重组Anti-eIF5A2 + eIF5A抗体[EPR7412-47] (ab126733)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7412-47] to eIF5A2 + eIF5A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-eIF5A2 + eIF5A抗体[EPR7412-47]
参阅全部 eIF5A2 + eIF5A 一抗 -
描述
兔单克隆抗体[EPR7412-47] to eIF5A2 + eIF5A -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human eIF5A2 + eIF5A aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary. The immunogen sequence is based upon a region of eIF5A2 that has high homology with eIF5A.
Database link: Q9GZV4 -
阳性对照
- HepG2 and Caco 2 cell lysates; Human ovarian carcinoma tissue; HepG2 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 2.11 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7412-47 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126733于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
靶标
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细胞定位
eIF5A2: Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions. eIF5A: Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions. -
数据库链接
- Entrez Gene: 1984 Human
- Entrez Gene: 56648 Human
- Entrez Gene: 208691 Mouse
- Entrez Gene: 276770 Mouse
- Entrez Gene: 287444 Rat
- Entrez Gene: 310261 Rat
- Omim: 600187 Human
- Omim: 605782 Human
see all
图片
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All lanes : Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 17 kDa -
ab126733 at 1/100 dilution, staining eIF5A2 + eIF5A in Formalin-fixed Paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab126733, at 1/100 dilution, staining eIF5A2 + eIF5A in HepG2 cells by Immunofluorescence (green). DAPI counterstain (blue).
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All lanes : Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733) at 1/1000 dilution
Lane 1 :Recombinant Human eIF5A protein (ab87457)
Lane 2 :Recombinant Human eIF5A2 protein (ab99140)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 17 kDa
Observed band size: 15,20 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking buffer: 5% NFDM/TBST
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab126733 被引用在 1 文献中.
- Xu G et al. Eukaryotic initiation factor 5A2 mediates hypoxia-induced autophagy and cisplatin resistance. Cell Death Dis 13:683 (2022). PubMed: 35931669