Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 5%BSA in PBST
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Dilution
1/5000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 185 kDa
Additional data
Additional Notes
The loading amount of lane 1 is 25ug and lane 2 is 50ug.
No other non-specific background bands were seen.
No other non-specific background bands were seen.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 08 2011