Anti-Endothelin 1抗体[TR.ET.48.5] (ab2786)
Key features and details
- Mouse monoclonal [TR.ET.48.5] to Endothelin 1
- Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Sheep, Rabbit, Human, Pig
- Isotype: IgG1
概述
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产品名称
Anti-Endothelin 1抗体[TR.ET.48.5]
参阅全部 Endothelin 1 一抗 -
描述
小鼠单克隆抗体[TR.ET.48.5] to Endothelin 1 -
宿主
Mouse -
特异性
Immunohistochemical staining of ET-1 in human corpus cavernosum tissue with this antibody results in staining of endothelial cells. Radioimmune assays can be used to concentrate ET-1 in solution (e.g. serum/plasma, milk, urine). -
经测试应用
适用于: Flow Cyt, ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Sheep, Rabbit, Human, Pig -
免疫原
Full length protein corresponding to Human Endothelin 1 conjugated to Keyhole Limpet Haemocyanin (KLH).
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表位
Studies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16. -
阳性对照
- human bowel
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
TR.ET.48.5 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab2786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt | Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF | 1/200 - 1/1000. | |
IHC-P | 1/250. | |
WB | Use at an assay dependent concentration. Predicted molecular weight: 24 kDa. |
靶标
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功能
Endothelins are endothelium-derived vasoconstrictor peptides. -
组织特异性
Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. -
序列相似性
Belongs to the endothelin/sarafotoxin family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 1906 Human
- Entrez Gene: 13614 Mouse
- Entrez Gene: 396915 Pig
- Entrez Gene: 100009270 Rabbit
- Entrez Gene: 24323 Rat
- Entrez Gene: 443498 Sheep
- Omim: 131240 Human
- SwissProt: P05305 Human
see all -
别名
- ARCND3 antibody
- Big endothelin-1 antibody
- EDN1 antibody
see all
图片
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ab2786 labelling Endothelin 1 (green) in the secretion of HeLa cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.
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Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786) at 1/500 dilution + PC12 cell lysate at 25 µg
Predicted band size: 24 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
Flow cytometry analysis of Endothelin 1 showing positive staining in the cytoplasm of 293T cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ab2786 (0.5 ug/test) for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.
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ab2786 labelling Endothelin 1 (green) in the secretion of PC12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.
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Flow cytometry analysis of Endothelin 1 showing positive staining in the cytoplasm of HepG2 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ab2786 (0.5 ug/test) for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.
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ab2786 labelling Endothelin 1 (green) in the secretion of HUVEC cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.
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Flow cytometry analysis of Endothelin 1 showing positive staining in the cytoplasm of 3T3 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ab2786 (0.5 ug/test) for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)Figure 1 and Figure 2 show immunolocalization of ET-1 in human bowel using ab2786.
实验方案
文献 (39)
ab2786 被引用在 39 文献中.
- Qin R et al. Mst1 deletion reduces hyperglycemia-mediated vascular dysfunction via attenuating mitochondrial fission and modulating the JNK signaling pathway. J Cell Physiol 235:294-303 (2020). PubMed: 31206688
- Zhai R et al. Exendin-4, a GLP-1 receptor agonist regulates retinal capillary tone and restores microvascular patency after ischaemia-reperfusion injury. Br J Pharmacol 177:3389-3402 (2020). PubMed: 32232832
- Zhang J et al. MicroRNA-483 amelioration of experimental pulmonary hypertension. EMBO Mol Med 12:e11303 (2020). PubMed: 32324970
- Liu SX et al. Exercise Reduces Insulin Resistance in Type 2 Diabetes Mellitus via Mediating the lncRNA MALAT1/MicroRNA-382-3p/Resistin Axis. Mol Ther Nucleic Acids 18:34-44 (2019). PubMed: 31479923
- Wang H et al. Modulating effects of preconditioning exercise in the expression of ET-1 and BNP via HIF-1a in ischemically injured brain. Metab Brain Dis 34:1299-1311 (2019). PubMed: 31222402