeLa Cell Lysate
Lane 2 : NCI-H460 Cell Lysate
Lane 3 : Mouse NIH3T3 Cell Lysate
Lane 4 : Human ovary tissue Lysate
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG H+L HRP conjugated at 1/10000 dilution
Predicted band size: 118 kDaIncubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot - Anti-Eph receptor A4/SEK antibody (ab5396)Anti-Eph receptor A4/SEK antibody (ab5396) at 1/1000 dilution + NCI-H460 whole cell lysate at 35 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution
Predicted band size: 118 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor A4/SEK antibody (ab5396)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling Eph receptor A4/SEK with ab5396 at dilution 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C; heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.
数据表及文件
文献 (4)
ab5396 被引用在 4 文献中.
- Feng L et al. EphA4 may contribute to microvessel remodeling in the hippocampal CA1 and CA3 areas in a mouse model of temporal lobe epilepsy. Mol Med Rep 15:37-46 (2017). PubMed: 27959424
- Todd KL et al. EphA4 Regulates Neuroblast and Astrocyte Organization in a Neurogenic Niche. J Neurosci 37:3331-3341 (2017). ICC/IF ; Mouse . PubMed: 28258169
- Fan R et al. Critical role of EphA4 in early brain injury after subarachnoid hemorrhage in rat. Exp Neurol 296:41-48 (2017). PubMed: 28698029
- Wu LY et al. Connexin 43 stabilizes astrocytes in a stroke-like milieu to facilitate neuronal recovery. Acta Pharmacol Sin 36:928-38 (2015). PubMed: 26095039
