Anti-eIF4E抗体(ab1126)
Key features and details
- Rabbit polyclonal to eIF4E
- Suitable for: WB, IHC-P
- Reacts with: Cow, Human
- Isotype: IgG
概述
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产品名称
Anti-eIF4E抗体
参阅全部 eIF4E 一抗 -
描述
兔多克隆抗体to eIF4E -
宿主
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Cow -
免疫原
Recombinant full length protein (Human).
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
---|---|
IHC-P |
Human
|
WB |
Cow
|
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
|
IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
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WB
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. -
序列相似性
Belongs to the eukaryotic initiation factor 4E family. -
翻译后修饰
Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex. - Information by UniProt
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数据库链接
- Entrez Gene: 281751 Cow
- Entrez Gene: 1977 Human
- Omim: 133440 Human
- SwissProt: Q9N0T5 Cow
- SwissProt: P06730 Human
- Unigene: 249718 Human
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别名
- AUTS19 antibody
- CBP antibody
- eIF 4E antibody
see all
图片
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Anti-eIF4E antibody (ab1126) + 30ug bovine kidney extract
Predicted band size: 25 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Western blot of 30µ g bovine kidney extract with anti-eIF-4E (GLO153). The arrow denotes the position corresponding to eIF-4E (~ 28 kDa). -
IHC image of ab1126 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1126, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
文献 (13)
ab1126 被引用在 13 文献中.
- Batool A et al. Eukaryotic Initiation Factor 4E phosphorylation acts a switch for its binding to 4E-BP1 and mRNA cap assembly. Biochem Biophys Res Commun 527:489-495 (2020). PubMed: 32336547
- Pitchiaya S et al. Dynamic Recruitment of Single RNAs to Processing Bodies Depends on RNA Functionality. Mol Cell 74:521-533.e6 (2019). PubMed: 30952514
- Sun Y et al. Newcastle disease virus induces stable formation of bona fide stress granules to facilitate viral replication through manipulating host protein translation. FASEB J 31:1337-1353 (2017). PubMed: 28011649
- Schunter AJ et al. Phosphoproteomics of colon cancer metastasis: comparative mass spectrometric analysis of the isogenic primary and metastatic cell lines SW480 and SW620. Anal Bioanal Chem 409:1749-1763 (2017). PubMed: 27987026
- Gujrati M et al. Targeted Dual pH-Sensitive Lipid ECO/siRNA Self-Assembly Nanoparticles Facilitate In Vivo Cytosolic sieIF4E Delivery and Overcome Paclitaxel Resistance in Breast Cancer Therapy. Adv Healthc Mater 5:2882-2895 (2016). PubMed: 27723260