概述

  • 产品名称
    Anti-EPB41L5抗体
    参阅全部 EPB41L5 一抗
  • 描述
    山羊多克隆抗体to EPB41L5
  • 宿主
    Goat
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide: C-

    ENLPQSPGTDQHD

    , corresponding to internal sequence amino acids 431-443 of Human EPB41L5 (NP_065960.2).

性能

应用

Our Abpromise guarantee covers the use of ab78607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 81.9 kDa).
ELISA Use at an assay dependent concentration. Peptide ELISA antibody detection limit dilution 1:8000.

靶标

  • 功能
    May contribute to the correct positioning of tight junctions during the establishment of polarity in epithelial cells.
  • 序列相似性
    Contains 1 FERM domain.
  • 结构域
    The FERM domain binds to CRB1.
  • 细胞定位
    Cytoplasm. Cell junction > adherens junction. Associated with the plasma membrane. Colocalizes with CTNNB1, an adherens junction marker.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Band 4.1-like protein 5 antibody
    • BE37 antibody
    • E41L5_HUMAN antibody
    • Epb41l5 antibody
    • erythrocyte membrane protein band 4.1 like 5 antibody
    • KIAA1548 antibody
    see all

图片

  • Anti-EPB41L5 antibody (ab78607) at 1 µg/ml + Human Brain (Hippocampus) Lysate in RIPA buffer at 35 µg

    Predicted band size: 81.9 kDa



    Primary incubation was 1 hour.

实验方案

文献

ab78607 has not yet been referenced specifically in any publications.

客户评价及客户问答

1-2 of 2 Q&A

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab138986. The estimated delivery date is xxx.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Answer

Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody.

The details your customer has kindly provided will enable us to investigate this case for your customer and this is also helpful in our records for monitoring of quality.

Although the other antibody has worked well using this procedure, individual antibodies will often require optimization. I would like to offer some suggestions to help optimise the results from ab78607.

This antibody has not been tested on recombinant and tagged protein.

1. There are inherent difficulties with antibody detection of recombinant tagged proteins that I can recommend considering. This may be a biological artifact rather than a problem with the antibody. Also, if the recombinant EPB41L5 is tagged, it is possible that the tag is preventing access to the antibody. Could you confirm at which region of the protein the tag has been placed?

2. Could you confirm the species from which the recombinant EPB41L5 gene was taken?

3. I can recommend that due to the points described above, it is important to use an endogenous positive control sample. For example, this antibody has been used successfully on human brain lysate.

4. I can also recommend to run a no primary control to see if the band might be due to the secondary antibody.

We are happy to offer this technical support. In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I hope this information is helpful, thank you and your customer for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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