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Cardiovascular Angiogenesis Inhibitors

Anti-Endostatin/COL18A1抗体(ab3453)

  • Datasheet
Submit a review Q&A (2)References (2)

Product price, shipping and contact information

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Immunocytochemistry/ Immunofluorescence - Anti-Endostatin/COL18A1 antibody (ab3453)
  • Immunocytochemistry/ Immunofluorescence - Anti-Endostatin/COL18A1 antibody (ab3453)
  • Immunocytochemistry/ Immunofluorescence - Anti-Endostatin/COL18A1 antibody (ab3453)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endostatin/COL18A1 antibody (ab3453)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endostatin/COL18A1 antibody (ab3453)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endostatin/COL18A1 antibody (ab3453)

Key features and details

  • Rabbit polyclonal to Endostatin/COL18A1
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Mouse, Human, Non human primates
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Endostatin/COL18A1抗体
    参阅全部 Endostatin/COL18A1 一抗
  • 描述

    兔多克隆抗体to Endostatin/COL18A1
  • 宿主

    Rabbit
  • 特异性

    Detects recombinant human Endostatin/COL18A1.

  • 经测试应用

    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Human, Non human primates
  • 免疫原

    Synthetic peptide corresponding to Human Endostatin/COL18A1 aa 129-142.
    Sequence:

    RRLM/TESYCETWRTE


    (Peptide available as ab4983)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    ab3453 has been recombinant only tested in Western blot

     This product was previously labelled as Endostatin

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Angiogenesis
    • Inhibitors
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic inhibitory factors

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Endostatin/COL18A1 protein (ab56290)

应用

Our Abpromise guarantee covers the use of ab3453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200 - 1/1000.
IHC-P 1/100 - 1/500.

靶标

  • 功能

    COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.
    Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.
  • 组织特异性

    Present in multiple organs with highest levels in liver, lung and kidney.
  • 疾病相关

    Defects in COL18A1 are a cause of Knobloch syndrome (KNO) [MIM:267750]. KNO is an autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele.
  • 序列相似性

    Belongs to the multiplexin collagen family.
    Contains 1 FZ (frizzled) domain.
    Contains 1 TSP N-terminal (TSPN) domain.
  • 翻译后修饰

    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • 细胞定位

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P39060 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1306 Human
    • Entrez Gene: 80781 Human
    • Entrez Gene: 12819 Mouse
    • Entrez Gene: 12822 Mouse
    • Omim: 120328 Human
    • SwissProt: P39059 Human
    • SwissProt: P39060 Human
    • SwissProt: O35206 Mouse
    • SwissProt: P39061 Mouse
    • Unigene: 409034 Human
    • Unigene: 517356 Human
    • Unigene: 233547 Mouse
    • Unigene: 4352 Mouse
    see all
  • 别名

    • Alpha 1 collagen type 18 (XVIII)(COL18A1) antibody
    • Alpha 1 type XVIII collagen antibody
    • Antiangiogenic agent antibody
    • COIA1_HUMAN antibody
    • COL15A1 antibody
    • Col18a1 antibody
    • Collagen alpha 1(XV) chain antibody
    • Collagen alpha 1(XVIII) chain antibody
    • Collagen alpha-1(XV) chain antibody
    • Collagen type XV proteoglycan antibody
    • Collagen type XVIII alpha 1 antibody
    • Collagen XV, alpha 1 polypeptide antibody
    • Collagen, type XV, alpha 1 antibody
    • Endostatin antibody
    • Endostatin XV antibody
    • FLJ27325 antibody
    • FLJ34914 antibody
    • FLJ38566 antibody
    • KNO antibody
    • KNO1 antibody
    • KS antibody
    • MGC74745 antibody
    • Multi functional protein MFP antibody
    • OTTHUMP00000021782 antibody
    • OTTHUMP00000115472 antibody
    • OTTHUMP00000115473 antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-Endostatin/COL18A1 antibody (ab3453)
    Immunocytochemistry/ Immunofluorescence - Anti-Endostatin/COL18A1 antibody (ab3453)

    ab3453 labelling Endostatin/COL18A1 (green) in 293T cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorscence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei (blue). Images were taken at a magnification of 60x.

  • Immunocytochemistry/ Immunofluorescence - Anti-Endostatin/COL18A1 antibody (ab3453)
    Immunocytochemistry/ Immunofluorescence - Anti-Endostatin/COL18A1 antibody (ab3453)

    ab3453 labelling Endostatin/COL18A1 (green) in A431 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorscence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei (blue). Images were taken at a magnification of 60x.

