Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (Megakaryocytes)
Specification
Megakaryocytes
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ecl
Bands
Specific: 47 kDa
Exposure
5 minute(s) and 0 second(s)
Additional data
Additional Notes
in the image you can see the result with 10 and 30 ug of total proteins
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 07 2013