参阅全部 EPF 一抗
描述兔单克隆抗体[EPR4475] to EPF
经测试应用适用于: WB, IP, Flow Cytmore details
不适用于: ICC or IHC-P
种属反应性与反应: Rat, Human
Synthetic peptide within Human EPF aa 50-150. The exact sequence is proprietary.
- HeLa, HepG2, Raji, and Jurkat cell lysates. Permeabilized Jurkat cells.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
纯度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab108611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
功能Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.
序列相似性Belongs to the GroES chaperonin family.
- Information by UniProt
- 10 kDa chaperonin antibody
- 10 kDa heat shock protein mitochondrial antibody
- 10 kDa heat shock protein, mitochondrial antibody
All lanes : Anti-EPF antibody [EPR4475] (ab108611) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Flow cytometric analysis of permeabilized Jurkat cells using ab108611 at 1/10 (red) or a Rabbit IgG (negative) (green).
ab108611 has not yet been referenced specifically in any publications.