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Signal Transduction Protein Phosphorylation Tyrosine Kinases Receptor Tyrosine Kinases

Anti-EGFR抗体[ICR10] (ab231)

  • Datasheet
  • SDS
Reviews (7)Q&A (5)References (22)

Product price, shipping and contact information

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Flow Cytometry - Anti-EGFR antibody [ICR10] (ab231)
  • Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [ICR10] (ab231)
  • Inhibition Assay - Anti-EGFR antibody [ICR10] (ab231)

Key features and details

  • Rat monoclonal [ICR10] to EGFR
  • Suitable for: Inhibition Assay, Flow Cyt, ICC/IF
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG2a

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概述

  • 产品名称

    Anti-EGFR抗体[ICR10]
    参阅全部 EGFR 一抗
  • 描述

    大鼠单克隆抗体[ICR10] to EGFR
  • 宿主

    Rat
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    Inhibition
    Recombinant fragment
    See all applications and species data
  • 免疫原

    Extracellular domain of Human EGF-receptor from head and neck carcinoma

  • 表位

    ICR10 binds to epitope B, and has an affinity of 6.7x10-9 M.
  • 阳性对照

    • FACS: A431 cells IHC: Breast Carcinoma tissue WB: HN5 cell lysate

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Ion Exchange Chromatography
  • 纯化说明

    Purified IgG prepared by ion exchange chromatography.
  • 克隆

    单克隆
  • 克隆编号

    ICR10
  • 骨髓瘤

    unknown
  • 同种型

    IgG2a
  • 轻链类型

    unknown
  • 研究领域

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • EGF
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Cancer
    • Growth factors
    • EGF
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • Receptor tyrosine kinases
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Cancer
    • Tumor biomarkers
    • Receptors

相关产品

  • Affibody® Molecule

    • Anti-EGFR Affibody® Molecule Agarose Immobilized (ab36039)
  • Alternative Versions

    • FITC Anti-EGFR antibody [ICR10] (ab11400)
    • PE Anti-EGFR antibody [ICR10] (ab27764)
  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
  • Recombinant Protein

    • Recombinant human EGFR protein (Fc Chimera) (ab84002)
  • Related Products

    • Human EGFR ELISA Kit (ERBB) (ab100505)
    • Human soluble EGFR ELISA Kit (ab193764)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
ICC/IF
Human
Inhibition
Recombinant fragment
应用 Ab评论 说明
Inhibition Assay
Use at an assay dependent concentration. See ELISA Abreview.
Flow Cyt (1)
1/50 - 1/100.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF
Use a concentration of 5 µg/ml.
说明
Inhibition Assay
Use at an assay dependent concentration. See ELISA Abreview.
Flow Cyt
1/50 - 1/100.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF
Use a concentration of 5 µg/ml.
  • 应用说明
    Is unsuitable for WB.
  • 靶标

    • 功能

      Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
      Isoform 2 may act as an antagonist of EGF action.
    • 组织特异性

      Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
    • 疾病相关

      Lung cancer
      Inflammatory skin and bowel disease, neonatal, 2
    • 序列相似性

      Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰

      Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
      Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
      Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
    • 细胞定位

      Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
    • Target information above from: UniProt accession P00533 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 1956 Human
      • Omim: 131550 Human
      • SwissProt: P00533 Human
      • Unigene: 488293 Human
      • Unigene: 605083 Human
      • 别名

        • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
        • Cell growth inhibiting protein 40 antibody
        • Cell proliferation inducing protein 61 antibody
        • EGF R antibody
        • EGFR antibody
        • EGFR_HUMAN antibody
        • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
        • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
        • Epidermal growth factor receptor antibody
        • erb-b2 receptor tyrosine kinase 1 antibody
        • ERBB antibody
        • ERBB1 antibody
        • Errp antibody
        • HER1 antibody
        • mENA antibody
        • NISBD2 antibody
        • Oncogen ERBB antibody
        • PIG61 antibody
        • Proto-oncogene c-ErbB-1 antibody
        • Receptor tyrosine protein kinase ErbB 1 antibody
        • Receptor tyrosine-protein kinase ErbB-1 antibody
        • SA7 antibody
        • Species antigen 7 antibody
        • Urogastrone antibody
        • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
        • wa2 antibody
        • Wa5 antibody
        see all

      图片

      • Flow Cytometry - Anti-EGFR antibody [ICR10] (ab231)
        Flow Cytometry - Anti-EGFR antibody [ICR10] (ab231)
        Staining of A431 cells with ab231 visualised with F(ab')2 rabbit anti-rat FITC conjugated secondary.
      • Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [ICR10] (ab231)
        Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [ICR10] (ab231)

        ICC/IF image of ab231 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab231, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

      • Inhibition Assay - Anti-EGFR antibody [ICR10] (ab231)
        Inhibition Assay - Anti-EGFR antibody [ICR10] (ab231)

        ab231 used undiluted in inhibition assay against human recombinant protein EGFR1:Fc to inhibit binding to EGF:Biotin. The primary incubated with sample for 1 hour. The antibody inhibits the binding of biotinylated EGF to coated EGFR:Fc with an IC50 ~ 5 nM.

      实验方案

      • Flow cytometry protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet
      • SDS
    • 文献 (22)

      发表研究结果有使用 ab231?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab231 被引用在 22 文献中.

