Key features and details
- Goat polyclonal to EMSY
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
参阅全部 EMSY 一抗
特异性This antibody detects a band of the same size by Western blot as ab123, which was raised with a recombinant fragment of EMSY with no sequence in common with the immunogen for this antibody (and was raised 7 years previous to this antibody). This confirms that the band detected is EMSY.
经测试应用适用于: WBmore details
Recombinant fragment corresponding to Human EMSY aa 1-100.
- MCF-7 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.025% Sodium azide
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab4579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 141 kDa.|
- Information by UniProt
- C11orf30 antibody
- Chromosome 11 open reading frame 30 antibody
- Emsy antibody
Lanes 1-2 : Anti-EMSY antibody (ab4579) at 1 µg/ml
Lane 3 : Anti-EMSY antibody (ab123) at 1 µg/ml
All lanes : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Lanes 1-2 : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 141 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Western blot of EMSY on MCF-7 cell lysate.
Lane 1: ab4579 at 1ug/ml with 2% BSA blocking.
Lane 2: ab4579 at 1ug/ml with 3% milk blocking.
Lane 3: ab123 at 1ug/ml with 2% BSA blocking.
Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD (even though the predicted size is 141kD). However, that the same size band is detected by both ab123 and ab4579 (raised with immunogens of different sequence) confirms the specificity of these antibodies.
Lane 1 & 2 - Exposure time: 2 min.
Lane 3 - Exposure time: 2 sec.
ab4579 被引用在 1 文献中.
- Sand CA et al. Vascular Expression of Transient Receptor Potential Vanilloid 1 (TRPV1). J Histochem Cytochem 63:449-53 (2015). PubMed: 25809792