Anti-EMSY抗体(ab4579)

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Western blot - Anti-EMSY antibody (ab4579)

    Key features and details

    • Goat polyclonal to EMSY
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-EMSY抗体
      参阅全部 EMSY 一抗

    • 描述

      山羊多克隆抗体to EMSY

    • 宿主

      Goat

    • 特异性

      This antibody detects a band of the same size by Western blot as ab123, which was raised with a recombinant fragment of EMSY with no sequence in common with the immunogen for this antibody (and was raised 7 years previous to this antibody). This confirms that the band detected is EMSY.

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Recombinant fragment corresponding to Human EMSY aa 1-100.

    • 阳性对照

      • MCF-7 cells.

    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    应用

    Our Abpromise guarantee covers the use of ab4579 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB 1/500. Predicted molecular weight: 141 kDa.

    靶标

    图片

    • Western blot - Anti-EMSY antibody (ab4579)
      Western blot - Anti-EMSY antibody (ab4579)
      Lanes 1-2 : Anti-EMSY antibody (ab4579) at 1 µg/ml
      Lane 3 : Anti-EMSY antibody (ab123) at 1 µg/ml

      All lanes : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1-2 : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
      Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 141 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 37 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 2 minutes


      Western blot of EMSY on MCF-7 cell lysate.

      Lane 1: ab4579 at 1ug/ml with 2% BSA blocking.

      Lane 2: ab4579 at 1ug/ml with 3% milk blocking.

      Lane 3: ab123 at 1ug/ml with 2% BSA blocking.

      Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD (even though the predicted size is 141kD). However, that the same size band is detected by both ab123 and ab4579 (raised with immunogens of different sequence) confirms the specificity of these antibodies.

       Lane 1 & 2 - Exposure time: 2 min.

      Lane 3 - Exposure time: 2 sec.

    文献 (1)

    发表研究结果有使用 ab4579？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab4579 被引用在 1 文献中.

    • Sand CA  et al. Vascular Expression of Transient Receptor Potential Vanilloid 1 (TRPV1). J Histochem Cytochem 63:449-53 (2015). PubMed: 25809792

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