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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger

Anti-EKLF / KLF1抗体(ab2483)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (14)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EKLF / KLF1 antibody (ab2483)

    Key features and details

    • Goat polyclonal to EKLF / KLF1
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-EKLF / KLF1抗体
      参阅全部 EKLF / KLF1 一抗
    • 描述

      山羊多克隆抗体to EKLF / KLF1
    • 宿主

      Goat
    • 经测试应用

      适用于: IHC-Pmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      Synthetic peptide within Human EKLF/ KLF1 aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 阳性对照

      • IHC-P: Human bone marrow tissue.
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Zinc Finger
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Krueppel like factor
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Krueppel like factor

    相关产品

    • ChIP Related Products

      • ChIP Kit (ab500)
    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab2483于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    说明
    IHC-P
    Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    靶标

    • 功能

      Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.
    • 组织特异性

      Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines.
    • 疾病相关

      Defects in KLF1 are the cause of congenital dyserythropoietic anemia type 4 (CDA4) [MIM:613673]. It is a blood disorder characterized by ineffective erythropoiesis and hemolysis resulting in anemia. Circulating erythroblasts and erythroblasts in the bone marrow show various morphologic abnormalities. Affected individuals with CDA4 also have increased levels of fetal hemoglobin.
    • 序列相似性

      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 3 C2H2-type zinc fingers.
    • 翻译后修饰

      Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity.
      Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex.
      Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity.
    • 细胞定位

      Nucleus. Colocalizes with SUMO1 in nuclear speckles.
    • Target information above from: UniProt accession Q13351 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 10661 Human
      • Omim: 600599 Human
      • SwissProt: Q13351 Human
      • Unigene: 37860 Human
      • 别名

        • CDAN4 antibody
        • EKLF antibody
        • EKLF PEN antibody
        • Erythroid krueppel like transcription factor (EKLF) (Erythroid transcription factor) antibody
        • Erythroid krueppel-like transcription factor antibody
        • Erythroid Kruppel like factor antibody
        • Erythroid specific transcription factor EKLF antibody
        • HBFQTL6 antibody
        • Human erythroid-specific transcription factor EKLF mRNA complete cds antibody
        • INLU antibody
        • Klf1 antibody
        • KLF1_HUMAN antibody
        • Krueppel-like factor 1 antibody
        • Kruppel like factor 1 (erythroid) antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EKLF / KLF1 antibody (ab2483)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EKLF / KLF1 antibody (ab2483)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bone marrow tissue labeling EKLF / KLF1 with ab2483 at 4 µg/mL. Heat mediated antigen retrieval was performed using citrate buffer pH 6. HRP staining.

      实验方案

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (14)

      发表研究结果有使用 ab2483?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab2483 被引用在 14 文献中.

      • Fanis P  et al. A novel mutation in the erythroid transcription factor KLF1 is likely responsible for ameliorating ß-thalassemia major. Hum Mutat 40:1768-1780 (2019). PubMed: 31115947
      • Kuppers DA  et al. N6-methyladenosine mRNA marking promotes selective translation of regulons required for human erythropoiesis. Nat Commun 10:4596 (2019). PubMed: 31601799
      • Chennupati V  et al. Ribonuclease inhibitor 1 regulates erythropoiesis by controlling GATA1 translation. J Clin Invest 128:1597-1614 (2018). PubMed: 29408805
      • Nakagawa MM & Rathinam CV Constitutive Activation of the Canonical NF-?B Pathway Leads to Bone Marrow Failure and Induction of Erythroid Signature in Hematopoietic Stem Cells. Cell Rep 25:2094-2109.e4 (2018). PubMed: 30463008
      • Li Y  et al. MicroRNA 200a inhibits erythroid differentiation by targeting PDCD4 and THRB. Br J Haematol 176:50-64 (2017). PubMed: 27734462
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-2 of 2 Abreviews or Q&A

      Question

      However, we can't be sure particularly the specificity of EKFL antibody.

      Furthermore, we can't understand the figure (ChIP Grade image) described in the website:
      https://www.abcam.com/EKLF-KLF1-antibody-ChIP-Grade-ab2483.html#EKLF-KLF1-Primary-antibodies-ab2483-3.jpg
      Why it says FOXP3 antibody for ChIP on the top?
      We are afraid of the one we purchased is really specific for EKLF-1.
      Could you give us any advise?
      Our samples are really previous
      We'd like to make sure and save "time/money" as well.
      Thanks for your attention.

      Read More

      Abcam community

      Verified customer

      Asked on Apr 16 2012

      Answer

      Thank you for contacting Abcam.

      I have looked in this and it appears that the wrong image was placed on the Webpage for ab2483, however we do guarantee the antibody to work in ChIP, as the correct image can be found at the link below:

      https://www.abcam.com/EKLF--KLF1-antibody-ab2483.html&tab=abreviews&intabreviewid=14213

      Also we have 2 publications that have successfully used this antibody in ChIP:

      https://www.abcam.com/EKLF-KLF1-antibody-ChIP-Grade-ab2483-references.html

      I am sorry about this confusion and I will have it sorted as soon as possible but you can be confident that the antibody is covered under our Abpromise to work in ChiP.

      If there is anything else I can help you with, please let me know.

      Read More

      Abcam Scientific Support

      回复于 Apr 17 2012

      ChIP abreview for Anti-EKLF / KLF1 antibody - ChIP Grade

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      ChIP
      Detection step
      Real-time PCR
      Sample
      Human Cell lysate - nuclear (CD34+ cells)
      Specification
      CD34+ cells
      Negative control
      Region on chromosome 5 (Untr5) that is far from any known gene annotation and not expected to be bound by KLF1
      Type
      Cross-linking (X-ChIP)
      Duration of cross-linking step: 15 minute(s) and 0 second(s)
      Specification of the cross-linking agent: Formaldehyde
      Positive control
      Region upstream of the transcription start site of the HBE1 gene
      Read More

      Mr. Genpathway Inc

      Verified customer

      提交于 Jul 29 2009

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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