Application
Western blot
Sample
Mouse Cell lysate - other (macrophage)
Gel Running Conditions
Reduced Denaturing
Loading amount
80 µg
Specification
macrophage
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-T with 0.25% BSA
Dilution
1/500
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~170 kDa
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提交于 Nov 06 2015