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Signal Transduction Growth Factors/Hormones Hormones

Anti-Endothelin 1抗体[TR.ET.48.5] (ab2786)

  • Datasheet
  • SDS
Submit a review Q&A (8)References (39)

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Immunocytochemistry/ Immunofluorescence - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)
  • Western blot - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)
  • Flow Cytometry - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)
  • Immunocytochemistry/ Immunofluorescence - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)
  • Flow Cytometry - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)
  • Immunocytochemistry/ Immunofluorescence - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)
  • Flow Cytometry - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)

Key features and details

  • Mouse monoclonal [TR.ET.48.5] to Endothelin 1
  • Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Sheep, Rabbit, Human, Pig
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Endothelin 1抗体[TR.ET.48.5]
    参阅全部 Endothelin 1 一抗
  • 描述

    小鼠单克隆抗体[TR.ET.48.5] to Endothelin 1
  • 宿主

    Mouse
  • 特异性

    Immunohistochemical staining of ET-1 in human corpus cavernosum tissue with this antibody results in staining of endothelial cells. Radioimmune assays can be used to concentrate ET-1 in solution (e.g. serum/plasma, milk, urine).
  • 经测试应用

    适用于: Flow Cyt, ICC/IF, IHC-P, WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Sheep, Rabbit, Human, Pig
  • 免疫原

    Full length protein corresponding to Human Endothelin 1 conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 表位

    Studies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16.
  • 阳性对照

    • human bowel
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    TR.ET.48.5
  • 同种型

    IgG1
  • 研究领域

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Cardiovascular
    • Vasculature
    • Vasoconstriction
    • Other
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Endothelin 1 protein (ab158332)

应用

Our Abpromise guarantee covers the use of ab2786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/200 - 1/1000.
IHC-P 1/250.
WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.

靶标

  • 功能

    Endothelins are endothelium-derived vasoconstrictor peptides.
  • 组织特异性

    Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.
  • 序列相似性

    Belongs to the endothelin/sarafotoxin family.
  • 细胞定位

    Secreted.
  • Target information above from: UniProt accession P05305 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1906 Human
    • Entrez Gene: 13614 Mouse
    • Entrez Gene: 396915 Pig
    • Entrez Gene: 100009270 Rabbit
    • Entrez Gene: 24323 Rat
    • Entrez Gene: 443498 Sheep
    • Omim: 131240 Human
    • SwissProt: P05305 Human
    • SwissProt: P22387 Mouse
    • SwissProt: P09558 Pig
    • SwissProt: P29560 Rabbit
    • SwissProt: P22388 Rat
    • SwissProt: Q9BG76 Sheep
    • Unigene: 511899 Human
    • Unigene: 713645 Human
    • Unigene: 10918 Rat
    see all
  • 别名

    • ARCND3 antibody
    • Big endothelin-1 antibody
    • EDN1 antibody
    • EDN1_HUMAN antibody
    • ET-1 antibody
    • ET1 antibody
    • HDLCQ7 antibody
    • PPET1 antibody
    • Preproendothelin 1 antibody
    • Preproendothelin-1 antibody
    • QME antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)
    Immunocytochemistry/ Immunofluorescence - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)

    ab2786 labelling Endothelin 1 (green) in the secretion of HeLa cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • Western blot - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)
    Western blot - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)
    Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786) at 1/500 dilution + PC12 cell lysate at 25 µg

    Predicted band size: 24 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)
    Flow Cytometry - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)

    Flow cytometry analysis of Endothelin 1 showing positive staining in the cytoplasm of 293T cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ab2786 (0.5 ug/test) for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)
    Immunocytochemistry/ Immunofluorescence - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)

    ab2786 labelling Endothelin 1 (green) in the secretion of PC12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • Flow Cytometry - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)
    Flow Cytometry - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)

    Flow cytometry analysis of Endothelin 1 showing positive staining in the cytoplasm of HepG2 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ab2786 (0.5 ug/test) for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)
    Immunocytochemistry/ Immunofluorescence - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)

    ab2786 labelling Endothelin 1 (green) in the secretion of HUVEC cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L)  was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • Flow Cytometry - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)
    Flow Cytometry - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)

    Flow cytometry analysis of Endothelin 1 showing positive staining in the cytoplasm of 3T3 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ab2786 (0.5 ug/test) for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)
    Figure 1 and Figure 2 show immunolocalization of ET-1 in human bowel using ab2786.

实验方案

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (39)

    发表研究结果有使用 ab2786?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2786 被引用在 39 文献中.

