Anti-EGFR抗体[ICR10] (ab231)
![Flow Cytometry - Anti-EGFR antibody [ICR10] (ab231)](https://www.abcam.cn/ps/products/0/ab231/Images/ab231-3449-anti-egfr-antibody-icr10-flow-cytometry.jpg "Flow Cytometry - Anti-EGFR antibody [ICR10] (ab231)")
Key features and details
- Rat monoclonal [ICR10] to EGFR
- Suitable for: Inhibition Assay, Flow Cyt, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
概述
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产品名称
Anti-EGFR抗体[ICR10]
参阅全部 EGFR 一抗 -
描述
大鼠单克隆抗体[ICR10] to EGFR -
宿主
Rat -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanInhibition Recombinant fragment
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免疫原
Extracellular domain of Human EGF-receptor from head and neck carcinoma
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表位
ICR10 binds to epitope B, and has an affinity of 6.7x10-9 M. -
阳性对照
- FACS: A431 cells IHC: Breast Carcinoma tissue WB: HN5 cell lysate
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Ion Exchange Chromatography -
纯化说明
Purified IgG prepared by ion exchange chromatography. -
克隆
单克隆 -
克隆编号
ICR10 -
骨髓瘤
unknown -
同种型
IgG2a -
轻链类型
unknown -
研究领域
相关产品
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Affibody® Molecule
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| 应用 | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC/IF |
Human
|
| Inhibition |
Recombinant fragment
|
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Inhibition Assay |
Use at an assay dependent concentration. See ELISA Abreview.
|
|
| Flow Cyt | (1) |
1/50 - 1/100.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
| 说明 |
|---|
|
Inhibition Assay
Use at an assay dependent concentration. See ELISA Abreview. |
|
Flow Cyt
1/50 - 1/100. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
|
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action. -
组织特异性
Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. -
疾病相关
Lung cancer
Inflammatory skin and bowel disease, neonatal, 2 -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197. -
细胞定位
Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF). - Information by UniProt
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数据库链接
- Entrez Gene: 1956 Human
- Omim: 131550 Human
- SwissProt: P00533 Human
- Unigene: 488293 Human
- Unigene: 605083 Human
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别名
- Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
- Cell growth inhibiting protein 40 antibody
- Cell proliferation inducing protein 61 antibody
see all
图片
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Flow Cytometry - Anti-EGFR antibody [ICR10] (ab231)Staining of A431 cells with ab231 visualised with F(ab')2 rabbit anti-rat FITC conjugated secondary.
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![Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [ICR10] (ab231)](https://www.abcam.cn/ps/products/0/ab231/Images/ab231-3448-anti-egfr-antibody-icr10-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [ICR10] (ab231)ICC/IF image of ab231 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab231, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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![Inhibition Assay - Anti-EGFR antibody [ICR10] (ab231)](https://www.abcam.cn/ps/products/0/ab231/Images/ab231-3447-anti-egfr-antibody-icr10-inhibition-assay.jpg)
Inhibition Assay - Anti-EGFR antibody [ICR10] (ab231)ab231 used undiluted in inhibition assay against human recombinant protein EGFR1:Fc to inhibit binding to EGF:Biotin. The primary incubated with sample for 1 hour. The antibody inhibits the binding of biotinylated EGF to coated EGFR:Fc with an IC50 ~ 5 nM.
实验方案
文献 (22)
ab231 被引用在 22 文献中.
- Gerlach JP et al. Combined quantification of intracellular (phospho-)proteins and transcriptomics from fixed single cells. Sci Rep 9:1469 (2019). PubMed: 30728416
- Ortega FG et al. EGFR detection in extracellular vesicles of breast cancer patients through immunosensor based on silica-chitosan nanoplatform. Talanta 194:243-252 (2019). PubMed: 30609526
- Lingemann M et al. The alpha-1 subunit of the Na+,K+-ATPase (ATP1A1) is required for macropinocytic entry of respiratory syncytial virus (RSV) in human respiratory epithelial cells. PLoS Pathog 15:e1007963 (2019). PubMed: 31381610
- Wang H et al. Evaluation of serum extracellular vesicles as noninvasive diagnostic markers of glioma. Theranostics 9:5347-5358 (2019). PubMed: 31410219
- Sinclair JKL et al. Mechanism of Allosteric Coupling into and through the Plasma Membrane by EGFR. Cell Chem Biol 25:857-870.e7 (2018). PubMed: 29731426
