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Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation

Anti-eIF4EBP1抗体(ab2606)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (25)

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Western blot - Anti-eIF4EBP1 antibody (ab2606)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 antibody (ab2606)
  • Western blot - Anti-eIF4EBP1 antibody (ab2606)
  • Western blot - Anti-eIF4EBP1 antibody (ab2606)
  • Western blot - Anti-eIF4EBP1 antibody (ab2606)

Key features and details

  • Rabbit polyclonal to eIF4EBP1
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human, Pig
  • Isotype: IgG

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概述

  • 产品名称

    Anti-eIF4EBP1抗体
    参阅全部 eIF4EBP1 一抗
  • 描述

    兔多克隆抗体to eIF4EBP1
  • 宿主

    Rabbit
  • 特异性

    This antibody detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1.
  • 经测试应用

    适用于: IHC-P, WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Human, Pig
    预测可用于: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide (Human) - which represents a portion of human eIF-4EBP1 encoded in part by exons 2and 3.

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 纯化说明

    Affinity purified using the immunising peptideimmobilized on solid support.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Metabolic disorders

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human eIF4EBP1 protein (ab60007)

应用

Our Abpromise guarantee covers the use of ab2606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/5000 - 1/20000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.

靶标

  • 功能

    Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
  • 序列相似性

    Belongs to the eIF4E-binding protein family.
  • 翻译后修饰

    Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Target information above from: UniProt accession Q13541 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1978 Human
    • Entrez Gene: 13685 Mouse
    • Entrez Gene: 100627508 Pig
    • Entrez Gene: 116636 Rat
    • Omim: 602223 Human
    • SwissProt: Q13541 Human
    • SwissProt: Q60876 Mouse
    • SwissProt: Q62622 Rat
    • Unigene: 411641 Human
    • Unigene: 6700 Mouse
    • Unigene: 11161 Rat
    see all
  • 别名

    • 4E-BP1 antibody
    • 4EBP1 antibody
    • 4EBP1_HUMAN antibody
    • BP 1 antibody
    • eIF4E binding protein 1 antibody
    • eIF4E-binding protein 1 antibody
    • Eif4ebp1 antibody
    • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
    • PHAS-I antibody
    • PHASI antibody
    • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
    see all

图片

  • Western blot - Anti-eIF4EBP1 antibody (ab2606)
    Western blot - Anti-eIF4EBP1 antibody (ab2606)

    Ab2606 at a 1:4000 dilution staining alpha, beta and gamma isoforms of  eIF-4EBP1 in 100µg of RIN-m5F (Rat insulinoma cell line) whole cell lysate
    after exposure to high (lane 1) and low (lane 2) concentrations of leucine. Ab2606 at a 1:4000 dilution staining alpha, beta and gamma isoforms of eIF-4EBP1 in 100µg of RINm5F cell lysate after exposure to high (lane 1) and low (lane 2) concentrations of leucine.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 antibody (ab2606)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 antibody (ab2606)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling eIF4EBP1 with ab2606 at 1/20000 (0.05µg/ml). Detection: DAB.
  • Western blot - Anti-eIF4EBP1 antibody (ab2606)
    Western blot - Anti-eIF4EBP1 antibody (ab2606)This image is a courtesy of Anonymous Abreview
    Anti-eIF4EBP1 antibody (ab2606) at 1/500 dilution + Lysate prepared from human HT1080 cell line at 10 µg

    Secondary
    HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 13 kDa
    Observed band size: 20,22,25 kDa
    why is the actual band size different from the predicted?


    Exposure time: 12 minutes

    See Abreview

  • Western blot - Anti-eIF4EBP1 antibody (ab2606)
    Western blot - Anti-eIF4EBP1 antibody (ab2606)This image is a courtesy of Anonymous Abreview
    Anti-eIF4EBP1 antibody (ab2606) at 1/500 dilution + Lysate prepared from mouse NIH 3T3 cells at 10 µg

    Secondary
    HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 13 kDa
    Observed band size: 22,25 kDa why is the actual band size different from the predicted?


    Exposure time: 12 minutes

    See Abreview

  • Western blot - Anti-eIF4EBP1 antibody (ab2606)
    Western blot - Anti-eIF4EBP1 antibody (ab2606)
    All lanes : Anti-eIF4EBP1 antibody (ab2606) at 1/1000 dilution

    Lane 1 : Recombinant Human eIF4EBP1 protein (ab60007) at 0.01 µg
    Lane 2 : Recombinant Human eIF4EBP1 protein (ab60007) at 0.001 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 13 kDa


    Exposure time: 10 seconds

实验方案

  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (25)

    发表研究结果有使用 ab2606?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2606 被引用在 25 文献中.

    • Tian Y  et al. Silencing of RHEB inhibits cell proliferation and promotes apoptosis in colorectal cancer cells via inhibition of the mTOR signaling pathway. J Cell Physiol 235:442-453 (2020). PubMed: 31332784
    • Wang M  et al. Rapamycin suppresses angiogenesis and lymphangiogenesis in melanoma by downregulating VEGF-A/VEGFR-2 and VEGF-C/VEGFR-3 expression. Onco Targets Ther 12:4643-4654 (2019). PubMed: 31354297
    • Wang Q  et al. The mTOR Signal Pathway Is Overactivated in Human Lymphatic Malformations. Lymphat Res Biol N/A:N/A (2019). PubMed: 31381473
    • Guo Z  et al. mTORC2 Regulates Lipogenic Gene Expression through PPAR? to Control Lipid Synthesis in Bovine Mammary Epithelial Cells. Biomed Res Int 2019:5196028 (2019). PubMed: 31223619
    • Ma YF  et al. Phosphorylation of AKT serine/threonine kinase and abundance of milk protein synthesis gene networks in mammary tissue in response to supply of methionine in periparturient Holstein cows. J Dairy Sci 102:4264-4274 (2019). PubMed: 30879806
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-eIF4EBP1 antibody

    Average
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: CC1 Antigen Retrieval Buffer
    Permeabilization
    No
    Specification
    liver
    Blocking step
    Rodent block M as blocking agent for 12 minute(s) · Concentration: 100% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 07 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-eIF4EBP1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Vascular smooth muscle cell)
    Permeabilization
    Yes - NP40
    Specification
    Vascular smooth muscle cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 20 2018

    Western blot abreview for Anti-eIF4EBP1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (NIH 3T3 cells)
    Loading amount
    10 µg
    Specification
    NIH 3T3 cells
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 18 2009

    Western blot abreview for Anti-eIF4EBP1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HT1080 cell line)
    Loading amount
    10 µg
    Specification
    HT1080 cell line
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 18 2009

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