Anti-eIF4B (phospho S504)抗体[EP2261Y] (ab75823)
Key features and details
- Rabbit monoclonal [EP2261Y] to eIF4B (phospho S504)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-eIF4B (phospho S504)抗体[EP2261Y]
参阅全部 eIF4B 一抗 -
描述
兔单克隆抗体[EP2261Y] to eIF4B (phospho S504) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: 293T cell lysate (untreated).
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP2261Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75823于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/25000 - 1/50000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
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说明 |
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WB
1/25000 - 1/50000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
靶标
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功能
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. -
序列相似性
Contains 1 RRM (RNA recognition motif) domain. -
翻译后修饰
Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex. - Information by UniProt
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数据库链接
- Entrez Gene: 1975 Human
- Entrez Gene: 75705 Mouse
- Entrez Gene: 300253 Rat
- Omim: 603928 Human
- SwissProt: P23588 Human
- SwissProt: Q8BGD9 Mouse
- Unigene: 648394 Human
- Unigene: 702041 Human
see all -
别名
- 2310046H11Rik antibody
- AL024095 antibody
- C85189 antibody
see all
图片
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All lanes : Anti-eIF4B (phospho S504) antibody [EP2261Y] (ab75823) at 1/50000 dilution
Lane 1 : 293T cell lysate, untreated
Lane 2 : 293T cell lysate, treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab75823 被引用在 2 文献中.
- Bettegazzi B et al. Casein Kinase 2 dependent phosphorylation of eIF4B regulates BACE1 expression in Alzheimer's disease. Cell Death Dis 12:769 (2021). PubMed: 34349120
- Bettegazzi B et al. eIF4B phosphorylation at Ser504 links synaptic activity with protein translation in physiology and pathology. Sci Rep 7:10563 (2017). PubMed: 28874824