概述

  • 产品名称
    Anti-eIF4E抗体
    参阅全部 eIF4E 一抗
  • 描述
    兔多克隆抗体to eIF4E
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IP, WB, IHC-P, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human).

性能

应用

Our Abpromise guarantee covers the use of ab1126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP 1/1000.
WB 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/1000.
ICC/IF Use at an assay dependent concentration. See Abreview.

靶标

  • 功能
    Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
  • 序列相似性
    Belongs to the eukaryotic initiation factor 4E family.
  • 翻译后修饰
    Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
  • Information by UniProt
  • 数据库链接
    • 别名
      • AUTS19 antibody
      • CBP antibody
      • eIF 4E antibody
      • eIF 4F 25 kDa subunit antibody
      • EIF 4F antibody
      • eIF-4E antibody
      • eIF-4F 25 kDa subunit antibody
      • eIF4E antibody
      • EIF4E1 antibody
      • EIF4EL1 antibody
      • EIF4F antibody
      • Eukaryotic translation initiation factor 4 E antibody
      • Eukaryotic translation initiation factor 4E antibody
      • Eukaryotic translation initiation factor 4E like 1 antibody
      • IF4E_HUMAN antibody
      • Messanger RNA Cap Binding Protein eIF 4E antibody
      • MGC111573 antibody
      • mRNA cap binding protein antibody
      • mRNA cap-binding protein antibody
      see all

    图片

    • Western blot - Anti-eIF4E antibody (ab1126)
      Western blot - Anti-eIF4E antibody (ab1126)
      Anti-eIF4E antibody (ab1126) + 30ug bovine kidney extract

      Predicted band size: 25 kDa
      Observed band size: 28 kDa (why is the actual band size different from the predicted?)



       Western blot of 30 µg bovine kidney extract with anti-eIF-4E (GLO153). The arrow denotes the position corresponding to eIF-4E (~ 28 kDa).
    • Immunocytochemistry/ Immunofluorescence - Anti-eIF4E antibody (ab1126)
      Immunocytochemistry/ Immunofluorescence - Anti-eIF4E antibody (ab1126)This image is courtesy of an anonymous Abreview

      ab1126 at 1/100 staining human epithelial cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 1 hour. An Alexa-Fluor ® 647 conjugated goat anti-rabbit IgG was used as the secondary.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4E antibody (ab1126)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4E antibody (ab1126)
      IHC image of ab1126 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1126, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    文献

    This product has been referenced in:
    • Zhang P  et al. p53, MDM2, eIF4E and EGFR expression in nasopharyngeal carcinoma and their correlation with clinicopathological characteristics and prognosis: A retrospective study. Oncol Lett 9:113-118 (2015). IHC ; Human . Read more (PubMed: 25435943) »
    • Chen B  et al. Knockdown of eukaryotic translation initiation factor 4E suppresses cell growth and invasion, and induces apoptosis and cell cycle arrest in a human lung adenocarcinoma cell line. Mol Med Rep 12:7971-8 (2015). WB . Read more (PubMed: 26498338) »

    See all 7 Publications for this product

    发表研究结果有使用 ab1126？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    10 reviews or Q&A

    10
    Votes: Highest First

    Thank you for your email. The issue for AB1126 is for the same customer. This started from August 2011.

    This customer did receive a replacement of 1 vial form the same Lot# but initially he ordered 2, and this also failed for him.

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    Thank you for your response.
    This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of two vials of ab1126. For your information, the internal reference note for this credi...

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    Dear Technical team Good day! We have a customer who has purchased the above antibody and would like to know the following information: As your website recommended the tissue and dilution of antibody I have done work like that. Process: An...

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    Thank you for contacting us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. As it can be seen from the image in the datasheet, human breast carcinoma is a good option for a positive control. Howev...

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    Western blot abreview for Anti-eIF4E antibody

     Average
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLA)
    Loading amount
    20 µg
    Specification
    HeLA
    Gel Running Conditions
    Reduced Denaturing (15% Tris-tricine gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    提交于 Sep 06 2011

    Dear Technical,   Good day! I was wondering if you could give us a recommended dilution for IHC-P for this antibody. As the recommended dilution states to use 1ug/ml but it is a bit difficult when the concentration of the antibody is not recommended...

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    Thank you for your enquiry regarding ab1126. I can confirm that the antibody has been tested in IHC-P at a dilution of 1/1000 (instead of 1ug/ml). The datasheet (application notes) and the legend of the IHC-P image has been amended accordingly. ...

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-eIF4E antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Epithelial)
    Specification
    Epithelial
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton-X 100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    提交于 Jun 05 2007

    I have purchased a number of antibodies from Abcam and they have all been good. I have couple of questions which i would like you to assist me with, as i have checked your website and found no answers. The Rabbit polyclonal eIF4E antibody (ab1126...

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    Thank you for your enquiry. I can confirm that both these eIF2a antibodies (ab1126, ab5369)will detect both phosphorylated and de-phosphorylated forms of the protein. I hope this information is helpful. Should you have any further questions, pl...

    Read More

    Immunocytochemistry/ Immunofluorescence abreview for Anti-eIF4E antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Blocking step
    1% BSA + 1.5% normal goat serum as blocking agent for 30 minute(s) · Concentration: 1
    Read More
    Username

    Dr. Karppiah Muthumani

    Verified customer

    提交于 Nov 17 2006

    Western blot abreview for Anti-eIF4E antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (HeLa)
    Loading amount
    50 µg
    Specification
    HeLa
    Treatment
    rVpr & LMB
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
    Read More
    Username

    Dr. Karppiah Muthumani

    Verified customer

    提交于 Nov 17 2006

    Thanks for your rapid reply, it was a misunderstandig. Of course we know, that eIF4E is well expressed in HeLa. and we used for eIF4E a little aliquot of an ab which we got as a present from another lab. So previously we could detect eIF4E. The point ...

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    Thank you very much for your e-mail. I would like to reassure you that if an antibody does not perform as specified on the datasheet, we will provide a full refund or offer you a replacement of equivalent value. I have contacted the supplier reg...

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    I have used the anti-eIF4E FROM NEB-CST, which although recommended for immunoprecipitation does not work for this application in my hands. Is your antibody definitely capable of immunoprecipitating from cell lysates and not just purified protein? Is...

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    Yes, our antibody is capable of precipitating eIF-4E from cell lysates. The antibody was made to the full length recombinant human protein.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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