      • Gerlach JP  et al. Combined quantification of intracellular (phospho-)proteins and transcriptomics from fixed single cells. Sci Rep 9:1469 (2019). PubMed: 30728416
      • Ortega FG  et al. EGFR detection in extracellular vesicles of breast cancer patients through immunosensor based on silica-chitosan nanoplatform. Talanta 194:243-252 (2019). PubMed: 30609526
      • Lingemann M  et al. The alpha-1 subunit of the Na+,K+-ATPase (ATP1A1) is required for macropinocytic entry of respiratory syncytial virus (RSV) in human respiratory epithelial cells. PLoS Pathog 15:e1007963 (2019). PubMed: 31381610
      • Wang H  et al. Evaluation of serum extracellular vesicles as noninvasive diagnostic markers of glioma. Theranostics 9:5347-5358 (2019). PubMed: 31410219
      • Sinclair JKL  et al. Mechanism of Allosteric Coupling into and through the Plasma Membrane by EGFR. Cell Chem Biol 25:857-870.e7 (2018). PubMed: 29731426
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-10 of 12 Abreviews or Q&A

      Immunohistochemistry (Frozen sections) abreview for Anti-EGFR antibody [ICR10]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (CB17/SCID BxPC-3 xenograft)
      Specification
      CB17/SCID BxPC-3 xenograft
      Fixative
      Paraformaldehyde
      Permeabilization
      No
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
      Read More

      Dr. Jennifer Dembinski

      Verified customer

      提交于 May 14 2009

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-EGFR antibody [ICR10]

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue (brain slice)
      Permeabilization
      Yes - Tritonx-100
      Specification
      brain slice
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      提交于 Jan 13 2021

      Immunocytochemistry/ Immunofluorescence abreview for Anti-EGFR antibody [ICR10]

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (184A1 mammary epithelial)
      Permeabilization
      Yes - PBS + 0.25% Triton-X
      Specification
      184A1 mammary epithelial
      Blocking step
      (agent) for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      提交于 Oct 19 2020

      Western blot abreview for Anti-EGFR antibody [ICR10]

      Good
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (A431 cells)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      10 µg
      Specification
      A431 cells
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      提交于 Aug 16 2017

      Flow Cytometry abreview for Anti-EGFR antibody [ICR10]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Human Cell (HCC827 cells)
      Permeabilization
      No
      Gating Strategy
      Live cells (PI negative)
      Specification
      HCC827 cells
      Preparation
      Cell harvesting/tissue preparation method: Trypsinized
      Sample buffer: RPMI1640 with 10% FBS and Penicillin-Streptomycin
      Fixation
      No
      Read More

      Abcam user community

      Verified customer

      提交于 Jul 14 2017

      Question

      I am looking for an EGFR antibody that recognizes the N-term (extracellular domain) of EGFR but does not block EGF binding to EGFR. I need the antibody for flow cytometry experiments.

      Read More

      Abcam community

      Verified customer

      Asked on Sep 26 2014

      Answer

      This antibody recognizes the extracellular domain of EGFR and is validated for flow cytometry. There is data demonstrating the effects of the antibody on binding of EGF to EGFR in the reference at the following link.

      http://onlinelibrary.wiley.com/doi/10.1002/ijc.11055/pdf

      The data in figure 3 shows that ICR10 blocks partially, compared to the medium control. receptor.

      Read More

      Tom Ruyle

      Abcam Scientific Support

      回复于 Sep 26 2014

      Question

      Do you know the isoelectric point of the Rat monoclonal ICR10 antibody, abcam part number ab231?

      Read More

      Abcam community

      Verified customer

      Asked on Oct 08 2012

      Answer

      Most monoclonal antibodies will have an isoelectric point around 6.1, but this is not something that we test except for some of our RabMab antibodies.

      Read More

      Abcam Scientific Support

      回复于 Oct 08 2012

      Immunohistochemistry (Frozen sections) abreview for Anti-EGFR antibody [ICR10]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (tumor cell line xenograft in mouse)
      Specification
      tumor cell line xenograft in mouse
      Fixative
      Acetone
      Permeabilization
      No
      Read More

      Abcam user community

      Verified customer

      提交于 Feb 24 2012

      ELISA abreview for Anti-EGFR antibody [ICR10]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      ELISA
      Sample
      Human Recombinant protein (EGFR1:Fc)
      Specification
      EGFR1:Fc
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
      Type
      Other
      Read More

      Ms. Lakshmi Ganesan

      Verified customer

      提交于 Nov 20 2009

      Question

      Customer would like to know if these EGFR antibodies are specific for the truncated receptor.

      Read More

      Abcam community

      Verified customer

      Asked on May 24 2004

      Answer

      Thank you for your patience. I have received the following information thus far: Ab231: In Western blotting ab231 detects a 170kDa band which corresponds to EGFR. The epitope recognized by this antibody is located on the extracellular domain of the EGFR. I belive that the truncated form of EGFR lacks the cytoplasmic and transmembrane domain. If it is indeed this simple, then I would expect the antibody to recognise the truncated form. However, to our knowledge, this antibody has not been tested for reactivity with this form of EGFR and therefore we would be unable to offer any guarantees. Ab2430: Ab2430 is made using a peptide corresponding to AA 1005-1016 of the human EGFR precursor. There are several truncated forms of EGFR and one of the more prevelant is EGFRvIII where amino acids 6-273 are replaced by a glycine residue. This antibody will recognize this variant, but it will also recognize the wild type. The researcher will need to compare the variants sequence to that of the wild type to make sure that this antibody's epitope has not been deleted. Once I receive more information regarding ab5636 and ab30, I will contact you again.

      Read More

      Abcam Scientific Support

      回复于 May 28 2004

      1-10 of 12 Abreviews or Q&A

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