    • Qin R  et al. Mst1 deletion reduces hyperglycemia-mediated vascular dysfunction via attenuating mitochondrial fission and modulating the JNK signaling pathway. J Cell Physiol 235:294-303 (2020). PubMed: 31206688
    • Zhai R  et al. Exendin-4, a GLP-1 receptor agonist regulates retinal capillary tone and restores microvascular patency after ischaemia-reperfusion injury. Br J Pharmacol 177:3389-3402 (2020). PubMed: 32232832
    • Zhang J  et al. MicroRNA-483 amelioration of experimental pulmonary hypertension. EMBO Mol Med 12:e11303 (2020). PubMed: 32324970
    • Liu SX  et al. Exercise Reduces Insulin Resistance in Type 2 Diabetes Mellitus via Mediating the lncRNA MALAT1/MicroRNA-382-3p/Resistin Axis. Mol Ther Nucleic Acids 18:34-44 (2019). PubMed: 31479923
    • Wang H  et al. Modulating effects of preconditioning exercise in the expression of ET-1 and BNP via HIF-1a in ischemically injured brain. Metab Brain Dis 34:1299-1311 (2019). PubMed: 31222402
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-8 of 8 Abreviews or Q&A

    Question

    Dear Sir,Madam, Many thanks for your help.I have another question.For anti-Endothelin 1 antibody(ab2786) would you please inform me what is cellular localization exactly? And if it is possible send me some photos for positive staining. With best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jan 12 2012

    Answer

    Thank you for your reply and further enqjuiry. I am sorry to confirm that the IHC image provided on the datasheet is the only one available at present. There are also 6 publications cited on the datasheet which have used this antibody. Ican suggest to review the data within these for further reassurance: Mayyas F et al. Dietary 3 fatty acids modulate the substrate for post-operative atrial fibrillation in a canine cardiac surgery model. Cardiovasc Res 89:852-61 (2011). Dog. PubMed: 21123218 Chen CL et al. Increased endothelin 1 expression in adult-onset minimal change nephropathy with acute renal failure. Am J Kidney Dis 45:818-25 (2005). PubMed: 15861346 Nelson JB et al. Endothelin-1 production and decreased endothelin B receptor expression in advanced prostate cancer. Cancer Res 56:663-8 (1996). PubMed: 8630991 Traish AM et al. Monoclonal antibodies to human endothelin-1: characterization and utilization in radioimmunoassay and immunocytochemistry. Hybridoma 11:147-63 (1992). PubMed: 1607212 Rubanyi GM & Botelho LH Endothelins. FASEB J 5:2713-20 (1991). PubMed: 1916094 Saenz de Tejada I et al. Endothelin: localization, synthesis, activity, and receptor types in human penile corpus cavernosum. Am J Physiol 261:H1078-85 (1991). PubMed: 1656784 The cellular localisation of Endothelin 1 is 'secreted. Therefore, in tissue, it is likely to be localised between the cells. There may be some cytoplasmic staining of Endothelin that has not yet been secreted from the cells. I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    回复于 Jan 12 2012

    Question

    Dear Sir,Madam, Many thanks for your reply. I am going to do IHC on Thyroid cancer (parrafin block tissues).My antibodies are Anti-Endothelin -1 (ab2786) and Anti-Endothelin A Receptor (ab76259).   I would be very grateful if you suggest me both positive and negative control tissues.   With best regards,    

    Read More

    Abcam community

    Verified customer

    Asked on Dec 20 2011

    Answer

    Thank you for your message and for kindly providing the requested details. I am pleased to provide the following information: Endothelin -1 antibody ab2786 According to the image on the datasheet, this antibody has been successfully used in  human bowel samples, and I can suggest this would be a suitable positive control. According to the SwissProt/Uniprot protein database site, it is also expressed in the lung and  placental stem villi vessels and these would also be suitable positive controls.  As endothelin 1 is found in the smooth muscle tissue of blood vessels, it will be found in most tissue types. therefore, it would be difficult to suggest a negative control. I can recommend a literature search to obtain further information. Endothelin A Receptor antibody ab76259 According to the image on the datasheet, this antibody has been successfully used in  human cerebellum samples, and I can suggest this would be a suitable positive control. Its tissue specificity, from the SwissProt/Uniprot protein database link is as follows: Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels.  Therefore, these tissues would also be suitable positive controls. Again, Endothelin A receptor it is quite widely expressed, and so suggesting a suitable negative control is difficult.   I hope this information is helpful to you. Should you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    回复于 Dec 20 2011

    Question

    Customer would like to purchase 2 Abcam antibodies, ab2786 and ab14672, to use in human parrafin tissues. However, her samples are from necropsy and she is concerned about the fixation time and the antibody binding capacity. Is there anything we could do to help her in this issue? Or the antibodies will not react at all with tissues from necropsy? I look forward to hearing from you. Best Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 19 2011

