Anti-EKLF / KLF1抗体(ab2483)
Key features and details
- Goat polyclonal to EKLF / KLF1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-EKLF / KLF1抗体
参阅全部 EKLF / KLF1 一抗 -
描述
山羊多克隆抗体to EKLF / KLF1 -
宿主
Goat -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human EKLF/ KLF1 aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- IHC-P: Human bone marrow tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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IHC-P
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation. -
组织特异性
Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines. -
疾病相关
Defects in KLF1 are the cause of congenital dyserythropoietic anemia type 4 (CDA4) [MIM:613673]. It is a blood disorder characterized by ineffective erythropoiesis and hemolysis resulting in anemia. Circulating erythroblasts and erythroblasts in the bone marrow show various morphologic abnormalities. Affected individuals with CDA4 also have increased levels of fetal hemoglobin. -
序列相似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
翻译后修饰
Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity.
Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex.
Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity. -
细胞定位
Nucleus. Colocalizes with SUMO1 in nuclear speckles. - Information by UniProt
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数据库链接
- Entrez Gene: 10661 Human
- Omim: 600599 Human
- SwissProt: Q13351 Human
- Unigene: 37860 Human
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别名
- CDAN4 antibody
- EKLF antibody
- EKLF PEN antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EKLF / KLF1 antibody (ab2483)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bone marrow tissue labeling EKLF / KLF1 with ab2483 at 4 µg/mL. Heat mediated antigen retrieval was performed using citrate buffer pH 6. HRP staining.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (14)
ab2483 被引用在 14 文献中.
- Fanis P et al. A novel mutation in the erythroid transcription factor KLF1 is likely responsible for ameliorating ß-thalassemia major. Hum Mutat 40:1768-1780 (2019). PubMed: 31115947
- Kuppers DA et al. N6-methyladenosine mRNA marking promotes selective translation of regulons required for human erythropoiesis. Nat Commun 10:4596 (2019). PubMed: 31601799
- Chennupati V et al. Ribonuclease inhibitor 1 regulates erythropoiesis by controlling GATA1 translation. J Clin Invest 128:1597-1614 (2018). PubMed: 29408805
- Nakagawa MM & Rathinam CV Constitutive Activation of the Canonical NF-?B Pathway Leads to Bone Marrow Failure and Induction of Erythroid Signature in Hematopoietic Stem Cells. Cell Rep 25:2094-2109.e4 (2018). PubMed: 30463008
- Li Y et al. MicroRNA 200a inhibits erythroid differentiation by targeting PDCD4 and THRB. Br J Haematol 176:50-64 (2017). PubMed: 27734462
