Anti-eIF4A2抗体(ab31218)
Key features and details
- Rabbit polyclonal to eIF4A2
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
-
产品名称
Anti-eIF4A2抗体
参阅全部 eIF4A2 一抗 -
描述
兔多克隆抗体to eIF4A2 -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human eIF4A2 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab32473) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab31218于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IP | (1) |
Use at an assay dependent concentration.
|
WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
|
说明 |
---|
IP
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
靶标
-
功能
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. -
序列相似性
Belongs to the DEAD box helicase family. eIF4A subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. - Information by UniProt
-
数据库链接
- Entrez Gene: 1974 Human
- Entrez Gene: 13682 Mouse
- Entrez Gene: 303831 Rat
- Omim: 601102 Human
- SwissProt: Q14240 Human
- SwissProt: P10630 Mouse
- SwissProt: Q5RKI1 Rat
- Unigene: 518475 Human
see all -
别名
- ATP dependent RNA helicase eIF4A 2 antibody
- ATP-dependent RNA helicase eIF4A-2 antibody
- BM-010 antibody
see all
图片
-
Anti-eIF4A2 antibody (ab31218) at 1 µg/ml +
Jurkat whole cell lysate (ab7899) at 20 µg
Secondary
IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands. -
eIF4A2 was immunoprecipitated using 0.5mg Mouse skeletal muscle whole tissue extract, 5µg of Rabbit polyclonal to eIF4A2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse skeletal muscle whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31218.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 47kDa: eIF4A2 . -
All lanes : Anti-eIF4A2 antibody (ab31218) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Testis (Mouse) Tissue Lysate
Lane 3 :Mouse skeletal muscle tissue lysate - total protein (ab29711)
Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lane 5 : Ovary (Mouse) Tissue Lysate
Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 7 : Brain (Rat) Tissue Lysate
Lane 8 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: 51 kDa. We are unsure as to the identity of these extra bands.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (29)
ab31218 被引用在 29 文献中.
- Chen C et al. Translational and post-translational control of human naïve versus primed pluripotency. iScience 25:103645 (2022). PubMed: 35005567
- Faucher-Giguère L et al. High-grade ovarian cancer associated H/ACA snoRNAs promote cancer cell proliferation and survival. NAR Cancer 4:zcab050 (2022). PubMed: 35047824
- Drongitis D et al. Deregulation of microtubule organization and RNA metabolism in Arx models for lissencephaly and developmental epileptic encephalopathy. Hum Mol Genet 31:1884-1908 (2022). PubMed: 35094084
- Huth M et al. NMD is required for timely cell fate transitions by fine-tuning gene expression and regulating translation. Genes Dev 36:348-367 (2022). PubMed: 35241478
- Chen M et al. Dual targeting of DDX3 and eIF4A by the translation inhibitor rocaglamide A. Cell Chem Biol 28:475-486.e8 (2021). PubMed: 33296667