Key features and details
- Rabbit polyclonal to EED
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
参阅全部 EED 一抗
特异性ab4469 specifically recognises the four isoforms of EED following the immunoprecipitation of tagged Ezh2 from HEK293 nuclear extract. However, using HEK293 nuclear lysate ab4469 predominantly recognises non-specific bands.
经测试应用适用于: WB, ICC/IFmore details
种属反应性与反应: Human, Recombinant fragment
预测可用于: Mouse, Chicken, Cow, Xenopus laevis
Synthetic peptide corresponding to Human EED aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human Recombinant EED Protein
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度Immunogen affinity purified
功能Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
组织特异性Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer.
序列相似性Belongs to the WD repeat ESC family.
Contains 7 WD repeats.
发展阶段Expression peaks at the G1/S phase boundary.
细胞定位Nucleus. Chromosome. Transiently colocalizes with XIST at inactive X chromosomes.
- Information by UniProt
- eed antibody
- EED protein antibody
- EED, mouse, homolog of antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: EED knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab4469 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab4469 was shown to recognize EED in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when EED knockout samples were examined. Wild-type and EED knockout samples were subjected to SDS-PAGE. ab4469 and ab8245 (loading control to GAPDH) were diluted 1/250 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Rabbit polyclonal to EED (ab4469) on U-2 OS (Human bone osteosarcoma epithelial cell line) cells. Antibody used at 1/100.
U-2 OS cells were fixed with PFA (3.7%), permeabilised with PBS 0.1% Triton. Counterstained with Hoechst. Cells were examined with Axioplan 2 microscope (Zeiss).
Secondary antibody: donkey anti-rabbit (Cy3).
Anti-EED antibody (ab4469) at 1/250 dilution + EED Recombinant Protein (Human) at 0.1 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
ab4469 recognizes the full-length recombinant EED protein which has an expected molecular weight of 80 kDa (tagged).
ab4469 被引用在 24 文献中.
- Lee JW et al. RUNX3 regulates cell cycle-dependent chromatin dynamics by functioning as a pioneer factor of the restriction-point. Nat Commun 10:1897 (2019). PubMed: 31015486
- Pandya P et al. PICOT binding to chromatin-associated EED negatively regulates cyclin D2 expression by increasing H3K27me3 at the CCND2 gene promoter. Cell Death Dis 10:685 (2019). PubMed: 31527584
- Wang J et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. Cell 175:1887-1901.e18 (2018). PubMed: 30550787
- Wan L et al. Phosphorylation of EZH2 by AMPK Suppresses PRC2 Methyltransferase Activity and Oncogenic Function. Mol Cell 69:279-291.e5 (2018). PubMed: 29351847
- Anwar T et al. p38-mediated phosphorylation at T367 induces EZH2 cytoplasmic localization to promote breast cancer metastasis. Nat Commun 9:2801 (2018). PubMed: 30022044