    Answer

    Thank you for your enquiry. With necrotic tissue, it is very likely that the proteins are already being broken down and degraded by the enzymes that are already present. I am sorry it would be very difficult to say and guarantee how much Endothelin 1 or PDE5A would still be in the sample for detection by the antibody. If the proteins are released from the cell during necrosis, the localization of the protein may also be affected.  If the tissue is already necrotic before fixation, then there are no fixation methods that would specifically help in this case. I can suggest to try the usual fixation methods. I can suggest it would be important to consider including positive control tissue. For example: PDE5A antibody (ab14672) has been used successfully in rat human heart tissue. We do have the following slides available: ab4332  Heart (human): aortic valve normal tissue slides Applications: IHC-P, ISH   Endothelin 1 antibody (ab2786) has been used successfully in human bowel. We do have the following slides available: ab4327 Colon (human) normal tissue slides Applications: IHC-P, ISH    I hope this information will be helpful to you.  If you have any further questions, please do not hesitate to contact me.    

    Read More

    Abcam Scientific Support

    回复于 Oct 19 2011

    Question

    does this antibody recognize just mature ET? Can it recognize preproET or big ET? Can it recognize ET in cell and secreted ET?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 21 2006

    Answer

    Thank you for your enquiry. I'm afraid we have no data regarding the detection of PreproET or Big ET using this antibody at this time. Both ET-1 and Big ET are contained within PreproET. Therefore I would expect that this antibody would detect PreproET as well, however I have no data to support this hypothesis and therefore I cannot guarantee it will detect prepro and big ET. The antibody has been used in immunohistochemical staining of ET-1 in human corpus cavernosum tissue with this antibody resulting in staining of endothelial cells and it also has successfully been used in radioimmune assays to concentrate ET-1 in solution (e.g. serum/plasma, milk, urine). Please do not hesitate to contact me if I can be of further assistance,

    Read More

    Abcam Scientific Support

    回复于 Nov 23 2006

    Question

    What is the concentration of this antibody, so I can run a negative control for it?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 03 2006

    Answer

    Thank you for your enquiry. It is not possible to determine the antibody concentration for this product, since it is ascites, and ascites contains proteins besides the antibody of interest. Ascites fluid is clarified by centrifugation to remove the lipid layer and the cell pellet. It contains specific antibody as well as other host serum proteins including immunoglobulins. While the total protein concentration is ~20 mg/ml, the specific antibody concentration range is 1-10 mg/ml. An exact antibody concentration is only relevant for purified antibodies. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Feb 03 2006

    Question

    Thanks for your reply, but that wasn't exactly what I meant (my fault for not being clearer). What control should be used to test for non-specific binding in my original slides that show binding for ET- 1? e.g. Mouse IgG1? Thanks again

    Read More

    Abcam community

    Verified customer

    Asked on Jun 30 2005

    Answer

    I am very sorry. I misunderstood your question. Yes, using mouse IgG1 will allow you to determine what antibodies found in mouse IgG1 will bind to your slides. If you see some binding then this is background that should not be confused with your ET-1 positive staining. Another control would be to omit the primary antibody so that you can see what non-specific staining is present from the secondary antibody. Finally, since studies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16, a final control would be using the peptide of these amino acids in a blocking experiment. We do not have this peptide available, but it may be commercially available or could be synthesized. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

    Read More

    Abcam Scientific Support

    回复于 Jul 01 2005

    Question

    I want to know your product---antiEndothelin 1 . ab2786 cross reactivity to ET-1 specificity , sensitivity ET2 and ET 3 cross reactivity sensitivity and specificity.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 06 2004

    Answer

    Thank you for your enquiry. Ab2786 detects ET-1 in human tissues. This antibody shows little cross-reactivity to related proteins. The antigen is purified human endothelin-1. Studies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16. We hope this will help,

    Read More

    Abcam Scientific Support

    回复于 Oct 08 2004

    Question

    I went ahead and did a Western with this antibody at a dilution of 1:200. I got a strong band at around 35kDa and some other faint lower and higher molecular weight bands. I expected a band at 24kDa which was absent. I'd appreciate it if your technical team could give suggestions that will improve the blot.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 13 2004

    Answer

    Thank you for your email. Since ab2786 has not yet been tested in Western blotting (to our knowledge), it's tricky to say what could be going on. At this point I would suggest varying the concentrations of both your primary and secondary antibodies, make sure to run a secondary antibody control (no primary), and try running different sample types. Also, make sure to run a positive control. Please let us know how you get on and if you need further information, please contact us again and we will gladly assist you.

    Read More

    Abcam Scientific Support

    回复于 Sep 16 2004

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    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
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