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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger

Anti-Egr1抗体(ab6054)

本 快线抗体 仍未被充份测试和鉴定, 不在质保范围内, 具体请参见 使用条款及细节。
  • Datasheet
Submit a review Submit a question References (2)

Product price, shipping and contact information

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送货和订单信息

Western blot - Anti-Egr1 antibody (ab6054)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Egr1 antibody (ab6054)

Key features and details

  • Rabbit polyclonal to Egr1
  • Suitable for: WB, IHC-P
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Egr1抗体
    参阅全部 Egr1 一抗
  • 描述

    兔多克隆抗体to Egr1

    该产品是 快线抗体,通过亲和纯化得到。对抗原肽有高灵敏度及特异性(ELISA测试)。 Read the terms of use »

  • 宿主

    Rabbit
  • 种属反应性


    预测可用于: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    GEQPYEHLTAESFPDI-C

    conjugated to KLH, corresponding to amino acids 93-108 of Human Egr1.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Expiration date is 1 year from date of opening.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Egr1 protein (ab185397)

应用

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

应用 Ab评论 说明
WB
IHC-P
  • 应用说明
    Tested in peptide ELISA.
    We have detected a band of 58kDa in mouse embryonic fibroblast whole cell lysate, but we have failed to detect a band at 83kDa (expected size from data from other antibodies). We would appreciate any customer feedback for this antibody.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能

      Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
    • 序列相似性

      Belongs to the EGR C2H2-type zinc-finger protein family.
      Contains 3 C2H2-type zinc fingers.
    • 细胞定位

      Nucleus.
    • Target information above from: UniProt accession P18146 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 1958 Human
      • Entrez Gene: 13653 Mouse
      • Entrez Gene: 24330 Rat
      • Omim: 128990 Human
      • SwissProt: P18146 Human
      • SwissProt: P08046 Mouse
      • SwissProt: P08154 Rat
      • Unigene: 326035 Human
      • Unigene: 708393 Human
      • Unigene: 181959 Mouse
      • Unigene: 9096 Rat
      see all
    • 别名

      • AT225 antibody
      • Early growth response 1 antibody
      • Early growth response protein 1 antibody
      • EGR 1 antibody
      • EGR-1 antibody
      • EGR1 antibody
      • EGR1_HUMAN antibody
      • G0S30 antibody
      • KROX 24 antibody
      • KROX24 antibody
      • Nerve growth factor-induced clone A antibody
      • Nerve growth factor-induced protein A antibody
      • NGFI-A antibody
      • NGFIA antibody
      • TIS8 antibody
      • Transcription factor ETR103 antibody
      • Transcription factor Zif268 antibody
      • ZIF 268 antibody
      • ZIF268 antibody
      • Zinc finger protein 225 antibody
      • Zinc finger protein Krox-24 antibody
      • ZNF225 antibody
      see all

    图片

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • Western blot - Anti-Egr1 antibody (ab6054)
      Western blot - Anti-Egr1 antibody (ab6054)
      Anti-Egr1 antibody (ab6054) at 1/1500 dilution + Mouse embryonic fibroblast whole cell lysate at 35 µg

      Secondary
      Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution

      Predicted band size: 57 kDa
      Observed band size: 58 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Egr1 antibody (ab6054)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Egr1 antibody (ab6054)

      ab6054 at 10 µg/ml staining human ovarian cancinoma.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • Datasheet
  • 文献 (2)

    发表研究结果有使用 ab6054?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6054 被引用在 2 文献中.

    • Zhao J  et al. REC8 inhibits EMT by downregulating EGR1 in gastric cancer cells. Oncol Rep 39:1583-1590 (2018). PubMed: 29393474
    • Nadra K  et al. Phosphatidic acid mediates demyelination in Lpin1 mutant mice. Genes Dev 22:1647-61 (2008). WB ; Mouse . PubMed: 18559480

    客户评价及客户问答

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    There are currently no Customer reviews or Questions for ab6054.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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