重组Anti-ELMO1抗体[EPR12919] (ab174298)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12919] to ELMO1
- Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ELMO1抗体[EPR12919]
参阅全部 ELMO1 一抗 -
描述
兔单克隆抗体[EPR12919] to ELMO1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IP, Flow Cyt (Intra)more details
不适用于: IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12919 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab174298于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/100 - 1/250.
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WB |
1/10000 - 1/50000. Predicted molecular weight: 84 kDa.
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IP |
1/10 - 1/100.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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说明 |
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ICC/IF
1/100 - 1/250. |
WB
1/10000 - 1/50000. Predicted molecular weight: 84 kDa. |
IP
1/10 - 1/100. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
靶标
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功能
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. -
组织特异性
Widely expressed, with a higher expression in the spleen and placenta. -
序列相似性
Contains 1 ELMO domain.
Contains 1 PH domain. -
翻译后修饰
Phosphorylated by HCK. -
细胞定位
Cytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK. - Information by UniProt
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数据库链接
- Entrez Gene: 9844 Human
- Entrez Gene: 140580 Mouse
- Entrez Gene: 361251 Rat
- Omim: 606420 Human
- SwissProt: Q92556 Human
- SwissProt: Q8BPU7 Mouse
- Unigene: 656638 Human
- Unigene: 342392 Mouse
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别名
- CED 12 antibody
- Ced 12 homolog 1 antibody
- Ced 12 homolog antibody
see all
图片
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All lanes : Anti-ELMO1 antibody [EPR12919] (ab174298) at 1/10000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 84 kDa -
Immunocytochemistry/ Immunofluorescence analysis of K562 labeling ELMO1 with ab174298 at 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor® 488); ab150077 at 1/1000 was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. DAPI was used to counter stain nuclei (blue).
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LR73 cells were transfected with the indicated plasmids and stained with Alexa Fluor 488–conjugated phalloidin and anti-Elmo1 antibody.
One day after transfection, cells were washed with cold PBS, fixed in 4% paraformaldehyde for 15 min, and permeabilized with 0.1% Triton X-100 for 5 min. Next, permeabilized cells were blocked with 1% BSA for 30 min and stained with Alex Fluor 594–conjugated phalloidin and anti-Elmo1 antibody for 1 h at room temperature.
(After Figure 5 of Kim et al).
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Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling ELMO1 with unpurified ab174298 at 1/200 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab174298 at 1/10 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab174298 被引用在 2 文献中.
- Liang Y et al. Downregulation of Dock1 and Elmo1 suppresses the migration and invasion of triple-negative breast cancer epithelial cells through the RhoA/Rac1 pathway. Oncol Lett 16:3481-3488 (2018). PubMed: 30127952
- Kim K et al. Intermolecular steric inhibition of Ephexin4 is relieved by Elmo1. Sci Rep 7:4404 (2017). PubMed: 28667327