Anti-eIF4B抗体[1F5] (ab129369)
Key features and details
- Mouse monoclonal [1F5] to eIF4B
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG2b
概述
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产品名称
Anti-eIF4B抗体[1F5]
参阅全部 eIF4B 一抗 -
描述
小鼠单克隆抗体[1F5] to eIF4B -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Mouse, Human, Recombinant fragment -
免疫原
Purified recombinant fragment of Human eIF4B expressed in E. Coli.
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阳性对照
- A549, A431, HepG2, HEK293, Jurkat, K562 and MCF-7 cell lysates. HeLa and NIH3T3 cells.
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常规说明
This product was changed from ascites to supernatant. Lot no’s high than GR185156-5 are from Tissue Culture Supernatant
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
1F5 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab129369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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WB | 1/500 - 1/2000. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa). | |
Flow Cyt | 1/200 - 1/400. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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ICC/IF | 1/200 - 1/1000. |
靶标
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功能
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. -
序列相似性
Contains 1 RRM (RNA recognition motif) domain. -
翻译后修饰
Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex. - Information by UniProt
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数据库链接
- Entrez Gene: 1975 Human
- Entrez Gene: 75705 Mouse
- Omim: 603928 Human
- SwissProt: P23588 Human
- SwissProt: Q8BGD9 Mouse
- Unigene: 648394 Human
- Unigene: 702041 Human
- Unigene: 290022 Mouse
see all -
别名
- 2310046H11Rik antibody
- AL024095 antibody
- C85189 antibody
see all
图片
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All lanes : Anti-eIF4B antibody [1F5] (ab129369) at 1/500 dilution
Lane 1 : A549 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HEK293 cell lysate
Lane 5 : HeLa cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : K562 cell lysate
Lane 8 : NIH3T3 cell lysate
Lane 9 : MCF-7 cell lysate
Predicted band size: 69 kDa -
ab129369 at 1/200 dilution (green) or negative control (red) staining eIF4B in HeLa cells by flow cytometry.
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ab129369 at 1/200 dilution staining eIF4B in NIH3T3 cells by immunofluorescence. DNA was stained with DRAQ5 dye (blue) and actin with Alexa 555 phalloidin (red).
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Anti-eIF4B antibody [1F5] (ab129369) at 1/500 dilution + eIF4B recombinant fragment (aa 381-585)
Predicted band size: 69 kDa
The expected molecular weight of the recombinant fragment is 48 kDa.
实验方案
数据表及文件
文献 (0)
ab129369 尚未被引用在任何文献中。