重组Anti-EGF抗体[EPR19173] (ab184265)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19173] to EGF
- Suitable for: IHC-Fr, WB, IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
概述
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产品名称
Anti-EGF抗体[EPR19173]
参阅全部 EGF 一抗 -
描述
兔单克隆抗体[EPR19173] to EGF -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse and rat kidney lysates; Mouse urine lysate. IHC-P: Mouse and rat kidney tissues. IHC-Fr: Mouse kidney tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19173 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab184265于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-Fr |
1/500.
Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20) |
|
WB |
1/1000. Detects a band of approximately 160, 97 ,66 ,56 ,45 ,27 kDa (predicted molecular weight: 133 kDa).
|
|
IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
(Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)) |
说明 |
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IHC-Fr
1/500. Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20) |
WB
1/1000. Detects a band of approximately 160, 97 ,66 ,56 ,45 ,27 kDa (predicted molecular weight: 133 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)) |
靶标
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功能
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). -
组织特异性
Expressed in kidney, salivary gland, cerebrum and prostate. -
疾病相关
Hypomagnesemia 4 -
序列相似性
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats. -
翻译后修饰
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 13645 Mouse
- Entrez Gene: 25313 Rat
- SwissProt: P01132 Mouse
- SwissProt: P07522 Rat
- Unigene: 252481 Mouse
- Unigene: 6075 Rat
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别名
- Beta urogastrone antibody
- beta-urogastrone antibody
- EGF antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling EGF with ab184265 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on distal convoluted tubules of mouse kidney [PMID 1930151]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-EGF antibody [EPR19173] (ab184265) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Rat kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 133 kDa
Observed band size: 160,45,56,66,97 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 10 seconds; Lane 2: 30 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID:1636692, 2260661, 7499272 & 2260661).
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Anti-EGF antibody [EPR19173] (ab184265) at 1/5000 dilution + Mouse urine lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 133 kDa
Observed band size: 160,27,45,56,66,97 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID:1636692, 2260661, 7499272 & 2260661).
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Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling EGF with ab184265 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on distal convoluted tubules of rat kidney [PMID 1930151]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling EGF with ab184265 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
No staining on mouse stomach.
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling EGF with ab184265 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Positive staining on mouse kidney was observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (4)
ab184265 被引用在 4 文献中.
- Chu H et al. SERPINE2 feedback regulates EGF/EGFR signaling in human papillary thyroid carcinoma cells. Mol Med Rep 23:N/A (2021). PubMed: 33760135
- Li Y et al. Effects of a Chlorogenic Acid-Containing Herbal Medicine (LASNB) on Colon Cancer. Evid Based Complement Alternat Med 2021:9923467 (2021). PubMed: 34462643
- Du F et al. Forkhead box K2 promotes human colorectal cancer metastasis by upregulating ZEB1 and EGFR. Theranostics 9:3879-3902 (2019). PubMed: 31281520
- Zhang Y et al. Netrin-1 promotes diabetic corneal wound healing through molecular mechanisms mediated via the adenosine 2B receptor. Sci Rep 8:5994 (2018). PubMed: 29662125