  • Immunocytochemistry/ Immunofluorescence - Anti-Endostatin/COL18A1 antibody (ab3453)
    Immunocytochemistry/ Immunofluorescence - Anti-Endostatin/COL18A1 antibody (ab3453)

    ab3453 labelling Endostatin/COL18A1 (green) in NIH-3T3 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorscence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei (blue). Images were taken at a magnification of 60x.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endostatin/COL18A1 antibody (ab3453)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endostatin/COL18A1 antibody (ab3453)

    ab3453 labelling Endostatin/COL18A1 in the secretion of Human kidney tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incuabted with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endostatin/COL18A1 antibody (ab3453)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endostatin/COL18A1 antibody (ab3453)

    ab3453 labelling Endostatin/COL18A1 in the secretion of Human colon tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incuabted with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endostatin/COL18A1 antibody (ab3453)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endostatin/COL18A1 antibody (ab3453)

    ab3453 labelling Endostatin/COL18A1 in the secretion of Mouse kidney tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incuabted with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (2)

    发表研究结果有使用 ab3453?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3453 被引用在 2 文献中.

    • Battinelli EM  et al. Megakaryocytes package contents into separate a-granules that are differentially distributed in platelets. Blood Adv 3:3092-3098 (2019). PubMed: 31648331
    • Mohajeri A  et al. Effect of Culture Condition Variables on Human Endostatin Gene Expression in Escherichia coli Using Response Surface Methodology. Jundishapur J Microbiol 9:e34091 (2016). PubMed: 27800134

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-2 of 2 Abreviews or Q&A

    Question

    You obviously mean protein samples (100ug) right?

    If so it is 100ug and 2ul/ml antibody.

    I use only 25ug protein and 1ug/,l antibody so the ration is reduced by 8 fold.

    That is a big difference.

    I am using 4/15 gradient PAG and run it for an hour at 199V. there is no way I am losing it.

    I have not stained Ponceau S. Maybe I will.

    But so far I do not have any problems with transfer as histone H3 total or GAPDH blots result in abundant signal.


    I didn’t get the replacement policy part.

    Sorry I am sick today and have to leave but can you explain please.

    Aslo, tomorrow I should have another WB finished. We’ll see what happens.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 14 2012

    Answer

    Thank you for your reply.


    Yes, my apologies, the µ symbol did not paste correctly from the protocol. 100 ug were resolved on 10% SDS gels under reducing conditions.


    The recommended working dilution of the antibody is 2ug/ml. You should try incubating with the primary at 2ug/ml overnight at 4C and perhaps increasing the amount of protein loaded if you can.


    If the protocol suggestions do not improve your results, we will replace or refund the product for you.


    I look forward to hearing how today's WB worked and I'm sorry you're not feeling well! We'll get this sorted out for you.

    Read More

    Abcam Scientific Support

    回复于 Mar 14 2012

    Question

    I'm using this antibody in WB using human cell lysate, but not seeing a distinct band.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 13 2012

    Answer

    Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results.

    The lab had tested the below protocol:

    Tumours injected 2 days earlier with angiostatin or empty expression plasmids were excised, minced with scissors, and homogenized in protein lysate buffer [50 mmol /LTris, pH 7.4, 100 mol/L EDTA, 0.25 mol/L sucrose, 1% sodium dodecyl sulfate (SDS), 1% NP40, 1 g/mL leupeptin, 1 g/mL pepstatin A, and 100 mol/L phenylmethylsulfonyl fluoride ] at 48C using a motordriven Virtus homogenizer (Virtus, Gardiner, NY). Tumour lysates from each group of mice were pooled, and protein content was determined. Tissue or cell debris was removed by centrifugation at 10,000g for 10 minutes at 48C. Protein samples (100 g) were resolved on 10% polyacrylamide SDS gels under reducing conditions, and electrophoretically transferred to nitrocellulose Hybond C extra membranes (Amersham Life Science, Buckingham, England, UK). After blocking the membranes with 5% bovine serum albumin in TTBS (Tween 20/Tris - buffered saline 20 mmol /L Tris, 137 mmol/L NaCl, pH 7.6, containing 0.1% Tween-20), blots were incubated with endostatin antibody at 2 ug/ml overnight at 4C. They were then incubated with horseradish peroxidase–conjugated secondary antibody, and developed by enhanced chemiluminescence (Amersham International, Buckingham, England, UK) and exposure to x-ray film. Band density was quantified using Scion.


    You may want to try changing your blocking buffer to 5% BSA instead of milk.


    Also, what gel percentage were you using? It may bebest to try 10% gel if you haven't already since the protein of interest is so small.


    Did you also use a loading control, like beta actin, or check the transfer with Ponceau?


    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

    I hope this information is helpful, and I thank you for your cooperation.

    Read More

    Abcam Scientific Support

    回复于 Mar 13 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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