Anti-EKLF / KLF1抗体(ab2483)
Key features and details
- Goat polyclonal to EKLF / KLF1
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-EKLF / KLF1抗体
参阅全部 EKLF / KLF1 一抗 -
描述
山羊多克隆抗体to EKLF / KLF1该产品是 快线抗体，通过亲和纯化得到。对抗原肽有高灵敏度及特异性（ELISA测试）。 Read the terms of use »
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宿主
Goat -
种属反应性
与反应: Mouse, Human
预测可用于: Cow, Dog, Pig
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免疫原
Synthetic peptide:
ATAETALPSISTLT
, corresponding to N terminal amino acids 2-15 of Human KLF1.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
靶标
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功能
Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation. -
组织特异性
Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines. -
疾病相关
Defects in KLF1 are the cause of congenital dyserythropoietic anemia type 4 (CDA4) [MIM:613673]. It is a blood disorder characterized by ineffective erythropoiesis and hemolysis resulting in anemia. Circulating erythroblasts and erythroblasts in the bone marrow show various morphologic abnormalities. Affected individuals with CDA4 also have increased levels of fetal hemoglobin. -
序列相似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
翻译后修饰
Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity.
Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex.
Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity. -
细胞定位
Nucleus. Colocalizes with SUMO1 in nuclear speckles. - Information by UniProt
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数据库链接
- Entrez Gene: 10661 Human
- Entrez Gene: 16596 Mouse
- Omim: 600599 Human
- SwissProt: Q13351 Human
- SwissProt: P46099 Mouse
- Unigene: 37860 Human
- Unigene: 4847 Mouse
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别名
- CDAN4 antibody
- EKLF antibody
- EKLF PEN antibody
see all
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
文献 (13)
ab2483 被引用在 13 文献中.
- Fanis P et al. A novel mutation in the erythroid transcription factor KLF1 is likely responsible for ameliorating ß-thalassemia major. Hum Mutat 40:1768-1780 (2019). PubMed: 31115947
- Nakagawa MM & Rathinam CV Constitutive Activation of the Canonical NF-?B Pathway Leads to Bone Marrow Failure and Induction of Erythroid Signature in Hematopoietic Stem Cells. Cell Rep 25:2094-2109.e4 (2018). PubMed: 30463008
- Chennupati V et al. Ribonuclease inhibitor 1 regulates erythropoiesis by controlling GATA1 translation. J Clin Invest 128:1597-1614 (2018). PubMed: 29408805
- Li Y et al. MicroRNA 200a inhibits erythroid differentiation by targeting PDCD4 and THRB. Br J Haematol 176:50-64 (2017). PubMed: 27734462
- Kim YW & Kim A Deletion of transcription factor binding motifs using the CRISPR/spCas9 system in the ß-globin LCR. Biosci Rep N/A:N/A (2017). PubMed: 28729249
