eIF5A2 overexpression 293T裂解物(whole cell) (ab94210)
概述
-
产品名称
eIF5A2 overexpression 293T裂解物(whole cell) -
常规说明
ab94210 is a 293T cell transfected lysate in which Human eIF5A2 has been transiently over-expressed using a pCMV-eIF5A2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
-
经测试应用
适用于: WBmore details
性能
-
Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
研究领域
-
背景
Function: mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Tissue specificity: Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells. Similarity: Belongs to the eIF-5A family. PTM: eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94210于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use at an assay dependent dilution.
|
说明 |
---|
WB
Use at an assay dependent dilution. |
图片
-
ab94210 at 15μg/lane on an SDS-PAGE gel
-
All lanes : Anti-eIF5A2 antibody (ab57421) at 1/500 dilution
Lane 1 :eIF5A2 overexpression 293T lysate (whole cell) (ab94210)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab94210 尚未被引用在任何文献中。