Anti-EMSY抗体(ab4579)

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Western blot - Anti-EMSY antibody (ab4579)

    Key features and details

    • Goat polyclonal to EMSY
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-EMSY抗体
      参阅全部 EMSY 一抗

    • 描述

      山羊多克隆抗体to EMSY

    • 宿主

      Goat

    • 特异性

      This antibody detects a band of the same size by Western blot as ab123, which was raised with a recombinant fragment of EMSY with no sequence in common with the immunogen for this antibody (and was raised 7 years previous to this antibody). This confirms that the band detected is EMSY.

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Recombinant fragment corresponding to Human EMSY aa 1-100.

    • 阳性对照

      • MCF-7 cells.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab4579于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/500. Predicted molecular weight: 141 kDa.
    说明
    WB
    1/500. Predicted molecular weight: 141 kDa.

    靶标

    图片

    • Western blot - Anti-EMSY antibody (ab4579)
      Western blot - Anti-EMSY antibody (ab4579)
      Lanes 1-2 : Anti-EMSY antibody (ab4579) at 1 µg/ml
      Lane 3 : Anti-EMSY antibody (ab123) at 1 µg/ml

      All lanes : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1-2 : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
      Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 141 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?
      Additional bands at: 37 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 2 minutes


      Western blot of EMSY on MCF-7 cell lysate.

      Lane 1: ab4579 at 1ug/ml with 2% BSA blocking.

      Lane 2: ab4579 at 1ug/ml with 3% milk blocking.

      Lane 3: ab123 at 1ug/ml with 2% BSA blocking.

      Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD (even though the predicted size is 141kD). However, that the same size band is detected by both ab123 and ab4579 (raised with immunogens of different sequence) confirms the specificity of these antibodies.

       Lane 1 & 2 - Exposure time: 2 min.

      Lane 3 - Exposure time: 2 sec.

    文献 (1)

    发表研究结果有使用 ab4579？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab4579 被引用在 1 文献中.

    • Sand CA  et al. Vascular Expression of Transient Receptor Potential Vanilloid 1 (TRPV1). J Histochem Cytochem 63:449-53 (2015). PubMed: 25809792

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