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Customer would like to know if these EGFR antibodies are specific for the truncated receptor.
Asked on May 24 2004
Thank you for your patience. I have received the following information thus far: Ab231: In Western blotting ab231 detects a 170kDa band which corresponds to EGFR. The epitope recognized by this antibody is located on the extracellular domain of the EGFR. I belive that the truncated form of EGFR lacks the cytoplasmic and transmembrane domain. If it is indeed this simple, then I would expect the antibody to recognise the truncated form. However, to our knowledge, this antibody has not been tested for reactivity with this form of EGFR and therefore we would be unable to offer any guarantees. Ab2430: Ab2430 is made using a peptide corresponding to AA 1005-1016 of the human EGFR precursor. There are several truncated forms of EGFR and one of the more prevelant is EGFRvIII where amino acids 6-273 are replaced by a glycine residue. This antibody will recognize this variant, but it will also recognize the wild type. The researcher will need to compare the variants sequence to that of the wild type to make sure that this antibody's epitope has not been deleted. Once I receive more information regarding ab5636 and ab30, I will contact you again.
回复于 May 28 2004