Application
Western blot
Sample
Human Cell lysate - other (Non-small cell lung carcinoma)
Loading amount
20 µg
Specification
Non-small cell lung carcinoma
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk TBST
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-Rabbit HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 180 kDa
Positive control
cytosolic fraction
Negative control
plasma membrane fraction
Additional data
Additional Notes
Specific and at right size. The reason for the PM positive might be due to imperfect experimental procedure rather than the specificity of antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 01 2010