Anti-ELMO1抗体- C-terminal (ab155775)
Key features and details
- Rabbit polyclonal to ELMO1 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ELMO1抗体- C-terminal
参阅全部 ELMO1 一抗 -
描述
兔多克隆抗体to ELMO1 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Chicken, Cow, Zebrafish -
免疫原
Synthetic peptide, corresponding to a region within C terminal amino acids 664-727 of Human ELMO1 (Uniprot ID Q92556).
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阳性对照
- H1299 whole cell lysate; HeLa cells; HepG2 and A431 cell line; Human Stomach tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab155775于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 84 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 84 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. -
组织特异性
Widely expressed, with a higher expression in the spleen and placenta. -
序列相似性
Contains 1 ELMO domain.
Contains 1 PH domain. -
翻译后修饰
Phosphorylated by HCK. -
细胞定位
Cytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK. - Information by UniProt
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数据库链接
- Entrez Gene: 420735 Chicken
- Entrez Gene: 509821 Cow
- Entrez Gene: 9844 Human
- Entrez Gene: 140580 Mouse
- Entrez Gene: 361251 Rat
- Entrez Gene: 406364 Zebrafish
- Omim: 606420 Human
- SwissProt: Q92556 Human
see all -
别名
- CED 12 antibody
- Ced 12 homolog 1 antibody
- Ced 12 homolog antibody
see all
图片
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Anti-ELMO1 antibody - C-terminal (ab155775) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 84 kDa
12% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa, labeling ELMO1 with ab155775 at 1/500 dilution. Blue: Hoechst 33342 staining.
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Immunohistochemical analysis of paraffin-embedded Human Stomach tissue, labeling ELMO1 with ab155775 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab155775 被引用在 2 文献中.
- Xue R et al. Functional Verification of Novel ELMO1 Variants by Live Imaging in Zebrafish. Front Cell Dev Biol 9:723804 (2021). PubMed: 34993193
- Shi L et al. CC chemokine ligand 18(CCL18) promotes migration and invasion of lung cancer cells by binding to Nir1 through Nir1-ELMO1/DOC180 signaling pathway. Mol Carcinog 55:2051-2062 (2016). PubMed: 26756176